Involvement of Wnt4 signaling during myogenic proliferation and differentiation of skeletal muscle

Takata, Haruyuki; Terada, Kumiko; Oka, Hiroaki; Sunada, Yoshihide; Moriguchi, Takahiko; Nohno, Tsutomu

doi:10.1002/dvdy.21327

Cited by 53 publications

(58 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We investigated whether myostatin similarly regulates muscle fibroblast resistance to apoptosis in addition to inducing proliferation and ECM production. Dystrophic muscle fibroblasts were cultured with ActRIIBm.Fc, the protein synthesis inhibitor cycloheximide (CHX) or the topoisomerase II inhibitor etoposide (ET), two stimuli of programmed cell death (Cossu and Borello, 1999;Takata et al, 2007). Nearly all cultured dystrophic muscle fibroblasts underwent apoptosis following 12 hours of CHX or ET treatment as determined by expression of level of single stranded DNA ( Fig.…”

Section: Myostatin Regulates Proliferation and Ecm Production Of Dystmentioning

confidence: 99%

Inhibiting myostatin reverses muscle fibrosis through apoptosis

Zhang

Wagner

2012

Journal of Cell Science

View full text Add to dashboard Cite

SummarySkeletal muscle fibrosis is a defining feature of the muscular dystrophies in which contractile myofibers are replaced by fibroblasts, adipocytes and extracellular matrix. This maladaptive response of muscle to repetitive injury is progressive, self-perpetuating and thus far, has been considered irreversible. We have previously shown that myostatin, a known endogenous modulator of muscle growth, stimulates normal muscle fibroblasts to proliferate. Here, we demonstrate that myostatin also regulates the proliferation of dystrophic muscle fibroblasts, and increases resistance of fibroblasts to apoptosis through Smad and MAPK signaling. Inhibition of myostatin signaling pathways with a soluble activin IIB receptor (ActRIIB.Fc) reduces resistance of muscle fibroblasts to apoptosis in vitro. Systemic administration of ActRIIB.Fc in senescent mdx mice, a model of muscular dystrophy, significantly increases the number of muscle fibroblasts undergoing apoptosis. This leads to the reversal of pre-existing muscle fibrosis as determined by histological, biochemical and radiographical criteria. These results demonstrate that skeletal muscle fibrosis can be pharmacologically reversed through induction of fibroblast apoptosis.

show abstract

Section: Myostatin Regulates Proliferation and Ecm Production Of Dystmentioning

confidence: 99%

Inhibiting myostatin reverses muscle fibrosis through apoptosis

Zhang

Wagner

2012

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…In mammals, highly transforming Wnts have been associated to the canonical Wnt/ -catenin pathway, whereas non-transforming Wnts have been associated activate to the non-canonical pathway [4,6]. It is noteworthy that this binary classification is becoming obsolete, thus although Wnt4 was originally described as a non-canonical Wnt (nontransforming Wnt), it has also been implicated in the activation or inhibition of the canonical Wnt pathway [55,56,57,58,59,60,61]. Wnt7a has also been implicated in both canonical and non-canonical Wnt signaling depending on the cell and tissue context [62,63].…”

Section: Wnts the Ligandsmentioning

confidence: 99%

“…They found that Wnt4 treatment induced an increase in the muscle mass, particularly in fast-type muscle size. Furthermore, ectopic Wnt4 induces muscle satellite markers Pax7 and MyoD expression [60].…”

Section: Extrinsic Pathwaymentioning

confidence: 99%

“…Takata et al reported that Wnt4 exhibited a low but significant myogenic activity with an increase of proliferation rate in myoblast cells [60]. Conversely, Otto and co-workers found, using co-culture of Wnt4-producing NIH-3T3 cell line on single muscle fiber culture, a 24% reduction of satellite-cell proliferation [207].…”

Section: Wnt-related Regulation Of Myoblast Proliferation and Differementioning

confidence: 99%

“…Although this factor was originally described as a non-canonical Wnt, it has also been implicated in the activation or inhibition of the canonical Wnt pathway [55,56,57,58,59,60,61]. Numerous reports showed that Wnt-related myogenic activity is mediated mainly through a stimulation of canonical signaling.…”

Section: Wnt-related Regulation Of Myoblast Proliferation and Differementioning

confidence: 99%

See 2 more Smart Citations

Role and Function of Wnts in the Regulation of Myogenesis: When Wnt Meets Myostatin

Fédon¹,

Bonnieu²,

Vernus³

et al. 2012

Skeletal Muscle - From Myogenesis to Clinical Relations

View full text Add to dashboard Cite

Delta‐like noncanonical notch ligand 2 regulates the proliferation and differentiation of sheep myoblasts through the Wnt/β‐catenin signaling pathway

Sun,

Yuan,

et al. 2024

Journal Cellular Physiology

View full text Add to dashboard Cite

This study delved into the role of delta‐like noncanonical notch ligand 2 (DLK2) in the cell cycle, proliferation, apoptosis, and differentiation of myoblasts, as well as its interaction with the classical Wnt/β‐catenin signaling pathway in regulating myoblast function. The research revealed that upregulation of DLK2 in myoblasts during the proliferation phase enhanced myoblast proliferation, facilitated cell cycle progression, and reduced apoptosis. Conversely, downregulation of DLK2 expression using siRNA during the differentiation phase promoted myoblast hypertrophy and fusion, suppressed the expression of muscle fiber degradation factors, and expedited the differentiation process. DLK2 regulates myoblasts function by influencing the expression of various factors associated with the Wnt/β‐catenin signaling pathway, including CTNNB1, FZD1, FZD6, RSPO1, RSPO4, WNT4, WNT5A, and adenomatous polyposis coli. In essence, DLK2, with the involvement of the Wnt/β‐catenin signaling pathway, plays a crucial regulatory role in the cell cycle, proliferation, apoptosis, and differentiation of myoblasts.

show abstract

Involvement of Wnt4 signaling during myogenic proliferation and differentiation of skeletal muscle

Cited by 53 publications

References 82 publications

Inhibiting myostatin reverses muscle fibrosis through apoptosis

Inhibiting myostatin reverses muscle fibrosis through apoptosis

Role and Function of Wnts in the Regulation of Myogenesis: When Wnt Meets Myostatin

Delta‐like noncanonical notch ligand 2 regulates the proliferation and differentiation of sheep myoblasts through the Wnt/β‐catenin signaling pathway

Contact Info

Product

Resources

About