Involvement of twist in NNK exposure-promoted lung cancer cell migration and invasion

Wang, Yadong; Shi, Li; Li, Jiangmin; Li, Ang; Yang, Haiyan

doi:10.1016/j.tiv.2019.104740

Cited by 13 publications

(9 citation statements)

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“…The underlying mechanism involves the binding of Twist to the E-box element in the promoter region of the E-cadherin gene, which inhibits the activity of the promoter and leads to a reduction in the transcription of E-cadherin; this then induces EMT, which increases the migration, invasion and metastasis of tumor cells (15,16). Twist is known to play a role in a variety of invasive cancers, including breast (17), lung (18) and prostate cancer (19).…”

Section: Expression Of Twist Slug and Snail In Esophageal Squamous Cmentioning

confidence: 99%

“…The effects of Twist, Slug, Snail and clinicopathological parameters on the prognosis of esophageal cancer were also evaluated in the present study. Twist has been demonstrated to play an important role in a variety of invasive cancers, such as breast (17), lung (18) and prostate cancer (19). In one study, Twist was shown to be involved in nicotine-derived nitrosamine ketone-induced lung cancer cell migration and invasion (18).…”

Section: Os Pfs -----------------------------------------------------mentioning

confidence: 99%

“…Twist has been demonstrated to play an important role in a variety of invasive cancers, such as breast (17), lung (18) and prostate cancer (19). In one study, Twist was shown to be involved in nicotine-derived nitrosamine ketone-induced lung cancer cell migration and invasion (18). Lyu et al (19) demonstrated that Twist is a prognostic marker of prostate cancer, and identified potential downstream target and genes, which may be useful for predicting the prognosis of Twist-mediated prostate cancer.…”

Section: Os Pfs -----------------------------------------------------mentioning

confidence: 99%

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Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

Zhou

Biekemitoufu

et al. 2021

Oncol Lett

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Esophageal cancer is one of the most common types of malignancy worldwide. At present, surgical resection is the main treatment for esophageal cancer, but recurrence and distant metastasis are the main causes of mortality. The transcription factors Twist, Slug and Snail regulate epithelial-mesenchymal transition and thereby participate in tumor invasion and metastasis. The aim of the present study was to investigate the expression of Twist, Slug and Snail in esophageal squamous cell carcinoma (ESCC) and their prognostic significance. The expression of Twist, Slug and Snail in 229 paraffin-embedded ESCC and matched normal mucosal tissues was detected by immunohistochemistry. The expression differences of Twist, Slug and Snail in the ESCC and normal tissues were compared by χ 2 test, and the associations between the three proteins and the clinicopathological parameters of ESCC were analyzed. The expression levels of Twist, Slug and Snail in 29 fresh frozen ESCC and matched normal mucosal tissues were detected by reverse transcription-quantitative PCR. The correlations among Twist, Slug and Snail in ESCC were examined by Pearson's correlation analyses. In addition, single factor and multivariate Cox regression analyses were used to analyze the influence of Twist, Slug and Snail on the prognosis of ESCC. Twist was found to be highly expressed in ESCC. The difference of Slug expression in ESCC was associated with differentiation degree, TNM stage and vascular invasion, but no significant association was observed between Snail expression and any clinicopathological parameters. In ESCC, there were significant differences in protein expression between Twist and Snail, and Slug and Snail. The mRNA expression level of Twist in ESCC was significantly higher than that in normal esophageal mucosa. However, the mRNA expression of Slug in normal esophageal mucosa was higher than that in ESCC, and the mRNA expression levels of Twist and Snail were positively correlated in ESCC. Kaplan-Meier analysis of 229 patients with ESCC revealed that Snail influenced the overall survival, as did the co-expression of Twist and Snail. Nerve invasion was also identified as an independent factor affecting the progression-free survival of ESCC. The results indicate that Twist is highly expressed, Slug may be a tumor suppressor, and Snail is an independent prognostic factor in ESCC. Twist and Snail are positively correlated, and the simultaneous inhibition of Twist and Snail protein expression may be beneficial for prolonging the overall survival of patients with ESCC.

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Section: Expression Of Twist Slug and Snail In Esophageal Squamous Cmentioning

confidence: 99%

Section: Os Pfs -----------------------------------------------------mentioning

confidence: 99%

Section: Os Pfs -----------------------------------------------------mentioning

confidence: 99%

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Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

Zhou

Biekemitoufu

et al. 2021

Oncol Lett

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“…This kinase phosphorylates PKC and FAK creating a signaling loop that is activated by NNK, promoting cell migration and metastasis (53). Recently, Wang et al (54) observed that the exposure to NNK promoted migration and invasion of cancer cells through the increase in Twist protein levels, a transcription factor that had been previously associated with migration and invasion of different types of tumor cells. This transcription factor, in turn, increased N-cadherin mRNA expression, downregulated E-cadherin, a marker of epithelial cells, and therefore promoted EMT (54).…”

Section: Tobacco Smoke Contaminantsmentioning

confidence: 99%

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies

Albuquerque¹,

Manguinhas²,

Costa³

et al. 2021

Transl Lung Cancer Res

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Lung cancer (LC) is the leading cause of cancer deaths worldwide, being non-small lung cancer (NSCLC) sub-types the most prevalent. Since most LC cases are only detected during the last stage of the disease the high mortality rate is strongly associated with metastases. For this reason, the migratory and invasive capacity of these cancer cells as well as the mechanisms involved have long been studied to uncover novel strategies to prevent metastases and improve the patients' prognosis. This narrative review provides an overview of the main in vitro migration and invasion assays employed in NSCLC research. While several methods have been developed, experiments using conventional cell culture models prevailed, specifically the wound-healing and the transwell migration and invasion assays. Moreover, it is provided herewith a summary of the available information concerning chemical contaminants that may promote the migratory/ invasive properties of NSCLC cells in vitro, shedding some light on possible LC risk factors. Most of the reported agents with pro-migration/invasion effects derive from cigarette smoking [e.g., Benzo(a)pyrene and cadmium] and air pollution. This review further presents several studies in which different dietary/ plant-derived compounds demonstrated to impair migration/invasion processes in NSCLC cells in vitro.These chemicals that have been proposed as anti-migratory consisted mainly of natural bioactive substances, including polyphenols non-flavonoids, flavonoids, bibenzyls, terpenes, alkaloids, and steroids. Some of these compounds may eventually represent novel therapeutic strategies to be considered in the future to prevent metastasis formation in LC, which highlights the need for additional in vitro methodologies that more closely resemble the in vivo tumor microenvironment and cancer cell interactions. These studies along with adequate in vivo models should be further explored as proof of concept for the most promising compounds.

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“…Neste estudo foi realizado a exposição por quatro semanas em diferentes concentrações (0, 1, 2 e 5 µM) de NNK. O carcinógeno promoveu o aumento da expressão da proteína Twist e de seu RNA mensageiro, o que sugere que Twist pode estar envolvido na promoção de migração e invasão em células pulmonares provocado pelo NNK(WANG et al 2020). Entretanto, é prematuro afirmar que o gene Twist possui o mesmo papel em nosso modelo adotado de linhagem de hepatócitos murinos, contudo pode ser um caminho a ser melhor elucidado para o entendimento da promoção da migração e invasão neste tipo celular.Quando avaliamos viabilidade celular pela técnica do MTT em cultivo 2D e das células AML12 (CTL), pode-se observar um perfil de crescimento com curva ascendente ao longo das 72h em cada concentração celular.Já quando as células foram cultivadas em placas de baixa adesão (3D), o grupo controle não demonstrou um perfil de crescimento ao longo do tempo.Isso pode ser em decorreência de um processo chamado Anoikis, uma forma de morte programada induzida (apoptose) pela perda da adesão à matriz extracelular (PATANKAR et al 2019).Isso pode ter ocorrerido em virtude da falta de adesão e dependência de ancoragem, o que nos apresenta que esta célula do grupo contole não adquiriu propriedades tumorigênicas(LANDOLPH, 2014).O grupo que foi exposto ao carcinógeno, apresentou uma curva de crescimento crescente ao longo do tempo em cada concentração celular.…”

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Avaliação>i< in vitro>/i< dos efeitos da exposição crônica ao carcinógeno4-(methylnitrosamino)-1-(-3-pyridyl)-1-butanone(NNK)em hepatócitos murinos

Mizusaka¹

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Involvement of twist in NNK exposure-promoted lung cancer cell migration and invasion

Cited by 13 publications

References 50 publications

Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies

Avaliação>i< in vitro>/i< dos efeitos da exposição crônica ao carcinógeno4-(methylnitrosamino)-1-(-3-pyridyl)-1-butanone(NNK)em hepatócitos murinos

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