Involvement of the Octadecanoid Pathway and Protein Phosphorylation in Fungal Elicitor-Induced Expression of Terpenoid Indole Alkaloid Biosynthetic Genes in <i>Catharanthus roseus</i>

Menke, Frank L.H.; Parchmann, Stefanie; Mueller, Martin J.; Kijne, Jan W.; Memelink, Johan

doi:10.1104/pp.119.4.1289

Cited by 220 publications

(171 citation statements)

References 43 publications

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“…Str, Dat, and G10h have been identified in a selected number of genes, which control metabolites synthesis involved in the biosynthesis pathway of TIAs (Rischer et al 2006). Fungal elicitors and methyl jasmonate (MeJa) had been reported to induce Str gene expression (Pasquali et al 1992;Menke et al 1999). The terminal step of vindoline biosynthesis is catalyzed by Dat.…”

Section: Discussionmentioning

confidence: 99%

The influence of different forms and concentrations of potassium nutrition on growth and alkaloid metabolism in Catharanthus roseus seedlings

Chang

Cong

Chen

et al. 2013

Journal of Plant Interactions

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In order to investigate the effect of potassium (K) nutrition on secondary metabolism, Catharanthus roseus was treated by different concentrations and forms of K nutrition (KNO 3 and K 2 SO 4 ). The results showed that the concentration of applied K obviously affected plant growth and biomass accumulation, while K form had little influence. The concentration of alkaloids, including vinblastine in the leaves and catharanthine in the leaves or roots, had a significant increase, especially upon 80 and 100 mM K nutrition. The measurement of internal ion content using energy dispersive spectroscopy displayed that the increase of K element in leaves was tightly correlated with elevated alkaloid concentration. The transcript levels of G10h, Str, and Dat genes, encoding key enzymes in the alkaloid biosynthesis pathway, were significantly activated by applying P and tightly related to alkaloid accumulation. In conclusion, our results provided the physiological and molecular evidence that potassium could effectively regulate alkaloid metabolism in C. roseus.

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Section: Discussionmentioning

confidence: 99%

The influence of different forms and concentrations of potassium nutrition on growth and alkaloid metabolism in Catharanthus roseus seedlings

Chang

Cong

Chen

et al. 2013

Journal of Plant Interactions

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“…For gene expression analysis, 5μg total RNA was extracted by the LiCl precipitation method (33) and was reverse transcribed and used for qRT PCR analysis (34) with SYBR green dye (SI Materials and Methods).…”

Section: Methodsmentioning

confidence: 99%

Enhancement of fruit shelf life by suppressingN-glycan processing enzymes

Meli

Ghosh²,

Prabha³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

200

167

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In a globalized economy, the control of fruit ripening is of strategic importance because excessive softening limits shelf life. Efforts have been made to reduce fruit softening in transgenic tomato through the suppression of genes encoding cell wall-degrading proteins. However, these have met with very limited success. N-glycans are reported to play an important role during fruit ripening, although the role of any particular enzyme is yet unknown. We have identified and targeted two ripening-specific N-glycoprotein modifying enzymes, α-mannosidase (α-Man) and β-D-N-acetylhexosaminidase (β-Hex). We show that their suppression enhances fruit shelf life, owing to the reduced rate of softening. Analysis of transgenic tomatoes revealed ≈2.5-and ≈2-fold firmer fruits in the α-Man and β-Hex RNAi lines, respectively, and ≈30 days of enhanced shelf life. Overexpression of α-Man or β-Hex resulted in excessive fruit softening. Expression of α-Man and β-Hex is induced by the ripening hormone ethylene and is modulated by a regulator of ripening, rin (ripening inhibitor). Furthermore, transcriptomic comparative studies demonstrate the downregulation of cell wall degradation-and ripening-related genes in RNAi fruits. It is evident from these results that N-glycan processing is involved in ripening-associated fruit softening. Genetic manipulation of N-glycan processing can be of strategic importance to enhance fruit shelf life, without any negative effect on phenotype, including yield.

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“…It does so by coordinately switching on the expression of multiple genes in the TIA biosynthetic pathway [5,20,21] and precursor pathways [5]. Therefore it was of interest to study the e¡ect of MeJA on G10h expression.…”

Section: Jasmonate-responsive Expression Of G10hmentioning

confidence: 99%

“…RNA extractions and Northern blot analysis were performed as described [20]. DNA probes for tryptophan decarboxylase (Tdc), strictosidine synthase (Str), strictosidine L-D-glucosidase (Sgd), 4OS ribosomal protein S9 (Rps9) [21] and Cpr [22] were described.…”

Section: Northern Blot Analysismentioning

confidence: 99%

Geraniol 10‐hydroxylase¹, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

Collu¹,

Unver²,

et al. 2001

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Geraniol 10-hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. Catharanthus roseus (Madagascar periwinkle) contains monoterpenoid indole alkaloids, several of which are pharmaceutically important. Vinblastine and vincristine, for example, find widespread use as anticancer drugs. G10H is thought to play a key regulatory role in terpenoid indole alkaloid biosynthesis. We purified G10H from C. roseus cells. Using degenerate PCR primers based on amino acid sequence information we cloned the corresponding cDNA. The encoded CYP76B6 protein has G10H activity when expressed in C. roseus and yeast cells. The stress hormone methyljasmonate strongly induced G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in a C. roseus cell culture. ß

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Involvement of the Octadecanoid Pathway and Protein Phosphorylation in Fungal Elicitor-Induced Expression of Terpenoid Indole Alkaloid Biosynthetic Genes in Catharanthus roseus

Cited by 220 publications

References 43 publications

The influence of different forms and concentrations of potassium nutrition on growth and alkaloid metabolism in Catharanthus roseus seedlings

The influence of different forms and concentrations of potassium nutrition on growth and alkaloid metabolism in Catharanthus roseus seedlings

Enhancement of fruit shelf life by suppressingN-glycan processing enzymes

Geraniol 10‐hydroxylase¹, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

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Involvement of the Octadecanoid Pathway and Protein Phosphorylation in Fungal Elicitor-Induced Expression of Terpenoid Indole Alkaloid Biosynthetic Genes in Catharanthus roseus

Cited by 220 publications

References 43 publications

The influence of different forms and concentrations of potassium nutrition on growth and alkaloid metabolism in Catharanthus roseus seedlings

The influence of different forms and concentrations of potassium nutrition on growth and alkaloid metabolism in Catharanthus roseus seedlings

Enhancement of fruit shelf life by suppressingN-glycan processing enzymes

Geraniol 10‐hydroxylase1, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

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Geraniol 10‐hydroxylase¹, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis