Involvement of the mitfa gene in the development of pigment cell in Japanese ornamental (Koi) carp (Cyprinus carpio L.)

Liu, J.H.; Wen, Sheng; Luo, Chen; Zhang, Y.Q.; Tao, Min; Wang, D.W.; Deng, Shuqing; Xiao, Yamei

doi:10.4238/2015.march.31.7

Cited by 18 publications

(6 citation statements)

References 37 publications

(39 reference statements)

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“…Koi carp, a colorful variety of common carp (Cyprinus carpio), is one of the popular ornamental fish spread worldwide that distinguished by coloration, patterning, and scalation (Damme et al, 2010). Extensive studies have been conducted on skin color regulation, including rearing environment (Jha and Barat, 2006), feed additives (Lin et al, 2011;Manas et al, 2017), genetics of pigment patterning (Wang and Li, 2004;Chistiakov and Voronova, 2009), gene functional analysis (Bar et al, 2013;Liu et al, 2015), and transcriptome analysis (Xu et al, 2014). Among these complicated regulation factors, genetics elements are the most important factors, thus the majority of gene resources related to skin color pigmentation need to be further elucidated.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.)

et al. 2020

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MicroRNAs (miRNAs) are~22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms of miRNAs to regulate skin color differentiation and pigmentation in fish have not been fully understood. Herein, we revealed that miR-206, a skin-enriched miRNA, regulates melanocortin 1 receptor (Mc1r, a key regulator of melanogenesis) expression by binding to its 3'-untranslated (UTR) region through bioinformatics and luciferase reporter assay in koi carp (Cyprinus carpio L.). The analysis of spatial and temporal expression patterns suggested that miR-206 is a potential regulator in the skin pigmentation process. Then, we silenced it in vivo with an antagomir method. The result showed a substantial increase of Mc1r mRNA expression and protein level, and also its downstream genes: tyrosinase (Tyr) and dopachrome tautomerase (Dct) that encoding key enzymes involved in melanin synthesis. Moreover, we constructed the miRNA-206 sponge lentivirus vector to transfect koi carp melanocytes in vitro, further checked the functions of melanocytes using Cck-8 and Transwell assays. As a result, inhibition of miR-206 significantly up-regulated Mc1r mRNA expression and protein level and accelerated the melanocyte proliferation and migration ability compared with the scrambled-sequence negative control group (miR-NC). Overall, these findings provide the evidence that miR-206 plays a regulatory role in the skin color pigmentation through targeting the Mc1r gene and would facilitate understanding the molecular regulatory mechanisms underlying miRNA-mediated skin color pigmentation in koi carp.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.)

et al. 2020

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“…At present, a mass of studies have been conducted on skin colour regulation of koi carp, such as inheritance of body colour (David et al., 2004; Gomelsky, Cherfas, & Hulata, 1998; Gomelsky, Cherfas, Hulata, & Dasgupta, 2003), functional analysis of skin pigment‐related genes (Bar, Kaddar, Velan, & David, 2013; Liu et al., 2015), feed additives (Maiti et al., 2017; Sun et al., 2012) and transcriptome analysis (Luo et al., 2018, 2019; Feng et al., 2018). In our previous study, we screened several miRNAs including ccr‐miR‐196a (hereinafter referred to as miR‐196a), which regulated pigmentation on different skins of koi carp by small RNA sequencing (Luo et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

“…At present, a mass of studies have been conducted on skin colour regulation of koi carp, such as inheritance of body colour (David et al, 2004;Gomelsky, Cherfas, & Hulata, 1998;Gomelsky, Cherfas, Hulata, & Dasgupta, 2003), functional analysis of skin pigment-related genes (Bar, Kaddar, Velan, & David, 2013;Liu et al, 2015), feed additives (Maiti et al, 2017;Sun et al, 2012) and transcriptome analysis (Luo et al, 2018(Luo et al, , 2019Feng et al, 2018).…”

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confidence: 99%

miR‐196a regulates the skin pigmentation of koi carp (Cyprinus carpioL.) by targeting transcription factormitfa

Yin

Luo

et al. 2020

Aquac. Res.

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Body colour of fish is an important phenotypic trait that closely related to its survival and reproduction, which involved in many biological processes such as mimicry, courtship, camouflage and environmental adaptation (Kelsh, 2004; Protas & Patel, 2008). Compared with mammals and birds, fish are ideal models for skin colour differentiation and pigmentation research, which possess various pigment cells including melanophores (black), xanthophores (yellow), erythrophores (red), iridophores (iridescent, blue, silver or gold) and leucophores (dull, whitish) (Braasch, Schartl, & Volff, 2007). Koi carp (Cyprinus carpio L.) is an ornamental fish selected from the common carp (C. carpio) with several colour variants including Kohaku, Taisho sanke and Showa sanshoku, thus provides good materials for the researches related to fish skin colour (De Kock & Gomelsky, 2015). To date, a series of genes have been identified in the differentiation and pigmentation in koi carp, such as melanogenesis pathway genes including tyrosinase (tyr), tyrosinase-related protein 1 (tyrp1), dopachrome tautomerase (dct), melanocortin 1 receptor (mc1r), agouti signalling protein (asip) and microphthalmia-associated transcription factor a (mitfa). Among them, mitfa is an important member of the mitf gene family that located in the centre of gene regulatory networks in which act as a 'master regulator', and its product has a basic/

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“…Skin color regulation in fish is a complicated process, linked to various cellular, genetic, nutritional, and environmental factors [16]. In Koi carp, much attention has been paid to skin color regulation, including the genetics of pigment patterning [17], cloning of color-related genes, functional analysis [18, 19], environmental factors [20], nutrition [21] and transcriptome analysis [22]. However, lncRNA-mediated regulatory mechanisms related to skin color have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of long non-coding RNA and mRNA expression in different colored skin of koi carp

et al. 2019

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Background: Long non-coding RNAs (lncRNAs) perform crucial roles in biological process involving complex mechanisms. However, information regarding their abundance, characteristics and potential functions linked to fish skin color is limited. Herein, Illumina sequencing and bioinformatics were conducted on black, white, and red skin of Koi carp (Cyprinus carpio L.). Results: A total of 590,415,050 clean reads, 446,614 putative transcripts, 4252 known and 72,907 novel lncRNAs were simultaneously obtained, including 92 significant differentially expressed lncRNAs and 722 mRNAs. Ccr_ lnc5622441 and Ccr_lnc765201 were up-regulated in black and red skin, Ccr_lnc14074601 and Ccr_lnc2382951 were up-regulated in white skin, and premelanosome protein a (Pmela), Pmelb and tyrosinase (Tyr) were up-regulated in black skin. The expression patterns of 18 randomly selected differentially expressed genes were validated using the quantitative real-time PCR method. Moreover, 70 lncRNAs acting on 107 target mRNAs in cis and 79 lncRNAs acting on 41,625 target mRNAs in trans were investigated. The resulting co-expression networks revealed that a single lncRNA can connect with numerous mRNAs, and vice versa. To further reveal their potential functions, Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed, and membrane, pigment cell development, cAMP signaling, melanogenesis and tyrosine metabolism appear to affect skin pigmentation. Additionally, three lncRNAs (Ccr_lnc142711, Ccr_lnc17214525 and Ccr_lnc14830101) and three mRNAs (Asip, Mitf and Tyr) involved in the melanogenesis pathway were investigated in terms of potential functions in embryogenesis and different tissues. Conclusions: The findings broaden our understanding of lncRNAs and skin color genetics, and provide new insight into the mechanisms underlying lncRNA-mediated pigmentation and differentiation in Koi carp.

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Involvement of the mitfa gene in the development of pigment cell in Japanese ornamental (Koi) carp (Cyprinus carpio L.)

Cited by 18 publications

References 37 publications

MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.)

MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.)

miR‐196a regulates the skin pigmentation of koi carp (Cyprinus carpioL.) by targeting transcription factormitfa

Integrated analysis of long non-coding RNA and mRNA expression in different colored skin of koi carp

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