Involvement of Sox9a in chondrogenesis and gonadal development in teleost Nile tilapia (&lt;i&gt;Oreochromis niloticus&lt;/i&gt;)

Li, Xiaoyan; Tang, Yaohao; Deng, Wanyue; Zheng, Yue; Wang, L.; He, Xue; Xie, Qingping; Li, Yueqin; Deng, Li; Wang, Deshou; Wei, Ling

doi:10.24272/j.issn.2095-8137.2023.024

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…The expression and functional data combined thus suggest a different partitioning of functions among soxE family genes (specifically sox9 and sox10 ) in cichlids compared to other teleosts. We posit that sox10a acquired an essential role in chondrogenesis in the cichlid lineage, a function performed by sox9 genes in other teleosts (Cresko et al, 2003; Nagao et al, 2018; Yan et al, 2005), possibly overlapping with the role of sox9a in cichlids (Li et al, 2023) (Fig. 7) .…”

Section: Discussionmentioning

confidence: 91%

“…This suggests a different partitioning of functions among soxE family genes (specifically sox9 and sox10 ) in cichlids compared to other teleosts. We posit that sox10-like acquired an essential role in chondrogenesis in the cichlid lineage, a function ancestrally performed by sox9 in other teleosts 46,55,57 , possibly overlapping with the role of sox9a in cichlids 54 . In contrast, cichlid sox10 appears to have retained its function in pigmentation development, akin to its orthologues in other teleost lineages.…”

Section: Discussionmentioning

confidence: 93%

“…In these teleosts, pigmentation was severely reduced but cartilage development remained unaffected (similarly to other vertebrates 52,53 ). In fact, the chondrogenesis aberration seen in sox10-like mutants is more reminiscent of the effects of sox9a homozygous mutation in Nile tilapia 54 and zebrafish 55 , as well as sox9 in mice 56 . This suggests a different partitioning of functions among soxE family genes (specifically sox9 and sox10 ) in cichlids compared to other teleosts.…”

Section: Discussionmentioning

confidence: 99%

“…In these teleosts, pigmentation was severely reduced, but cartilage development remained unaffected, similar to other vertebrates (Honoré et al, 2003; Kapur, 1999). The chondrogenesis aberration seen in sox10a mutants is more reminiscent of the effects of sox9a homozygous mutation in Nile tilapia (Li et al, 2023) and zebrafish (Yan et al, 2005), as well as sox9 in mice (Wagner et al, 1994) (Fig. 7, Supplementary Table 5) .…”

Section: Discussionmentioning

confidence: 99%

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Genetic and developmental divergence in the neural crest programme between cichlid fish species

Marconi,

Vernaz,

Karunaratna

et al. 2024

Preprint

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Neural crest (NC) is a vertebrate-specific embryonic progenitor cell population at the basis of important vertebrate features such as the craniofacial skeleton and pigmentation patterns. Despite the wide-ranging variation of NC-derived traits across vertebrates, the contribution of NC to species diversification remains largely unexplored. Here, by leveraging the adaptive diversity of African Great Lakes' cichlid species, we combined comparative transcriptomics and population genomics to investigate the role of NC development in morphological diversification. Our analysis revealed substantial differences in transcriptional landscapes across somitogenesis, an embryonic period coinciding with NC development and migration. Notably, several NC-related gene expression clusters showed both species-specific divergence in transcriptional landscapes and signatures of positive selection. Specifically, we identified two paralogs of the sox10 gene as prime NC-related candidates contributing to interspecific morphological variation, which displayed remarkable spatio-temporal expression variation in cichlids. Finally, through CRISPR-KO mutants, we experimentally validated the functional divergence between sox10 paralogs, with the acquisition of a novel role in cichlid skeletogenesis by sox10-like. Our study demonstrates the central role of NC-related processes - in particular those controlled by sox10s - in generating morphological diversification among closely-related species and lays the groundwork for further investigations into the mechanisms underpinning vertebrate NC diversification.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic and developmental divergence in the neural crest programme between cichlid fish species

Marconi,

Vernaz,

Karunaratna

et al. 2024

Preprint

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Abnormal eyes and spine development in zebrafish (Danio rerio) embryos and larvae induced by triphenyltin

Zhang,

Luo,

Gao

et al. 2024

Science of The Total Environment

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Genetic and Developmental Divergence in the Neural Crest Program between Cichlid Fish Species

Marconi,

Vernaz,

Karunaratna

et al. 2024

Molecular Biology and Evolution

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Neural crest (NC) is a vertebrate-specific embryonic progenitor cell population at the basis of important vertebrate features such as the craniofacial skeleton and pigmentation patterns. Despite the wide-ranging variation of NC-derived traits across vertebrates, the contribution of NC to species diversification remains underexplored. Here, leveraging the adaptive diversity of African Great Lakes’ cichlid species, we combined comparative transcriptomics and population genomics to investigate the evolution of the NC genetic program in the context of their morphological divergence. Our analysis revealed substantial differences in transcriptional landscapes across somitogenesis, an embryonic period coinciding with NC development and migration. This included dozens of genes with described functions in the vertebrate NC gene regulatory network, several of which showed signatures of positive selection. Among candidates showing between-species expression divergence, we focused on teleost-specific paralogs of the NC-specifier sox10 (sox10a and sox10b) as prime candidates to influence NC development. These genes, expressed in NC cells, displayed remarkable spatio-temporal variation in cichlids, suggesting their contribution to inter-specific morphological differences, such as craniofacial structures and pigmentation. Finally, through CRISPR/Cas9 mutagenesis, we demonstrated the functional divergence between cichlid sox10 paralogs, with the acquisition of a novel skeletogenic function by sox10a. When compared to teleost models zebrafish and medaka, our findings reveal that sox10 duplication, although retained in most teleost lineages, had variable functional fates across their phylogeny. Altogether, our study suggests that NC-related processes – particularly those controlled by sox10s – are involved in generating morphological diversification between species and lays the groundwork for further investigations into the mechanisms underpinning vertebrate NC diversification.

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Involvement of Sox9a in chondrogenesis and gonadal development in teleost Nile tilapia (<i>Oreochromis niloticus</i>)

Cited by 3 publications

References 9 publications

Genetic and developmental divergence in the neural crest programme between cichlid fish species

Genetic and developmental divergence in the neural crest programme between cichlid fish species

Abnormal eyes and spine development in zebrafish (Danio rerio) embryos and larvae induced by triphenyltin

Genetic and Developmental Divergence in the Neural Crest Program between Cichlid Fish Species

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