2010
DOI: 10.1016/j.fgb.2010.02.006
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Involvement of transposon-like elements in penicillin gene cluster regulation

Abstract: Subtelomeric secondary metabolite (SM) gene clusters are frequently surrounded by DNA repeats and transposon-like elements. The A. nidulans penicillin cluster, 30 kb from the telomere of chromosome VI, is bordered by such elements. Deletions of penicillin telomere proximal and distal border regions resulted in decreased penicillin production. A 3.7 kb distal region called PbIa, consisting of the putative transposable element DNA-2, was examined further where its replacement by a pyrG marker presented a similar… Show more

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Cited by 56 publications
(38 citation statements)
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“…Biosynthesis of this antibiotic is encoded by three genes (pcbAB, pcbC, penDE) which are clustered in the genome (van den . In Aspergillus nidulans, the penicillin cluster is located 30 kb from the telomere of chromosome VI and bordered by DNA repeats and transposon-like elements, and these elements regulate penicillin biosynthesis through interactions with putative chromatin remodeling complexes including the LaeA-Velvet or the HDAC complexes (Shaaban et al 2010). Deletion of a 3.7-kb distal region consisting of the putative transposable element DNA-2 in Aspergillus nidulans resulted in a decrease in penicillin gene expression and product formation.…”
Section: Secondary Metabolite Productionmentioning
confidence: 99%
“…Biosynthesis of this antibiotic is encoded by three genes (pcbAB, pcbC, penDE) which are clustered in the genome (van den . In Aspergillus nidulans, the penicillin cluster is located 30 kb from the telomere of chromosome VI and bordered by DNA repeats and transposon-like elements, and these elements regulate penicillin biosynthesis through interactions with putative chromatin remodeling complexes including the LaeA-Velvet or the HDAC complexes (Shaaban et al 2010). Deletion of a 3.7-kb distal region consisting of the putative transposable element DNA-2 in Aspergillus nidulans resulted in a decrease in penicillin gene expression and product formation.…”
Section: Secondary Metabolite Productionmentioning
confidence: 99%
“…In both cases, the genes are interspersed with large blocks of AT-rich repeats and are located near telomeres, but there are some differences in the repeats and, surprisingly, the active locus in Fl1 is much closer to the telomere than is the inactive locus in strain E2368. It may be informative to conduct a detailed analysis of the effects of certain sequences in these regions as enhancers or silencers, as has been done for penicillin genes in Aspergillus nidulans (Shaaban et al, 2010).…”
Section: Variations In Gene Expressionmentioning
confidence: 99%
“…Deleting portions of repetitive DNA surrounding the penicillin cluster in A. nidulans resulted in decreased gene expression and deregulation of the cluster. 90 This was true even in a sensitized genetic background lacking a histone deacetylase, where the genes were constitutively overexpressed. This suggests that the facultative heterochromatin consisting of repetitive elements surrounding gene clusters may serve as a guidance system for recruitment of machinery required for proper activation and transcription.…”
Section: Boundary Elementsmentioning
confidence: 99%