Involvement of the lncRNA AFAP1‐AS1/microRNA‐195/E2F3 axis in proliferation and migration of enteric neural crest stem cells of Hirschsprung's disease

Pan, Weikang; Wu, Ali; Yu, Hui; Yu, Qiang; Zheng, Bin; Yang, Weili; Tian, Donghao; Gao, Ya

doi:10.1113/ep088780

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…E2F3, E2F3a, and E2F3b exert synergistic or antagonistic roles in tumorigenesis and progression (18,19). Dysregulation of E2F3 expression is linked to diverse diseases, such as age-related cataracts (20) and Hirschsprung's disease (21). Additionally, E2F3 has been implicated in the development of diverse tumors as oncogenes.…”

Section: ' Discussionmentioning

confidence: 99%

CircularRNA_0119872 regulates the microRNA-582-3p/E2F transcription factor 3 pathway to promote the progression of malignant melanoma

Qu¹,

Yuan²,

Jia³

et al. 2021

Clinics

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Malignant melanoma (MM) is an invasive tumor that poses a threat to patient health. Circular RNAs (circRNAs) are important regulators of MM carcinogenesis. In this study, we investigated the expression characteristics and biological functions of, and mechanism underlying, circ_0119872 expression in MM. METHODS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to examine the circ_0119872, microRNA (miR)-582-3p, and E2F transcription factor 3 (E2F3) mRNA expression levels in MM tissues and cell lines. Western blotting was performed to quantify E2F3 protein expression. MM cells with circ_0119872 knockdown were established, and cell counting kit 8 (CCK-8) and transwell assays were utilized to examine the function of circ_0119872 and its effects on the malignant characteristics of MM cells. The MiRDB and TargetScan databases were used to predict the target genes of miR-582-3p. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to explore the biological functions of the target genes of miR-582-3p. Additionally, a dual-luciferase reporter gene experiment was performed to verify the targeting relationship between circ_0119872 and miR-582-3p as well as that between miR-582-3p and E2F3. RESULTS: Circ_0119872 was remarkably upregulated in MM tissues and cell lines. Circ_0119872 knockdown suppressed the cell proliferation and metastasis In addition, miR-582-3p was identified as a downstream target of circ_0119872. The target genes of miR-193a-3p are involved in melanogenesis and cancer-related signaling pathways. Mechanistically, circ_0119872 facilitated MM progression by adsorbing miR-582-3p and upregulating E2F3 expression. CONCLUSION: Circ_0119872 is an oncogenic circRNA that participates in the promotion of MM progression by regulating the miR-582-3p/E2F3 axis.

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Section: ' Discussionmentioning

confidence: 99%

CircularRNA_0119872 regulates the microRNA-582-3p/E2F transcription factor 3 pathway to promote the progression of malignant melanoma

Qu¹,

Yuan²,

Jia³

et al. 2021

Clinics

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“…For instance, lncRNA AFAP1-AS acts as a ceRNA by enhancing miR-181a expression through competitively binding, which in turn eventually block the ENCCs migration and invasion [ 66 ]. Further exploring this relationship, Pan et al [ 67 ] discovered that in HSCR patients' diseased intestinal sections, the reduced expression of lncRNA AFAP1-AS1 leads to decreased proliferation, migration, and invasive capacities of enteric neural crest stem cells (ENCSCs). hey also found that lncRNA AFAP1-AS1 can interact with miR-195 through the ceRNA mechanism, specifically regulating the expression of its target gene E2F3, thereby affecting the activity of ENCSCs.…”

Section: Non-coding Rna Classes Associated With Hscrmentioning

confidence: 99%

“…In HSCR, both miRNA-200a and lncRNA AFAP1-AS1 have been proven to be related to the occurrence of HSCR [ 29 , 67 ]. However, comprehensive research on the function of proteins or peptides encoded by miRNA-200a and lncRNA AFAP1-AS1 in HSCR is still scarce.…”

Section: Non-coding Rna Classes Associated With Hscrmentioning

confidence: 99%

The roles of non-coding RNAs in Hirschsprung's disease

Yang,

Hou,

Wang

et al. 2024

Non-coding RNA Research

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“…In addition, lncRNA DRAIC was found to regulate cell proliferation and migration in HSCR by affecting the miR-34a-5p/ITGA6 pathway ( 11 ). The silence of AFAP1-AS1 promoted HSCR progression by acting as an endogenous RNA to absorb miR-195 ( 12 ). Moreover, LOC100507600 is proved to participate in the development of HSCR through regulating BMI1 expression in a miR128-1-3p–dependent manner ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

LncRNA-RMST Functions as a Transcriptional Co-regulator of SOX2 to Regulate miR-1251 in the Progression of Hirschsprung's Disease

et al. 2022

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Hirschsprung's disease (HSCR) is a congenital disorder characterized by the absence of enteric neural crest cells (ENCCs). LncRNA rhabdomyosarcoma 2-associated transcript (RMST) is essential for the growth and development of neuron. This study aimed to reveal the role of RMST in the pathogenesis of HSCR. The expression level of RMST, miR-1251, SOX2, and AHNAK was evaluated with qRT-PCR or western blot. CCK-8 and transwell assays were applied to detect cell proliferation and migration. CHIP and RIP assays were applied to determine the combination relationship between SOX2 and promoter region of miR-1251 or RMST and SOX2, respectively. Dual-luciferase reporter assay was performed to confirm miR-1251 targeted AHNAK. As results have shown, RMST was downregulated in the aganglionic colon of HSCR patients. The knockdown of RMST attenuated cell proliferation and migration significantly. MiR-1251, the intronic miRNA of RMST, was also low expressed in HSCR, but RMST did not alter the expression of miR-1251 directly. Furthermore, SOX2 was found to regulate the expression of miR-1251 via binding to the promoter region of miR-1251, and RMST strengthened this function by interacting with SOX2. Moreover, AHNAK was the target gene of miR-1251, which was co-regulated by RMST and SOX2. In conclusion, our study demonstrated that RMST functioned as a transcriptional co-regulator of SOX2 to regulate miR-1251 and resulted in the upregulation of AHNAK, leading to the occurrence of HSCR. The novel RMST/SOX2/miR-1251/AHNAK axis provided potential targets for the diagnosis and treatment of HSCR during embryonic stage.

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Involvement of the lncRNA AFAP1‐AS1/microRNA‐195/E2F3 axis in proliferation and migration of enteric neural crest stem cells of Hirschsprung's disease

Cited by 10 publications

References 26 publications

CircularRNA_0119872 regulates the microRNA-582-3p/E2F transcription factor 3 pathway to promote the progression of malignant melanoma

CircularRNA_0119872 regulates the microRNA-582-3p/E2F transcription factor 3 pathway to promote the progression of malignant melanoma

The roles of non-coding RNAs in Hirschsprung's disease

LncRNA-RMST Functions as a Transcriptional Co-regulator of SOX2 to Regulate miR-1251 in the Progression of Hirschsprung's Disease

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