Involvement of β2-Adrenergic and μ-Opioid Receptors in Antinociception Produced by Intracerebroventricular Administration of α,β-Methylene-ATP

Fukui, Masato; Nakagawa, Takayuki; Minami, Masabumi; Satoh, Masamichi

doi:10.1254/jjp.86.423

Cited by 9 publications

(8 citation statements)

References 34 publications

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“…administration of P2X-receptor agonists produced antinociception (27) and the effect was attenuated by i.c.v. pretreatment with b 2 -adrenergic receptor antagonists in rats (28). In the present study, we found that pretreatment with the NA neurotoxin DSP-4 attenuated the antinociception by i.c.v.…”

Section: Discussionsupporting

confidence: 59%

“…a ,b-methylene-ATP was attenuated by i.c.v. pretreatment with b 2 -adrenergic receptor antagonists (28). These findings suggest that supraspinal P2X receptors play an inhibitory role in nociceptive transmission by activation of supraspinal b 2 -adrenergic receptors.…”

Section: Introductionmentioning

confidence: 70%

“…pretreatment with b 2 -, but not b 1 -, adrenergic receptor antagonists significantly attenuated the antinociception by i.c.v. a ,b -methylene-ATP (28). Taken together, it is suggested that supraspinally administered a ,b-methylene-ATP excites NA neurons arising from the LC through P2X receptors in the LC and induces the release of NA from the NA nerve terminals, and then the released NA acts on supraspinal b 2 -adrenergic receptors to produce antinociception.…”

Section: Discussionmentioning

confidence: 92%

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Involvement of Locus Coeruleus Noradrenergic Neurons in Supraspinal Antinociception by α,β-Methylene-ATP in Rats

et al. 2004

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Abstract. We reported previously that intracerebroventricular (i.c.v.) administration of P2X-receptor agonists produced antinociception and the effect was attenuated by i.c.v. pretreatment with b 2 -adrenergic receptor antagonists. The present study examined the involvement of noradrenergic neurons arising from the locus coeruleus (LC) in the supraspinal antinociception by the P2X-receptor agonist a ,b-methylene-ATP in rats. We found that pretreatment with DSP-4 (50 mg / kg, i.p.), which is a neurotoxin to selectively disrupt noradrenergic neurons arising from the LC, significantly attenuated the antinociception by i.c.v. administration of a ,b -methylene-ATP (10 nmol / rat). Microinjection of a ,b-methylene-ATP (0.1 and 1 nmol / side) into the bilateral LC significantly elevated the nociceptive threshold more potently than the i.c.v. administration at a dose of 10 nmol / rat. The antinociception by intra-LC injection of a ,b -methylene-ATP (1 nmol / side) was significantly attenuated by co-injection of pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (1 nmol / side), a non-selective P2X-receptor antagonist. These results suggest that noradrenergic neurons arising from the LC are involved in the supraspinal antinociception by a ,b -methylene-ATP through P2X receptors in the LC.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 92%

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Involvement of Locus Coeruleus Noradrenergic Neurons in Supraspinal Antinociception by α,β-Methylene-ATP in Rats

et al. 2004

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“…The antinociceptive effect of α,β-methylene-ATP administered i.c.v. was measured by the paw pressure test, as previously described [ 13 , 14 ]. Briefly, using an analgesimeter (Ugo Basile, Milan, Italy), the cuneate piston was put on the right hind paw and the pressure was loaded at a rate of 32 g/sec.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Role of Supraspinal P2X₃/P2X_2/3 Subtypes on Nociception in Rats

et al. 2006

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Extracellular ATP is known to mediate synaptic transmission as a neurotransmitter or a neuromodulator via ionotropic P2X and metabotropic P2Y receptors. Several lines of evidence have suggested that ATP facilitates pain transmission at peripheral and spinal sites via the P2X receptors, in which the P2X 3 subtype is considered as an important candidate for the effect. Conversely, we previously found that the activation of supraspinal P2X receptors evoked antinociception. However, the subtypes responsible for the antinociception via supraspinal P2X receptors remain unclear. In the present study, we showed that intracerebroventricular (i.c.v.) pretreatment with A-317491 (1 nmol), the novel non-nucleotide antagonist selective for P2X 3 and P2X 2/3 receptors, attenuated the antinociceptive effect produced by i.c.v. administered α,β-methylene-ATP (10 nmol), the P2X receptor agonist, in rats. Similarly, the abolishment of the P2X 3 receptor mRNA in the brainstem by repeated i.c.v. pretreatments with antisense oligodeoxynucleotide for P2X 3 gene once a day for 5 consecutive days diminished the antinociceptive effect of α,β-methylene-ATP. Furthermore, i.c.v. administration of A-317491 (1 and 10 nmol) significantly enhanced the inflammatory nociceptive behaviors induced by the intraplantar injection of formalin and intraperitoneal injection of acetic acid. Taken together, these results suggest that supraspinal P2X 3 /P2X 2/3 receptors play an inhibitory role in pain transmission. FindingsExtracellular ATP has been established as a signaling molecule that mediates diverse biological effects via P2 purinoceptors (P2X n and P2Y n ) in both the peripheral and central nervous systems [1,2]. A body of evidence indicates that ionotropic P2X receptors are involved in both peripheral and spinal pain transmission [3][4][5]. Of the seven P2X receptors identified to date, the expression of the P2X 3 receptors appear selective for a subpopulation of small-diameter dorsal root ganglion neurons, which are probably associated with nociception [6][7][8]. In vivo studies have provided evidence that the activation of P2X receptors, especially the P2X 3 subtype and P2X 2/3 heteromer, contributes to an acute nociceptive behavior, hyperalgesia and allodynia [9][10][11][12]. These observations support the idea that the P2X 3 and P2X 2/3 receptors play a crucial

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“…Naloxone is a non‐specific opioid receptor antagonist that has been demonstrated to reverse not only the effects of narcotics, but the analgesia associated with other drug classes as well, including tricyclic antidepressants, serotonin specific reuptake inhibitors, non‐steroidal anti‐inflammatory drugs, adenosine and alpha‐2 agonists (5–8). Despite the 7 h that elapsed since her last dose of narcotics, it is possible that the administration of naloxone resulted in the arousal of our patient directly because of its antagonism of hydromorphone.…”

mentioning

confidence: 99%

Is the analgesic effect of systemic lidocaine mediated through opioid receptors?

Cohen

Mao

2003

Acta Anaesthesiol Scand

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Involvement of β2-Adrenergic and μ-Opioid Receptors in Antinociception Produced by Intracerebroventricular Administration of α,β-Methylene-ATP

Cited by 9 publications

References 34 publications

Involvement of Locus Coeruleus Noradrenergic Neurons in Supraspinal Antinociception by α,β-Methylene-ATP in Rats

Involvement of Locus Coeruleus Noradrenergic Neurons in Supraspinal Antinociception by α,β-Methylene-ATP in Rats

Inhibitory Role of Supraspinal P2X₃/P2X_2/3 Subtypes on Nociception in Rats

Is the analgesic effect of systemic lidocaine mediated through opioid receptors?

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Involvement of β2-Adrenergic and μ-Opioid Receptors in Antinociception Produced by Intracerebroventricular Administration of α,β-Methylene-ATP

Cited by 9 publications

References 34 publications

Involvement of Locus Coeruleus Noradrenergic Neurons in Supraspinal Antinociception by α,β-Methylene-ATP in Rats

Involvement of Locus Coeruleus Noradrenergic Neurons in Supraspinal Antinociception by α,β-Methylene-ATP in Rats

Inhibitory Role of Supraspinal P2X3/P2X2/3 Subtypes on Nociception in Rats

Is the analgesic effect of systemic lidocaine mediated through opioid receptors?

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Inhibitory Role of Supraspinal P2X₃/P2X_2/3 Subtypes on Nociception in Rats