Iodine adsorption characteristics of newly-formed rat liver glycogen

Chapman, Douglas; Felts, J.M.; Chaikoff, I.L.

doi:10.1007/bf02161260

Cited by 4 publications

(1 citation statement)

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“…It is of interest that Chapman et al 41 found that the AM with added iodine almost doubled for liver glycogen isolated from diabetic rats after treatment with insulin. Also, they observed a doubling of the AM after 18-hr fasted rats were given glucose by stomach tube followed by ad libitum feeding.…”

Section: Discussionmentioning

confidence: 98%

Variation in the degree of branching of the glycogens and an approximate relationship between average exterior chain length and iodine staining

Fales

1980

Biopolymers

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SynopsisPrecise evaluation of the branching characteristics of glycogen complex from a variety of sources was made by replicate determinations of end groups by a reproducible periodate oxidation method and by replicate determinations of the percentage degradation by exhaustive 0-amylase activity. Also, the iodine staining characteristics of these samples were studied. The following observations were made. During the synthesis of glycogen by baker's yeast. there was a significant increase in the average chain length of the glycogen, and the maximal optical absorbance AM of the glycogen-iodine complex doubled. The AM decreased linearly with increasing temperature, and increases in iodine concentration caused increases in both the AM and the wavelength of maximal absorbance. There was a general increase in the AM of the glycogen with increasing exterior chain length, hut there were minor deviations due to some dependence on the source of the glycogen. Unlike the linear relationship between the AM per chain and the average chain length of the amyloses, the A M per chain was exponentially related to the average exterior chain length of the glycogens.

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Section: Discussionmentioning

confidence: 98%