Involvement of rho p21 in the GTP-enhanced calcium ion sensitivity of smooth muscle contraction.

Hirata, Ken‐ichi; Kikuchi, Akira; Sasaki, Takuya; Kuroda, Shinya; Kaibuchi, Kozo; Matsuura, Yoshiharu; Seki, Hanae; Saida, Kooichi; Takai, Yoshimi

doi:10.1016/s0021-9258(19)50337-4

Cited by 427 publications

(41 citation statements)

References 35 publications

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“…Thus, the most reasonable explanation for our observations is that caffeine treatment leads to reduced intracellular [Ca 2T ], which in turn potentiates the effect of injected antibodies. Interaction of the Rho family of GTPases with Ca 2+ signaling has also been reported in animal and fungal cells (Miyamoto et al, 1987;Bender and Pringle, 1989;Hirata et al, 1992;Gronroos et al, 1996;Peppelenbosch et al, 1996).…”

Section: Min Aftermentioning

confidence: 83%

Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth

Lin¹,

Yang²

1997

The Plant Cell

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Microinjection of anti-Rop1Ps antibodies was used to assess the function of a tip-localized Rho-type GTPase, Rop, in controlling pollen tube growth. Injected antibodies induced sustained growth arrest within 1 to 2 min after injection but did not affect cytoplasmic streaming. Coinjection with Rop rescued antibody-induced growth inhibition, indicating that injected antibodies specifically block the activity of Rop GTPases. Antibody-induced inhibition was significantly enhanced in the presence of a lower threshold of extracellular [Ca2+] or a subinhibitory dosage of caffeine. In contrast, injection of the C3 toxin, which inactivates a different Rho-type GTPase, arrested tube elongation 10 to 20 min after injection. C3-induced growth arrest was accompanied by the cessation of cytoplasmic streaming. These data suggest that Rho-type GTPases play a pivotal role in the control of pollen tube elongation. We propose that Rop may regulate a Ca2+-dependent pathway involved in vesicle docking/fusion, whereas a C3-sensitive Rho GTPase may mediate cytoplasmic streaming.

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Section: Min Aftermentioning

confidence: 83%

Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth

Lin¹,

Yang²

1997

The Plant Cell

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“…The RhoA protein is a well-known member of the p21 Ras superfamily of small GTPases, which shuttles between an inactive GDP-bound state and an active GTP-bound state and exhibits intrinsic GTPase activities. RhoA regulates signal transduction from cell surface receptors to intracellular target molecules and is involved in a variety of biological processes, including cell morphology (Paterson et al 1990), motility (Takaishi et al 1993), smooth muscle contraction (Hirata et al 1992), and tumor progression (Prendergast et al 1995), and RhoA also acts as a molecular switch. Previous reports have shown that overexpression of RhoA facilitated the accumulation of RhoA protein in the cell membrane and led to further RhoA activation (Horiuchi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer

Wang

Sun

Yang

et al. 2013

J Histochem Cytochem.

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The human DEK proto-oncogene is a nuclear protein with suspected roles in human carcinogenesis. DEK appears to function in several nuclear processes, including transcriptional regulation and modulation of chromatin structure. To investigate the clinicopathological significance of DEK in patients with non-small cell lung cancer (NSCLC), we analyzed DEK immunohistochemistry in 112 NSCLC cases. The results showed that DEK was overexpressed mainly in the nuclear compartment of tumor cells. In squamous cell carcinoma, DEK-positive expression occurred in 47.9% (23/48) of cases, and in lung adenocarcinoma, DEK-positive expression occurred in 67.2% (43/64) of cases and correlated with differentiation, p-TNM stage, and nodal status. Moreover, in lung adenocarcinoma, DEK expression was significantly higher compared with DEK expression in squamous cell carcinoma. Kaplan-Meier analysis showed that patients with low DEK expression had higher overall survival compared with patients with high DEK expression. Depleting DEK expression inhibited cellular proliferation and migration. Furthermore, in DEK-depleted NSCLC cells, we found that RhoA expression was markedly reduced; in conjunction, active RhoA-GTP levels and the downstream effector phosphorylated MLC2 were also reduced. Taken together, DEK depletion inhibited cellular migration in lung cancer cell lines possibly through inactivation of the RhoA/ROCK/MLC signal transduction pathway.

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“…For example, eight years ago it was found that injection into fibroblasts of antibodies raised against MLC kinase induced a decrease in MLC phosphorylation accompanied by the loss of stress fibres [27]. Lysophosphatidic acid (LPA) rapidly stimulates MLC phosphorylation, and various inhibitors of MLC kinase have been reported to prevent LPA-and Rhoinduced contractility and stress-fibre formation [26,28]. Furthermore, it has been shown that Rho is involved in regulating smooth-muscle cell contraction, a process dependent on MLC phosphorylation [29].…”

Section: Rho and The Actin Cytoskeletonmentioning

confidence: 99%

Rho: theme and variations

1996

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In addition to their roles in organizing the actin cytoskeleton, members of the Rho family of GTP-binding proteins have recently been implicated in a plethora of other functions, including the activation of kinase cascades and transcription factors, and the control of endocytosis and secretion. Alongside this expansion is proposed functions has been the identification of multiple target proteins that interact directly with Rho, Rac or Cdc42. Molecular connections are now being made along the signalling pathways activated by members of the Rho family.

show abstract

Involvement of rho p21 in the GTP-enhanced calcium ion sensitivity of smooth muscle contraction.

Cited by 427 publications

References 35 publications

Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth

Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth

DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer

Rho: theme and variations

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