Involvement of Quinolinate Phosphoribosyl Transferase in Promotion of Potato Growth by a
            <i>Burkholderia</i>
            Strain

Wang, Keri; Conn, K. L.; Lazarovits, George

doi:10.1128/aem.72.1.760-768.2006

Cited by 27 publications

(24 citation statements)

References 50 publications

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“…Burkholderia phytofirmans PsJN T is a prominent and efficient plant growth-promoting endophyte (2,5,9), which was isolated from Glomus vesiculiferum-infected onion roots (4,7).…”

Section: Burkholderia Phytofirmans Psjnmentioning

confidence: 99%

Complete Genome Sequence of the Plant Growth-Promoting Endophyte Burkholderia phytofirmans Strain PsJN

et al. 2011

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Burkholderia phytofirmans PsJNT is able to efficiently colonize the rhizosphere, root, and above-ground plant tissues of a wide variety of genetically unrelated plants, such as potatoes, canola, maize, and grapevines. Strain PsJN shows strong plant growth-promoting effects and was reported to enhance plant vigor and resistance to biotic and abiotic stresses. Here, we report the genome sequence of this strain, which indicates the presence of multiple traits relevant for endophytic colonization and plant growth promotion.

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“…Burkholderia phytofirmans PsJN T is a prominent and efficient plant growth-promoting endophyte (2,5,9), which was isolated from Glomus vesiculiferum-infected onion roots (4,7).…”

Section: Burkholderia Phytofirmans Psjnmentioning

confidence: 99%

Complete Genome Sequence of the Plant Growth-Promoting Endophyte Burkholderia phytofirmans Strain PsJN

et al. 2011

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“…Nicotinamidase activity was determined as described by Wang and Pichersky (2007). QPRT was assayed as described by Wang et al (2006). NAD synthase was assayed as described by Wagner and Wagner (1985), with the exception that the NAD produced was determined enzymatically as described by Gibon and Larher (1997).…”

Section: Nad Biosynthesis Enzyme Activity Assaymentioning

confidence: 99%

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

et al. 2008

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Leaf senescence in Arabidopsis thaliana is a strict, genetically controlled nutrient recovery program, which typically progresses in an age-dependent manner. Leaves of the Arabidopsis onset of leaf death5 (old5) mutant exhibit early developmental senescence. Here, we show that OLD5 encodes quinolinate synthase (QS), a key enzyme in the de novo synthesis of NAD. The Arabidopsis QS was previously shown to carry a Cys desulfurase domain that stimulates reconstitution of the oxygen-sensitive Fe-S cluster that is required for QS activity. The old5 lesion in this enzyme does not affect QS activity but it decreases its Cys desulfurase activity and thereby the long-term catalytic competence of the enzyme. The old5 mutation causes increased NAD steady state levels that coincide with increased activity of enzymes in the NAD salvage pathway. NAD plays a key role in cellular redox reactions, including those of the tricarboxylic acid cycle. Broad-range metabolite profiling of the old5 mutant revealed that it contains higher levels of tricarboxylic acid cycle intermediates and nitrogen-containing amino acids. The mutant displays a higher respiration rate concomitant with increased expression of oxidative stress markers. We postulate that the alteration in the oxidative state is integrated into the plant developmental program, causing early ageing of the mutant.

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“…These growth conditions were used for all experiments unless specified otherwise. The leaf buds generated were dipped into PsJN bacterial suspension for 1 min (Wang et al 2006). They were then dried in a sterile petri dish and transferred into test tubes containing growth media at pH 6.…”

Section: Generation and Maintenance Of Plant Materials And Plant Inocmentioning

confidence: 99%

“…The bacteria were streaked onto nutrient broth agar medium and incubated at 30°C for 48 h. A single colony was then transferred into a test tube (height 15 cm × width 1.5 cm, VWR) containing 5 mL of nutrient broth culture and was incubated overnight at 30°C on a shaker. The PsJN bacterial culture was used to inoculate newly grown leaf buds under sterile conditions (Wang et al 2006).…”

Section: Bacterium Strain and Growth Conditionsmentioning

confidence: 99%

“…Our laboratory previously showed that a PsJN mutant (H41) in the nadC gene is capable of colonizing potato plants but does not promote growth. This gene encodes for a quinolinate phosphoribosyltransferase, a key enzyme in de novo NAD + synthesis (Wang et al 2006). However, owing to the involvement of NAD + in multiple biochemical processes at many key regulatory steps, known as pleiotropic effects, it is not known whether it has a direct role in PsJN growth promotion.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of hexokinase1 in plant growth promotion as mediated byBurkholderia phytofirmans

Park

Lazarovits²

2014

Can. J. Microbiol.

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Potato plantlets inoculated with strain PsJN of the bacterium Burkholderia phytofirmans exhibit consistent and significant increases in plant growth under in vitro conditions, when compared with uninoculated plants. The greatest influence on the degree and type of growth enhancement that develops has been shown to be mediated by the sugar concentration in the agar media. Bacterial growth promotion has been suggested in other studies to be regulated by the sugar sensor enzyme hexokinase1, the role of which is activation of glucose phosphorylation. In this present study, we examined the co-relationship between root and stem development in potato plants treated with PsJN and the activity of hexokinase1. Plants grown in the presence of 1.5% and 3% sucrose showed increased levels of hexokinase1 activity only in the roots of inoculated plants, suggesting that the increased enzyme levels may be associated with root growth. Analysis for mRNA using reverse transcriptase did not reveal any significant differences in transcription levels of the gene between inoculated and uninoculated plants. When PsJN-inoculated plants were grown in 1.5% and 3% concentrations of glucose and fructose, stem height and mass, leaf number, root mass, and overall biomass increased. No growth promotion occurred when PsJN-inoculated plants were grown in 3% maltose. Subsequently, a hexokinase1 activity assay showed that PsJN-induced growth of potato plants was found to only occur when plants were grown in the presence of sugars that are recognized by the plant hexokinase1. The results suggest that PsJN may enhance sugar uptake in plants by direct or indirect stimulation of hexokinase1 activity in roots and this results in enhanced overall plant growth.

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Involvement of Quinolinate Phosphoribosyl Transferase in Promotion of Potato Growth by a Burkholderia Strain

Cited by 27 publications

References 50 publications

Complete Genome Sequence of the Plant Growth-Promoting Endophyte Burkholderia phytofirmans Strain PsJN

Complete Genome Sequence of the Plant Growth-Promoting Endophyte Burkholderia phytofirmans Strain PsJN

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

Involvement of hexokinase1 in plant growth promotion as mediated byBurkholderia phytofirmans

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