Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments in Physcomitrella patens

Takumi, Sugiyama; Ishida, Tetsuya; Tabei, Nobumitsu; Shigyo, Mikao; Konishi, Mineko; Yoneyama, T.; Yanagisawa, Shuichi

doi:10.1093/jxb/ers042

Cited by 33 publications

(24 citation statements)

References 58 publications

(73 reference statements)

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“…Then, the regions encoding the Dof domains of PpDof3 and PpDof4 in the resultant plasmids were replaced with a 35S promoterdriven hygromycin resistance gene or a 35S promoterdriven kanamycin resistance gene (Sugiyama et al 2012). Transformation of P. patens with these targeting vectors was performed by the polyethylene glycol method, and the transformants were selected on agar plates containing appropriate concentrations of antibiotics according to the method described previously (Sugiyama et al 2012). Genomic Southern blot analysis was performed with EcoRV-digested genomic DNA from the protonema of the wild type and disruptant lines, as described previously (Sugiyama et al 2012).…”

Section: Generation Of P Patens Disruptants and Genomic Southern Blomentioning

confidence: 99%

“…Transformation of P. patens with these targeting vectors was performed by the polyethylene glycol method, and the transformants were selected on agar plates containing appropriate concentrations of antibiotics according to the method described previously (Sugiyama et al 2012). Genomic Southern blot analysis was performed with EcoRV-digested genomic DNA from the protonema of the wild type and disruptant lines, as described previously (Sugiyama et al 2012). PpDof3-and PpDof4-specific DNA probes were prepared by PCR using the gene-specific primers listed in Supplemental Table S1.…”

Section: Generation Of P Patens Disruptants and Genomic Southern Blomentioning

confidence: 99%

“…Quantitative and semi-quantitative PCR were performed as described previously (Sugiyama et al 2012). The primers used are listed in Supplemental Table S1.…”

Section: Quantitative Rt-pcr (Qpcr)mentioning

confidence: 99%

“…Dof transcription factors, which possess plant-specific Dof DNA-binding domains, are present in both vascular and non-vascular plants ( Moreno-Risueno et al 2007;Shigyo et al 2007) and play roles in a variety of biological processes in the plant kingdom (Sugiyama et al 2012;Yanagisawa 2002). Recently, a Dof transcription factor in Arabidopsis thaliana (Arabidopsis) was identified as a transcriptional repressor of CONSTANS (CO), which encodes a master regulator of photoperiodic gene expression and the floral transition from the vegetative phase to the reproductive phase (Imaizumi and Kay 2006;Samach and Coupland 2000;Song et al 2010;Valverde 2011); this factor was designated CYCLING DOF FACTOR 1 (CDF1).…”

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confidence: 99%

“…We previously reported that two Dof proteins from P. patens, PpDof3 and PpDof4, are probably CDF homologues, because they possess amino acid motifs characteristic of CDFs (Sugiyama et al 2012). In spite of the presence of the genes encoding COL and CDF-like proteins in P. patens, a recent comprehensive analysis of the P. patens genome revealed that this moss lacks homologues of FKF1 and GI (Holm et al 2010).…”

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Diurnal expression of CONSTANS-like genes is independent of the function of cycling DOF factor (CDF)-like transcriptional repressors in Physcomitrella patens

Ishida

Takumi

Tabei

et al. 2014

Plant Biotechnology

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The Dof-type transcriptional repressors CYCLING DOF FACTORS (CDFs) directly suppress the expression of CONSTANS (CO), which encodes a key regulator of photoperiodic gene expression, day-length perception and the floral transition in Arabidopsis thaliana. The genes encoding CDF-like (PpDof3 and PpDof4) and CO-like (PpCOL1-PpCOL3) proteins are present in the genome of the moss Physcomitrella patens, although P. patens lacks the genes encoding homologues of FLAVIN-BINDING KELCH REPEAT F-BOX1 (FKF1) and GIGANTEA (GI), which control the stability of CDFs in A. thaliana. In the current study, we investigated whether the functions of PpDof3 and PpDof4 are associated with the expression of PpCOL1-PpCOL3 in P. patens. We found that the diurnal expression patterns of PpDof3 and PpDof4 are similar to those of CDF genes and that like CDF1 from A. thaliana, PpDof3 and PpDof4 function as transcriptional repressors. However, targeted disruptions of PpDof3 and PpDof4 did not affect the expression of PpCOL1-PpCOL3, indicating that the expression of COLs is independent of the functions of PpDof3 and PpDof4 in P. patens.

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Section: Generation Of P Patens Disruptants and Genomic Southern Blomentioning

confidence: 99%

Section: Generation Of P Patens Disruptants and Genomic Southern Blomentioning

confidence: 99%

“…Quantitative and semi-quantitative PCR were performed as described previously (Sugiyama et al 2012). The primers used are listed in Supplemental Table S1.…”

Section: Quantitative Rt-pcr (Qpcr)mentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Diurnal expression of CONSTANS-like genes is independent of the function of cycling DOF factor (CDF)-like transcriptional repressors in Physcomitrella patens

Ishida

Takumi

Tabei

et al. 2014

Plant Biotechnology

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Cycling DOF factor mediated seasonal regulation of sexual reproduction and cold response is not conserved in Physcomitrium patens

Freidinger,

Woodward,

Bùi

et al. 2024

Plant Direct

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Many land plants have evolved such that the transition from vegetative to reproductive development is synchronized with environmental cues. Examples of reproduction in response to seasonal cues can be found in both vascular and nonvascular species; however, most of our understanding of the molecular events controlling this timing has been worked out in angiosperm model systems. While the organism‐level mechanisms of sexual reproduction vary dramatically between vascular and nonvascular plants, phylogenetic and transcriptomic evidence suggest paralogs in nonvascular plants may have conserved function with their vascular counterparts. Given that Physcomitrium patens undergoes sexual reproductive development in response to photoperiodic and cold temperature cues, it is well‐suited for studying evolutionarily conserved mechanisms of seasonal control of reproduction. Thus, we used publicly available microarray data to identify genes differentially expressed in response to temperature cues. We identified two CDF‐like (CDL) genes in the P. patens genome that are the most like the angiosperm Arabidopsis thaliana CDFs based on conservation of protein motifs and diurnal expression patterns. In angiosperms, DNA‐One Finger Transcription Factors (DOFs) play an important role in regulating photoperiodic flowering, regulating physiological changes in response to seasonal temperature changes, and mediating the cold stress response. We created knockout mutations and tested their impact on sexual reproduction and response to cold stress. Unexpectedly, the timing of sexual reproduction in the ppcdl‐double mutants did not differ significantly from wild type, suggesting that the PpCDLs are not necessary for seasonal regulation of this developmental transition. We also found that there was no change in expression of downstream cold‐regulated genes in response to cold stress and no change in freezing tolerance in the knockout mutant plants. Finally, we observed no interaction between PpCDLs and the partial homologs of FKF1, an A. thaliana repressor of CDFs. This is different from what is observed in angiosperms, which suggests that the functions of CDF proteins in angiosperms are not conserved in P. patens.

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Insights into structural and functional diversity of Dof (DNA binding with one finger) transcription factor

Gupta

Malviya

Kushwaha

et al. 2015

Planta

147

110

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The structural, functional and in-silico studies of Dof transcription factor attempted so far reveals immense opportunity to analyze the plant genomes in terms of number of Dof genes and discuss in light of the evolution. The multiple functions of Dof genes needs to explored for crop improvement. Transcription factors play a very vital role in gene regulation at transcriptional level and are being extensively studied across phylas. In recent years, sequencing of plant genomes has led to genome-wide identification and characterizations of diverse types of plant-specific transcription factor gene family providing key insights into their structural and functional diversity. The DNA binding with one finger (Dof), a class belonging to C2H2-type zinc finger family proteins, is a plant-specific transcription factor having multiple roles such as seed maturation and germination, phytohormone and light-mediated regulation and plant responses to biotic and abiotic stresses. Dof proteins are present across plant lineage, from green algae to higher angiosperm, and represent a unique class of transcription factor having bifunctional binding activities, with both DNA and proteins, to regulate the complex transcriptional machinery in plant cells. The structural and functional diversity of the Dof transcription factor family along with the bioinformatics analysis highlighting the phylogeny of Dof families is reviewed in light of its importance in plant biotechnology for crop improvement.

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Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments in Physcomitrella patens

Cited by 33 publications

References 58 publications

Diurnal expression of <i>CONSTANS-like</i> genes is independent of the function of cycling DOF factor (CDF)-like transcriptional repressors in <i>Physcomitrella patens</i>

Diurnal expression of <i>CONSTANS-like</i> genes is independent of the function of cycling DOF factor (CDF)-like transcriptional repressors in <i>Physcomitrella patens</i>

Cycling DOF factor mediated seasonal regulation of sexual reproduction and cold response is not conserved in Physcomitrium patens

Insights into structural and functional diversity of Dof (DNA binding with one finger) transcription factor

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