Inward Budding and Endocytosis of Membranes Regulated by de Novo Designed Peptides

Yu, Qiuhong; Sun, Jianbo; Huang, Siqi; Chang, Haojing; Bai, Qingwen; Chen, Yong-Xiang; Liang, Dehai

doi:10.1021/acs.langmuir.8b00882

Cited by 9 publications

(10 citation statements)

References 65 publications

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“…At the same time, deep insertion may induce negative curvature (Figure S1). Membrane deformation by electrostatic wrapping, and the neutralization of negatively charged lipids has also been reported as additional factors to induce negative curvature in a model membrane system. ,, We, therefore, hypothesized that invagination of cell membranes (plasma membranes) could be promoted by adding amphiphilic peptides having a negative-curvature propensity to living cells, resulting in the modulation of endocytosis and other cellular functions (Figure ). Endocytosis is a fundamental process in all eukaryotic cells by which extracellular materials are taken up by the invagination of the membrane to form encapsulating vesicles .…”

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confidence: 99%

An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature

et al. 2020

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Membrane curvature plays a pivotal role in cellular life, including cellular uptake and membrane trafficking. The modulation of membrane curvature provides a novel means of manipulating cellular events. In this report, we show that a nine-residue amphiphilic peptide (R6W3) stimulates endocytic uptake by inducing membrane curvature. Curvature formation on cell membranes was confirmed by observing the cellular distribution of the curvature-sensing protein amphiphysin fused with a yellow fluorescent protein (Amp-YFP). Dot-like signals of Amp-YFP were visible following the addition of R6W3, suggesting curvature formation in cell membranes, leading to endocytic cup and vesicle formation. The promotion of endocytic uptake was confirmed using the endocytosis marker polydextran. Treatment of cells with R6W3 yielded a 4-fold dextran uptake compared with untreated cells. The amphiphilic helical structure of R6W3 was also crucial for R6W3-stimulated endocytic uptake.

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confidence: 99%

An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature

et al. 2020

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“…27 Moreover, a similar finding was elucidated by Yu et al, where they showed that no inward budding induced by the peptide K 3 L 8 K 3 took place in DOPG/ DOPC/DPPC GUVs when cholesterol is absent. 43 The authors further emphasize the contribution of hydrophobic interactions between the peptides and the vesicles to the observed deformations. Nevertheless, obtaining such information is not inherent in vesicular systems.…”

Section: Morphology Deformations Of Dopc/chol Guvsmentioning

confidence: 91%

Effects of Bisphenol A on DOPC/Cholesterol Binary Mixtures: Implications for Cell Membrane Integrity

Lessa,

Ruiz,

Alessio

et al. 2024

ACS Appl. Nano Mater.

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This study explored the effects of bisphenol A (BPA), a residual contaminant derived from plastic materials, on binary mixtures of the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol (Chol). These mixtures were structured into bilayers, forming giant unilamellar vesicles (GUVs), and monolayers, forming Langmuir films, whose compositions and phases are relevant for mimicking the cell plasma membrane in eukaryotic cells. The composition of the binary mixture of DOPC/Chol 8:2, representing the proportion of the primary lipid groups found in cells, was effective in inducing inward buddings when exposed to BPA, in addition to the multiple shape transformations observed for DOPC vesicles. In monolayers, BPA induced the π–A isotherm expansion of DOPC, Chol, and the binary mixtures, also mainly in the DOPC/Chol 8:2 monolayers. Consistently, polarization-modulated infrared reflection absorption spectroscopy measurements revealed more pronounced interactions between BPA and the DOPC headgroup (hydrogen bonding from the hydrogen of the BPA phenol group and the nitrogen from the DOPC choline group, preferentially) in the DOPC/Chol 8:2 monolayer compared to that in individual DOPC monolayer. The presence of Chol appears to make the DOPC headgroups more accessible for interaction, even in bilayers. These results emphasize the substantial impact of BPA on the DOPC/Chol binary mixture, highlighting its critical role in predicting the potential damage to human cell membranes.

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“…Then, nanoparticles were incubated with cells at a final nanoparticle concentration of 100 µg mL −1 . Both CIS@M-F and CIS@M-C were labeled with Rhodamine B following a published protocol [ 65 ]. Following either 2 or 6 h of incubation, cells were harvested for flow cytometer analysis, and the MFI was recorded.…”

Section: Methodsmentioning

confidence: 99%

Radiodynamic therapy with CsI(na)@MgO nanoparticles and 5-aminolevulinic acid

et al. 2022

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Background Radiodynamic therapy (RDT) holds the potential to overcome the shallow tissue penetration issue associated with conventional photodynamic therapy (PDT). To this end, complex and sometimes toxic scintillator–photosensitizer nanoconjugates are often used, posing barriers for large-scale manufacturing and regulatory approval. Methods Herein, we report a streamlined RDT strategy based on CsI(Na)@MgO nanoparticles and 5-aminolevulinic acid (5-ALA). 5-ALA is a clinically approved photosensitizer, converted to protoporphyrin IX (PpIX) in cancer cells’ mitochondria. CsI(Na)@MgO nanoparticles produce strong ~ 410 nm X-ray luminescence, which matches the Soret band of PpIX. We hypothesize that the CsI(Na)@MgO-and-5-ALA combination can mediate RDT wherein mitochondria-targeted PDT synergizes with DNA-targeted irradiation for efficient cancer cell killing. Because scintillator nanoparticles and photosensitizer are administered separately, the approach forgoes issues such as self-quenching or uncontrolled release of photosensitizers. Results When tested in vitro with 4T1 cells, the CsI(Na)@MgO and 5-ALA combination elevated radiation-induced reactive oxygen species (ROS), enhancing damages to mitochondria, DNA, and lipids, eventually reducing cell proliferation and clonogenicity. When tested in vivo in 4T1 models, RDT with the CsI(Na)@MgO and 5-ALA combination significantly improved tumor suppression and animal survival relative to radiation therapy (RT) alone. After treatment, the scintillator nanoparticles, made of low-toxic alkali and halide elements, were efficiently excreted, causing no detectable harm to the hosts. Conclusions Our studies show that separately administering CsI(Na)@MgO nanoparticles and 5-ALA represents a safe and streamlined RDT approach with potential in clinical translation. Graphical Abstract

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Inward Budding and Endocytosis of Membranes Regulated by de Novo Designed Peptides

Cited by 9 publications

References 65 publications

An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature

An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature

Effects of Bisphenol A on DOPC/Cholesterol Binary Mixtures: Implications for Cell Membrane Integrity

Radiodynamic therapy with CsI(na)@MgO nanoparticles and 5-aminolevulinic acid

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