Involvement of the Antioxidative System in Tomato Response to Fusaric Acid Treatment

Kużniak, Elźbieta; Patykowski, Jacek; Urbanek, H.

doi:10.1046/j.1439-0434.1999.00392.x

Cited by 14 publications

(17 citation statements)

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“…The enhanced production of reactive oxygen species is often recognized as the early response activated in plant‐pathogen interactions (Baker and Orlandi, 1995). Similar to the results obtained for FA‐treated tomato leaves (Kuźniak et al., 1999), there was an increase in the extracellular O 2 − production in suspension cultures. It occurred at about the same time as the maximum alkalinization response, so the NADPH‐oxidase catalysed O 2 − generation could probably participate in the alkalinization as discussed by Wojtaszek (1997).…”

Section: Discussionsupporting

confidence: 85%

“…The previous results obtained with tomato leaves showed a lasting activation of H 2 O 2 production concomitant with CAT and APX activity decrease (Kuźniak et al., 1999). By contrast, in cell cultures, FA treatment did not influence significantly either H 2 O 2 or APX.…”

Section: Discussionmentioning

confidence: 61%

“…Therefore the possibility that reactive oxygen species might also be involved in the response to FA has been of interest. In a previous work, a lasting activation of O 2 – and H 2 O 2 production as well as modifications in the activities of enzymes related to H 2 O 2 metabolism in FA‐treated tomato leaves were found (Kuźniak et al., 1999). In order to further evaluate the involvement of both the oxidative stress and the antioxidant system in response of tomato to FA as well as the levels of reactive oxygen species, O 2 – and H 2 O 2 , the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (POD), dehydroascorbate reductase (DHAR) and ascorbate content in tomato cell cultures have been examined.…”

Section: Introductionmentioning

confidence: 80%

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Effects of Fusaric Acid on Reactive Oxygen Species and Antioxidants in Tomato Cell Cultures

Kużniak¹

2001

Journal of Phytopathology

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Generation of O2– and H2O2 as well as the activities of superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, dehydroascorbate reductase and ascorbate content were studied in tomato cell cultures in response to fusaric acid – a nonspecific toxin of phytopathogenic Fusarium species. Toxin treatment resulted in decreased cell viability which was preceded by culture medium alkalinization up to 0.65 pH unit and enhanced extracellular O2– production. The H2O2 level was not significantly affected. In toxin‐treated cultures, a transient, significant increase occurred in intracellular superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase activities. Fusaric acid‐induced ascorbate turnover modulation led to up to a twofold increase in dehydroascorbic acid accumulation, and a decrease in the associated ascorbate redox ratio. It was concomitant with a significant decrease in dehydroascorbate reductase activity. These results support previous observations that the pro‐ and anti‐oxidant systems are involved in response to fusaric acid treatment although differential response of H2O2 and its metabolism‐related enzymes between the whole leaf and cell culture assays was found.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 80%

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Effects of Fusaric Acid on Reactive Oxygen Species and Antioxidants in Tomato Cell Cultures

Kużniak¹

2001

Journal of Phytopathology

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“…For H 2 O 2 , the quantification was done as described by Kuzniak et al (1999) with slight modifications. Frozen powder (approximately 50 mg) was homogenised in 1 ml of a reagent mixture (50 m M potassium phosphate pH 7.0, 0.05% guaiacol and 10 units ml −1 horseradish POX).…”

Section: Methodsmentioning

confidence: 99%

Catalase inhibition alters suberization and wound healing in potato (Solanum tuberosum) tubers

Bajji

Mahmoud

Gastiny

et al. 2007

Physiologia Plantarum

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In response to wounding, potato (Solanum tuberosum L.) tubers generate hydrogen peroxide (H 2 O 2 ) in association with suberization, a critical phase of the wound-healing process. In the present study, the effect of aminotriazole (AT), a catalase (CAT, EC 1.11.1.6) inhibitor, on cut tubers was investigated using fresh weight (FW) loss and pathogen attack symptoms as indicators of wound-healing efficiency. Seven days after treatment, AT-treated tuber halves lost more FW and developed infection signs compared with the controls. Thiourea, another CAT inhibitor, as well as exogenous H 2 O 2 treatments induced the same effects as AT suggesting that the alteration of the wound healing may be caused by CAT inhibition and the resulting accumulation of H 2 O 2 . Using transgenic tubers, FW losses 1 week after wounding were either higher (CAT repression) or lower (CAT overexpression) than those of the wildtype. When tuber halves were allowed to wound heal for different periods before treatment, AT had no effect on the progress of their wound healing if wound-healed for at least 3 days. This implies that AT may affect early woundhealing-related events, especially those occurring before or during suberization. A time-course analysis of the effects of AT treatment on wounded tuber tissues revealed that AT prevented the deposition of the polyphenolic domain of suberin in association with CAT inhibition and H 2 O 2 accumulation. These data are important in identifying factors that may be required to regulate suberization and contribute to a better understanding of this critical process to hasten its rate and limit wound-related losses in stored potato tubers.

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“…For example, fungi of the genus Fusarium produce a variety of biologically active metabolites in culture ®ltrate (CF); this CF is reported to be toxic to cotyledons, germinating seeds and plants of many vegetable species (Jin et al 1996, Matsumoto et al 1999a). Fusaric acid (5-n-butylpicolinic acid; FA), produced by several fungi of the genus Fusarium associated with wilt of banana, cotton, pea, tomato, and other plants, aects membrane permeability, increasing leakage of potassium and other electrolytes, and inhibits respiration (McLean 1996, Kuzniak et al 1999.…”

mentioning

confidence: 99%

A first attempt to use a Fusarium subglutinans culture filtrate for the selection of pineapple cultivars resistant to fusariose disease

Borrás

Santos²,

Matos³

et al. 2001

Plant Breeding

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Pineapple, Ananas comosus L. Merr., cultivars differing in resistance to fusariose were examined for the phytotoxic effect of culture nitrate (CF), and fusaric acid (FA) isolated from Fusarium subglutinans (Wollew & Reinking) Nelson, Toussoun & Marasas. The possibility of using these substances on F1 hybrids as selection agents for resistance was also evaluated. The phytotoxic effect of these substances was assessed in pineapple plantlets from tissue culture by placing them on wounded leaf segments. The resistance level of the F1 hybrids and cultivars under natural conditions was measured in greenhouse field tests. The susceptible cultivars proved to be the most sensitive in each test, whereas resistant cultivars showed resistance to CF. FA affected all the cultivars independently of the degree of resistance of the cultivars The presence of other metabolites in CF was responsible for the screening effect between the varieties. Using CF as selection criteria allowed the selection of resistant plants with behaviour quite similar to plants where the fungus itself was used as the selection agent.

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Involvement of the Antioxidative System in Tomato Response to Fusaric Acid Treatment

Cited by 14 publications

References 0 publications

Effects of Fusaric Acid on Reactive Oxygen Species and Antioxidants in Tomato Cell Cultures

Effects of Fusaric Acid on Reactive Oxygen Species and Antioxidants in Tomato Cell Cultures

Catalase inhibition alters suberization and wound healing in potato (Solanum tuberosum) tubers

A first attempt to use a Fusarium subglutinans culture filtrate for the selection of pineapple cultivars resistant to fusariose disease

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