Iohexol assay for direct determination of glomerular filtration rate: optimization and development of an HPLC-UV method for measurement in serum and urine

Assri, Soufiane El; Sam, Hicham; Assri, Ayoub El; Bentata, Y.; Saalaoui, Ennouamane; Rochdi, Chaymae; Sebbar, El-houcine; Choukri, Mohammed

doi:10.1016/j.cca.2020.04.038

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…According to a Newman et al research, Cystatin C is a more sensitive indication for GFR alterations, even if they are small. 14 The presence of Cystatin C in maternal blood was shown to be a quality signal for early identification of preeclampsia in research by Stevens et al 15 In Nirmala et al's research, BMI was 28 as well, while in ours, the mean BMI was 25. 16.…”

Section: Discussionmentioning

confidence: 50%

Correlation of Serum Cystatin-C and Creatinine as Predictive Prognostic Markers for Renal Impairment in Pre Eclamptic Patients

Shaikh¹,

Shaikh²,

Memon³

et al. 2021

PJMHS

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Background: Renal impairment is becoming more common as adverse effect of preeclampsia in emerging nations. Preeclampsia is a complication of pregnancy that can be prevented by using Cystatin C as a better and earlier renal biomarker than creatinine in order to evaluate the renal function. Method: The study was done on 150 women in the Obstetrics and Gynecology Department at LUMHS Jamshoro, dividing them into three gestational age groups. The ANNOVA test was used to determine if cystatin-c is a superior marker to serum creatinine. SPSS version 21 was used to analyze the data without any dropout of the patients. Results: In this study, one hundred fifty (150) pre eclamptic pregnant women in the 20–35-year-old age range were chosen. The cystatin-c level was 1.2 ± 0.7 at 36th weeks of gestation in the forty of the patients of age 20-25, while creatinine was 1 ± 0.7. In sixty-eight (68) patients of age between 25-30 years, cystatin-c levels ranged from 1.6 ± 0.8 ng/mL, while creatinine levels ranged from 1.1 ± 0.9 ng/mL. In patients above > 30 years’ age group, the serum cystatin C level was found to be 1.9 ± 0.8, while the creatinine level was found to be 1.2±0.6 at 36th week of gestation. Conclusion: This research shows that cystatin C levels rise early and proved to be a better predictor of renal problems as compared to serum creatinine, which didn't increase considerably. Keywords: Cystatin-C, Preeclampsia, kidney impairment

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Section: Discussionmentioning

confidence: 50%

Correlation of Serum Cystatin-C and Creatinine as Predictive Prognostic Markers for Renal Impairment in Pre Eclamptic Patients

Shaikh¹,

Shaikh²,

Memon³

et al. 2021

PJMHS

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“…46 There is no ultimate determinant of glomerular function, although iohexol, a radiographic contrast medium, fulfills several requirements of an ideal GFR marker. [47][48][49] Inulin or 51Cr-EDTA have limited value in measuring GFR in ICU patients, 44,50,51 whose kinetics of GFR may change rapidly 52,53 and hereby contribute to drug-induced toxicity, which also may be influenced by pharmacokinetics, compartmental dispersion, metabolism, and elimination. Hypoalbuminemia increases the free fraction of several antibiotics in plasma and hereby its elimination when cleared via the renal route.…”

Section: Discussionmentioning

confidence: 99%

Estimated glomerular filtration rates are higher when creatinine‐based equations are compared with a cystatin C‐based equation in coronavirus disease 2019

Larsson

Hultström

Frithiof

et al. 2022

Acta Anaesthesiol Scand

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Objectives: Estimations of glomerular filtration rate (eGFR) are based on analyses of creatinine and cystatin C, respectively. Coronavirus disease 2019 (COVID-19) patients in the intensive care unit (ICU) often have acute kidney injury (AKI) and are at increased risk of drug-induced kidney injury. The aim of this study was to compare creatinine-based eGFR equations to cystatin C-based eGFR in ICU patients with COVID-19. Methods: After informed consent, we included 370 adult ICU patients with COVID-19. Creatinine and cystatin C were analyzed at admission to the ICU as part of the routine care. Creatinine-based eGFR (ml/min) was calculated using the following equations, developed in chronological order; the Cockcroft-Gault (C-G), Modified Diet in Renal Disease (MDRD)1999, MDRD 2006, Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI), and Lund-Malmö revised (LMR) equations, which were compared with eGFR calculated using the cystatin C-based Caucasian Asian Pediatric Adult (CAPA) equation. Results:The median eGFR when determined by C-G was 99 ml/min and interquartile range (IQR: 67 ml/min). Corresponding estimations for MDRD1999 were 90 ml/min (IQR: 54); MDRD2006: 85 ml/min (IQR: 51); CKD-EPI: 91 ml/min (IQR: 47); and for LMR 83 ml/min (IQR: 41). eGFR was calculated using cystatin C and the CAPA equation value was 70 ml/min (IQR: 38). All differences between creatinine-based eGFR versus cystatin C-based eGFR were significant (p < .00001).Conclusions: Estimation of GFR based on various analyses of creatinine are higher when compared with a cystatin C-based equation. The C-G equation had the worst performance and should not be used in combination with modern creatinine analysis methods for determination of drug dosage in COVID-19 patients.

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“…Йогексол -неионный низкоосмолярный йодсодержащий рентгеноконтрастный препарат. агент, может быть отличной альтернативой, поскольку он отвечает всем требованиям идеального маркера [44,45]. Клиренс йогексола может осуществляться либо клиренсом из плазмы, либо клиренсом с мочой [46,47].…”

Section: экзогенные маркерыunclassified

Modern markers for determining the functional ability of the kidneys in urological practice.

Kazachenko¹,

Voytko²,

Prosyannikov³

et al. 2023

ECU

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Introduction. Over the past decade, there has been a progressive increase in the number of patients with chronic kidney disease (CKD). Early diagnosis of renal insufficiency allows to prevent irreversible consequences leading to disability and significant medical and economic costs. Purpose: to analyze modern markers for determining the functional ability of the kidneys. Materials and methods. A search was carried out in PubMed and Library, according to the keywords creatinine, urea, glomerular filtration rate (GFR), clearance, formulas for calculating creatinine clearance, cystatin C, β2-microglobulin, β-bound protein, NGAL, KIM-1, chronic kidney disease, chronic renal failure, urolithiasis. We found 389 sources no older than 10 years (published after 2012) that were relevant to the topic of the review. 80 articles were selected directly for citation in the review. Results. GFR assessment and detection of albuminuria are the most effective methods, but biomarkers used to calculate GFR are subject to a number of factors and do not allow assessing kidney function in real time. For early prediction of acute renal injury, the most suitable biomarkers are considered to be: NGAL and KIM-1 and IL-18. Equations combining cystatin C and creatinine r work better than equations using only cystatin C or creatinine, especially in situations where it is necessary to confirm chronic kidney disease. The combination of creatinine, cystatin C and the ratio of urine albumin to creatinine improves the stratification of the risk of progression of kidney disease and mortality. The latest developments in biomarkers allow us to hope that in the near future the assessment of the functional ability of the kidneys will be carried out in real time and will become a general clinical practice of a modern doctor. Conclusion. Modern biomarkers of the functional ability of the kidneys allow not only to evaluate the function, but also to screen for diseases, conduct differential diagnosis, and evaluate the effectiveness of the treatment. The modern approach to the diagnosis of renal dysfunction should be based not only on its diagnosis, but also on determining the topic of nephron damage.

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Iohexol assay for direct determination of glomerular filtration rate: optimization and development of an HPLC-UV method for measurement in serum and urine

Cited by 8 publications

References 16 publications

Correlation of Serum Cystatin-C and Creatinine as Predictive Prognostic Markers for Renal Impairment in Pre Eclamptic Patients

Correlation of Serum Cystatin-C and Creatinine as Predictive Prognostic Markers for Renal Impairment in Pre Eclamptic Patients

Estimated glomerular filtration rates are higher when creatinine‐based equations are compared with a cystatin C‐based equation in coronavirus disease 2019

Modern markers for determining the functional ability of the kidneys in urological practice.

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