Iododeoxyuridine administered to mice is de-iodinated and incorporated into DNA primarily as thymidylate

Commerford, S. L.; Joel, D.D.

doi:10.1016/0006-291x(79)90388-7

Cited by 23 publications

(2 citation statements)

References 4 publications

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“…Ser., in press). However, this enzymatic reaction has been shown to be irreversible with respect to dTMP demethylation under laboratory conditions (2). Additionally, the inability of the dihydrofolate reductase inhibitors amethopterin and trimethoprim to reduce nonspecific labeling (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of 5-Fluoro-2′-Deoxyuridine on [ ³ H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Jeffrey

Paul

1988

Appl Environ Microbiol

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The effect of 5-fluoro-2'-deoxyuridine (FdUrd) on [methyl-3H] thymidine incorporation by bacterioplankton populations in subtropical freshwater, estuarine, and oceanic environments was examined. In estuarine waters, intracellular isotope dilution was inhibited by FdUrd, which enabled us to estimate both intracellular and extraceliular isotope dilution. In 2 of 10 cases, extracellular isotope dilution was significant. At low concentrations of [methyl-3H]thymidine or [6-3H]thymidine, FdUrd completely inhibited incorporation of radioactivity into protein and RNA. At high concentrations of [3H]thymidine, however, FdUrd had little effect on labeling patterns. The dihydrofolate reductase inhibitors amethopterin and trimethoprim had no effect on macromolecular labeling patterns. These results suggest that thymidylate synthase is not involved in nonspecific labeling and that FdUrd inhibits nonspecific labeling by blocking some other enzyme involved in thymidine catabolism. In oligotrophic oceanic and freshwater samples, FdUrd did not inhibit intracellular isotope dilution or [3H]thymidine labeling of protein and RNA, but caused some inhibition of [3H]thymidine incorporation into DNA. The ability of FdUrd to inhibit nonspecific macromolecular labeling during (3H]thymidine incorporation was significantly correlated (r = 0.84) with total thymidine incorporation (in picomoles per liter per hour). The results are discussed in terms of applications of FdUrd to routine bacterial production measurements and the general assumptions of [3lHlthymidine incorporation.

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Section: Discussionmentioning

confidence: 99%

Effect of 5-Fluoro-2′-Deoxyuridine on [ ³ H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Jeffrey

Paul

1988

Appl Environ Microbiol

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“…As m5IUdR is hardly reutilized [4,6,13,14], the time-dependent loss of radioactivity in hematopoietic cells reflects cell turnover.…”

Section: Discussionmentioning

confidence: 99%

Proliferative compensation of residual radiation damage in the compartment of hematopoietic early progenitor cells of the mouse

Hübner

Wangenheim

Feinendegen

1984

Radiat Environ Biophys

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The rate of cell entry from the compartment of hematopoietic early progenitor cells into differentiation was determined in sublethally irradiated mice. By use of the criterion of repopulating ability, transplantation of 5-(125I) iodo-2'-deoxyuridine labeled bone marrow cells into fatally irradiated syngeneic recipients allows to measure the relative number of early progenitor cells lodging in the spleen and the turnover of these cells in the donors. Following 450 rad the relative number of transplantable early progenitor cells in S-phase recovers to normal within 2 weeks and stabilizes after 5 weeks. At this time, the labeled progenitors turn over with a half-time of 1.4-2.2 days; the respective times for unirradiated mice are 1.5-1.8 days. Thus, quantitative and qualitative residual radiation damage that is known to exist in the compartment of CFU-S, is disguised within 2-5 weeks after irradiation by proliferative compensation in the entirety of early hemopoietic precursor cells which are here defined by their capacity of self renewal and delivery of differentiated cells and of seeding to spleens of lethally irradiated recipients.

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Brain DNA Changes During Learning

Reinis

1986

Topics in the Neurosciences

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Iododeoxyuridine administered to mice is de-iodinated and incorporated into DNA primarily as thymidylate

Cited by 23 publications

References 4 publications

Effect of 5-Fluoro-2′-Deoxyuridine on [ ³ H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Effect of 5-Fluoro-2′-Deoxyuridine on [ ³ H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Proliferative compensation of residual radiation damage in the compartment of hematopoietic early progenitor cells of the mouse

Brain DNA Changes During Learning

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Iododeoxyuridine administered to mice is de-iodinated and incorporated into DNA primarily as thymidylate

Cited by 23 publications

References 4 publications

Effect of 5-Fluoro-2′-Deoxyuridine on [ 3 H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Effect of 5-Fluoro-2′-Deoxyuridine on [ 3 H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Proliferative compensation of residual radiation damage in the compartment of hematopoietic early progenitor cells of the mouse

Brain DNA Changes During Learning

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Effect of 5-Fluoro-2′-Deoxyuridine on [ ³ H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida

Effect of 5-Fluoro-2′-Deoxyuridine on [ ³ H]Thymidine Incorporation by Bacterioplankton in the Waters of Southwest Florida