Involvement of the C-terminal Region in Phosphate Inhibition of Plasma Membrane Proton Pumping Activity

Abstract

Phosphate inhibited proton pumping across plasma membrane vesicles from rye leaves. Treatment with fusicaccin and trypsin however, which have been demonstrated to be effective in eliminating the autoinhibitory function of the Cterminal region of the plasma membrane proton pump, completely abolished the inhibition of proton pumping by phosphate. It is therefore suggested that phosphate inhibits the plasma membrane proton pumping activity in rye leaves under the influence of the C-terminal region.

Photosynthetic control of the plasma membrane H + -ATPase in Vallisneria leaves. II. Presence of putative isogenes and a protein equipped with a C-terminal autoinhibitory domain

“…The effects of Pi on the kinetic parameters of H + -transporting activity have been reported in rye leaves byKasai et al (2001), and published during the revision of this paper.…”