Involvement of the PKC–NF–κB signaling pathway in the regulation of T lymphocytes proliferation of chickens by conjugated linoleic acids

Abstract

The involvement of protein kinase C (PKC) and nuclear factor-κB (NF-κB) signaling pathway in immunoregulatory actions of conjugated linoleic acid (CLA) in chicken T lymphocyte was investigated. T lymphocytes in peripheral blood were isolated and cultured. The PKC and NF-κB inhibitors, staurosporine (SP) and pyrrolidine dithiocarbamate (PDTC), were used. SP inhibited the production of PKC, and PDTC partly blocked the NF-κB mRNA relative expression. Data suggested compared with c9, t11-CLA and t10, c12-CLA groups, the attenuated effects were showed in SP or PDTC treatment with the application of CLA, which suggested PKC and NF-κB mediated in the immunity of CLA. Furthermore, SP and PDTC inhibited the IL-2 secretion and T lymphocyte proliferation. SP or PDTC induced reduction of IL-2 secretion and T-lymphocytes proliferation were greatly or partially attenuated by CLA isomers. In conclusion, CLA was a potent immunomodulator for T lymphocytes through targeting the PKC-NF-κB pathway.

Immune activation effects of Eurotium cristatum on T cells through NF-κB signaling pathways in humans

“…NF-κB is an important transcription factor involved in the process of inflammation (Long et al, 2016). Activation of the NF-κB signaling pathway could adjust T cell subsets' percentage and result in T cell subsets' accumulation (Ma et al, 2014;Zhu, Pan, Yang, & Zhou, 2015).…”

“…The best known was the NF-κB signal pathway, which played a central role in the cellular response to a variety of stimuli, including cytokines such as IL-1 and TNFα. This pathway was important in the immune regulation (Long, Yang, Liu, Guo, & Wang, 2016;Zhang, Wang, Wang, & Zheng, 2013).…”