Involvement of temporomandibular joint P2X3 and P2X2/3 receptors in carrageenan-induced inflammatory hyperalgesia in rats

Teixeira, Juliana Maia; Oliveira, Maria Cláudia G.; Nociti, Francisco Humberto; Clemente-Napimoga, Juliana Trindade; Pelegrini-da-Silva, Adriana; Parada, Carlos Amílcar; Tambeli, Cláudia Herrera

doi:10.1016/j.ejphar.2010.06.008

Cited by 47 publications

(36 citation statements)

References 60 publications

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“…Further support comes from other observations: 1) Endogenous ATP, acting at P2X3 and P2X2/3 receptors, contributes to the development of inflammatory hyperalgesia in the TMJ (Teixeira et al 2010) and cutaneous tissues (Oliveira et al 2009), as well as tooth pulp (Chiang et al 2005; Hu et al 2002); 2) Allodynia evoked in rats after spinal nerve ligation can also be attenuated by treatment with TNP-ATP (Tsuda et al 2003); 3) Intraperitoneal administration of TNP-ATP blocks visceral nociception in the abdominal constriction assay (Honore et al 2002); and 4) Intrathecal administration of TNP-ATP attenuates hyperalgesia in mice induced by the P2X3 agonist αβ-meATP, as well as the pronociceptive effect of hind paw formalin and capsaicin injections (Tsuda et al 1999). In addition, ionotropic purinergic P2X3 receptors are expressed in nerve fibers of the dental pulp (Jiang and Gu, 2002), consistent with a role for ATP signaling in dental nociception.…”

Section: Discussionmentioning

confidence: 79%

Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study

Kumar

Cherkas

Chiang

et al. 2012

Neurochemistry International

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Our electrophysiological studies have shown that both purinergic and glutamatergic receptors are involved in central sensitization of nociceptive neurons in the medullary dorsal horn (MDH). Here we assessed the effects of intrathecal administration of apyrase (a nucleotide degrading enzyme of endogenous adenosine 5-triphosphate [ATP]), a combination of apyrase and 1,3-dipropyl-8-cyclopentylxanthine (DPCPX, an adenosine A1 receptor antagonist), or 2,3-O-2,4,6-trinitrophenyl-adenosine triphosphate (TNP-ATP, a P2X1, P2X3, P2X2/3 receptor antagonist) on the release of glutamate in the rat MDH evoked by application of mustard oil (MO) to the molar tooth pulp. In vivo microdialysis was used to dialyse the MDH every 5 min, and included 3 basal samples, 6 samples after drug treatment and 12 samples following application of MO. Tooth pulp application of MO induced a significant increase in glutamate release in the MDH. Superfusion of apyrase or TNP-ATP alone significantly reduced the MO-induced glutamate release in the MDH, as compared to vehicle. Furthermore, the suppressive effects of apyrase on glutamate release were reduced by combining it with DPCPX. This study demonstrates that application of an inflammatory irritant to the tooth pulp induces glutamate release in the rat MDH in vivo that may be reduced by processes involving endogenous ATP and adenosine.

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Section: Discussionmentioning

confidence: 79%

Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study

Kumar

Cherkas

Chiang

et al. 2012

Neurochemistry International

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“…P2X3 forms as a homomer or can form as a heteromer with P2X2 [8], and both forms are involved in articular pain and hyperalgesia [9–11]. Specifically, P2X3 and P2X2/3 receptors are localized on peripheral and central terminals of unmyelinated C-fiber and thinly myelinated Aδ sensory afferents [12–14].…”

Section: Introductionmentioning

confidence: 99%

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

et al. 2016

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Osteoarthritis (OA) is a degenerative and progressive disease, characterized by cartilage breakdown and by synovial membrane inflammation, which results in disability, joint swelling and pain. The purinergic P2X3 and P2X2/3 receptors contribute to development of inflammatory hyperalgesia, participate in arthritis processes in the knee joint and are expressed in chondrocytes and nociceptive afferent fibers innervating the knee joint. In this study, we hypothesized that P2X3 and P2X2/3 receptors activation by endogenous ATP (adenosine 5′-triphosphate) induces articular hyperalgesia in the knee joint of male and female rats through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration. We found that the blockade of articular P2X3 and P2X2/3 receptors significantly attenuated carrageenan-induced hyperalgesia in the knee joint of male and estrus female rats in a similar manner. The carrageenan-induced knee joint inflammation increased the expression of P2X3 receptors in chondrocytes of articular cartilage. Further, the blockade of articular P2X3 and P2X2/3 receptors significantly reduced the increased concentration of TNF-α, IL-6 and CINC-1 and the neutrophil migration induced by carrageenan. These findings indicate that P2X3 and P2X2/3 receptors activation by endogenous ATP is essential to hyperalgesia development in the knee joint through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration.

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“…Local injection of CFA (Shinoda et al, 2005;Tashiro et al, 2009), capsaicin (Honda et al, 2008), carrageenan (Teixeira et al, 2010), or formalin (Wu et al, 2009) induces local inflammation in the trigeminal region and results in pain-related behavior, including mechanical and heat hypersensitivity at the inflamed site. However, these models assessed changes in mechanical or heat sensitivity at the inflamed site; few models inducing ectopic inflammatory pain in the noninflamed adjacent division of the trigeminal nerve have been developed in the orofacial region.…”

Section: Model Of Ectopic Trigeminal Inflammatory Painmentioning

confidence: 99%

Nerve Growth Factor Contribution via Transient Receptor Potential Vanilloid 1 to Ectopic Orofacial Pain

Shinoda¹,

Asano²,

Omagari³

et al. 2011

J. Neurosci.

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It is well known that oral inflammation causes tenderness in temporomandibular joints or masseter muscles. The exact mechanism of such an orofacial ectopic hyperalgesia remains unclear. Here, we investigated the functional significance of interaction of nerve growth factor (NGF) and transient receptor potential vanilloid 1 (TRPV1) in relation to heat hyperalgesia in the whisker pad skin caused by complete Freund's adjuvant (CFA) injection into the lower lip. CFA injection induced heat hyperalgesia of the ipsilateral whisker pad skin. Moreover, it leads to enhancement of spontaneous activity and heat responses in trigeminal ganglion (TG) neurons that was elicited by heat stimulation of the whisker pad skin. The heat hyperalgesia was dose-dependently reversed by intraperitoneal TRPV1 antagonist administration, also diminished by neutralizing anti-NGF antibody administration into the lower lip and intraganglionic administration of K252a, a tyrosine kinase receptor inhibitor. Nerve fibers in bundle of mandibular nerve and TG neurons that innervates the whisker pad skin and lower lip both expressed labeled NGF, which was administrated into the lower lip. Moreover, the NGF concentrations in ophthalmic-maxillary and mandibular divisions of the TG increased after CFA injection into the lower lip. The number of TRPV1-positive neurons that innervates the whisker pad skin and lower lip was increased after CFA injection into the lower lip, and this increase was annulled by anti-NGF administration. The present findings suggest that inflammation in the lower lip induces release of NGF that regulates TRPV1 expression in TG neurons. This TRPV1 overexpression may underlie ectopic heat hyperalgesia in the whisker pad skin.

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Involvement of temporomandibular joint P2X3 and P2X2/3 receptors in carrageenan-induced inflammatory hyperalgesia in rats

Cited by 47 publications

References 60 publications

Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study

Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

Nerve Growth Factor Contribution via Transient Receptor Potential Vanilloid 1 to Ectopic Orofacial Pain

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