Involvement of Vesicle–Cytoskeleton Interaction in AQP5 Trafficking in AQP5-Gene-Transfected HSG Cells

Tada, J; Sawa, Takamasa; Yamanaka, Naoaki; Shono, Masayuki; Akamatsu, Tetsuya; Tsumura, Keio; Parvin, Most. Nahid; Kanamori, Norio; Hosoi, Kazuo

doi:10.1006/bbrc.1999.1828

Cited by 42 publications

(43 citation statements)

References 21 publications

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“…The signals which induce fusion are diverse and probably overlapping in some instances; a vasopressin-induced rise in cyclic AMP levels triggers protein kinase A-mediated phosphorylation of AQP2 and subsequent fusion of AQP2 vesicles to the plasma membrane (Kuwahara et al, 1995); a cyclic AMP-mediated event is also involved in fusion of AQP8-containing vesicles with the plasma membrane (Garcia et al, 2001). On the other hand, an acetylcholine-induced rise in Ca 2+ induces fusion of AQP5 vesicles, probably through a protein kinase C-mediated phosphorylation event (Ishikawa et al, 1998;Tada et al, 1999). Along these lines we demonstrated that the T. cruzi aquaporin is sequestered within the acidocalcisomal membrane and subsequently trafficked to the contractile vacuole following a cyclic AMP increase .…”

supporting

confidence: 62%

A contractile vacuole complex is involved in osmoregulation in Trypanosoma cruzi

Rohloff

Docampo

2008

Experimental Parasitology

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Acidocalcisomes are dense, acidic organelles with a high concentration of phosphorus present as pyrophosphate and polyphosphate complexed with calcium and other cations. Acidocalcisomes have been linked to the contractile vacuole complex in Chlamydomonas reinhardtii, Dictyostelium discoideum, and Trypanosoma cruzi. A microtubule-and cyclic AMP-mediated fusion of acidocalcisomes to the contractile vacuole complex in T. cruzi results in translocation of aquaporin and the resulting water movement which, in addition to swelling of acidocalcisomes, is responsible for the volume reversal not accounted for by efflux of osmolytes. Polyphosphate hydrolysis occurs during hyposmotic stress, probably increasing the osmotic pressure of the contractile vacuole and facilitating water movement. Index DescriptorsChlamydomonas reinhardtii; Dictyostelium discoideum; Trypanosoma cruzi; Kinetoplastida; acidocalcisomes; contractile vacuole Need for osmoregulation in Trypanosoma cruziTrypanosoma cruzi has a complex life cycle involving several morphological and functionally different stages. As T. cruzi progresses through its life cycle, it encounters diverse environmental stressors to which it must successfully adapt. Of particular interest is the parasite's ability to cope with extreme fluctuations in osmolarity that occur within the gut of the vector (Kollien et al., 2001;Kollien and Schaub, 2002) and also as the parasite moves from the insect gut through the acidic phagolysosome to the cytosol of the host cell. The infective form of the parasite passes out of the vector in the highly concentrated excreta (600-700 mOsm) (Kollien et al., 2001) and rapidly encounters the interstitial fluid of the mammalian host with a much lower osmolarity (300 mOsm). Clearly the parasite must have mechanisms that allow it to adapt both to hyperosmotic and hyposmotic stresses. In this review we limit our discussion to the response of T. cruzi to hyposmotic stress. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Adaptation to hyposmotic stress in vertebrate cellsPhysiological adaptations to hyposmotic stress have been studied extensively in a wide range of vertebrate cell types. Upon exposure to a reduction in external osmolarity, cells initially swell but soon regain nearly normal cell volume by a process that has been termed the Regulatory Volume Decrease (RVD; reviewed in (Lang et al., 1998a; Lang et al., 1998b), which is accomplished by the efflux of various inorganic ions (such as Na + and K + ) and organic osmolytes to the extracellular environment. Vertebrate cells maintain hig...

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supporting

confidence: 62%

A contractile vacuole complex is involved in osmoregulation in Trypanosoma cruzi

Rohloff

Docampo

2008

Experimental Parasitology

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“…Similarly to studies on AQPs 1 and 2, inhibitors of the microtubule network and actin microfilaments implicated cytoskeletal proteins in the mechanism of AQP5 trafficking to the plasma membrane (Tada et al, 1999). However, trafficking of AQP5 via microtubules (but not actin filaments) has been reported in Madin-Darby canine kidney cells (Karabasil et al, 2009).…”

Section: Cytoskeletal Proteinsmentioning

confidence: 88%

An emerging consensus on aquaporin translocation as a regulatory mechanism

Conner

Bill

Conner

2012

Molecular Membrane Biology

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Water can pass through the cell membrane relatively slowly by diffusion. However, in order to maintain water homeostasis, the rapid and specific regulation of cellular water flow is mediated by the aquaporin (AQP) family of membrane protein water channels. Thirteen human AQPs have been identified to date and the majority are highly specific for water while others show selectivity for water, glycerol and other small solutes. The wide range of tissues that are known to express AQPs is reflected by their involvement in many physiological processes and diseases. Receptor mediated translocation, via hormone activation, is an established method of AQP regulation, especially for AQP2. There is now an emerging consensus that the rapid and reversible translocation of other AQPs from intracellular vesicles to the plasma membrane, triggered by a range of stimuli, can confer increased membrane permeability. This review examines new advances that have identified molecular mechanisms of AQP regulation; these include the role of cytoskeletal proteins, kinases, calcium and retention or localisation signals as well as specific triggers of AQP translocation. This article reviews current knowledge of AQP regulation with a focus on rapid and dynamic regulation of sub-cellular AQP translocation in response to a specific trigger.3

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“…Trafficking of AQP5 is thought to be involved in the regulation of salivary water secretion via this channel protein ; e.g., in the parotid gland, AQP5 was reported to be trafficked from the intracellular vesicles to the apical membrane in response to stimulation by muscarinic receptors in vitro (15). In human salivary gland cells (HSG cells) transfected with AQP5 cDNA, the AQP5 protein is trafficked to the plasma membrane following an increase in [Ca 2+ ]i (17). All of these reports imply that trafficking of AQP5 from intracellular vesicles to the apical membrane is provoked by secretogogueinduced [Ca 2+ ]i increase leading to an increase in water permeability at the apical membrane.…”

Section: Discussionmentioning

confidence: 99%

“…For examination of the effects of inhibitors of PKA, microtubules, and microfilaments, cells cultured on polycarbonate membranes for 48 h were transfected with the chimeric gene, GFP-AQP5(wt) as described above and cultured under a growth-arrest condition (14), i.e., DMEM containing 0.5% FBS, for 18 h. The medium was then replaced with fresh medium containing 0.5% FBS and either 30 μM H-89 (14), 10 μM colchicine or 10 μM cytochalasin B (17) ; and the cells were cultured for another 6 h (14). During 6 h-culture, medium was replaced with fresh ones containing same inhibitors once at 3 h. The cells were then fixed, biotinylated, and examined under a confocal laser scanning microscope as described above.…”

Section: Treatment With H-89 Colchicine and Cytochalasin Bmentioning

confidence: 99%

Trafficking of GFP-AQP5 chimeric proteins conferred with unphosphorylated amino acids at their PKA-target motif (152SRRTS) in MDCK-II cells

et al. 2009

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Involvement of Vesicle–Cytoskeleton Interaction in AQP5 Trafficking in AQP5-Gene-Transfected HSG Cells

Cited by 42 publications

References 21 publications

A contractile vacuole complex is involved in osmoregulation in Trypanosoma cruzi

A contractile vacuole complex is involved in osmoregulation in Trypanosoma cruzi

An emerging consensus on aquaporin translocation as a regulatory mechanism

Trafficking of GFP-AQP5 chimeric proteins conferred with unphosphorylated amino acids at their PKA-target motif (152SRRTS) in MDCK-II cells

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