Ion currents in embryo development

Tosti, Elisabetta; Boni, Raffaele; Gallo, Alessandra

doi:10.1002/bdrc.21125

Cited by 21 publications

(10 citation statements)

References 129 publications

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“…These data suggest that the functions of ciGluA during early development may be independent from GluA's roles in synaptic transmission. Our identification of ascidian GluA suggests that a prototypical gluA gene encoded a calcium-permeable channel and may have been associated with calcium signaling involved during development (69,70). This gene likely later evolved into the four different gluA genes (GluA1, GluA2, GluA3, and GluA4) present in mammals that function to depolarize cells through mainly sodium influx.…”

Section: Discussionmentioning

confidence: 86%

AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate

Hirai

Hotta

Kubo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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AMPA-type glutamate receptors (GluAs) mediate fast excitatory transmission in the vertebrate central nervous system (CNS), and their function has been extensively studied in the mature mammalian brain. However, GluA expression begins very early in developing embryos, suggesting that they may also have unidentified developmental roles. Here, we identify developmental roles for GluAs in the ascidian Ciona intestinalis. Mammals express Ca2+-permeable GluAs (Ca-P GluAs) and Ca2+-impermeable GluAs (Ca-I GluAs) by combining subunits derived from four genes. In contrast, ascidians have a single gluA gene. Taking advantage of the simple genomic GluA organization in ascidians, we knocked down (KD) GluAs in Ciona and observed severe impairments in formation of the ocellus, a photoreceptive organ used during the swimming stage, and in resorption of the tail and body axis rotation during metamorphosis to the adult stage. These defects could be rescued by injection of KD-resistant GluAs. GluA KD phenotypes could also be reproduced by expressing a GluA mutant that dominantly inhibits glutamate-evoked currents. These results suggest that, in addition to their role in synaptic communication in mature animals, GluAs also have critical developmental functions.

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Section: Discussionmentioning

confidence: 86%

AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate

Hirai

Hotta

Kubo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Finally, membrane permeability was another term associated with many significant gene-sets implicated in pregnancy loss, including potassium channel, voltage dependent, EAG (IPR003949), positive regulation of potassium ion transmembrane transport (GO:1901381), glucose transporter type 1 (DO51272 ), regulation of glucose transmembrane transport (GO:0034765), regulation of ion transmembrane transport (GO:0034765), membrane potentials (D008564), potassium ion transmembrane transport (GO:0071805). Membrane permeability plays a crucial role in signal transduction, intercellular communication for exchange of gas and nutrients, and controls embryogenesis and growth 48 . Voltage gated potassium channels are also expressed in uterine smooth muscle and play a significant role in modulating uterine contractility during pregnancy 49 .…”

Section: Resultsmentioning

confidence: 99%

Genes and pathways associated with pregnancy loss in dairy cattle

Sigdel

Bisinotto

Peñagaricano

2021

Sci Rep

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Pregnancy loss directly impairs reproductive performance in dairy cattle. Here, we investigated genetic factors associated with pregnancy loss following detection of a viable embryo around 42 days of gestation. The objectives of this study were to perform whole-genome scans and subsequent gene-set analyses for identifying candidate genes, functional gene-sets and gene signaling pathways implicated in pregnancy loss in US Holstein cows. Data consisted of about 58,000 pregnancy/abortion records distributed over nulliparous, primiparous, and multiparous cows. Threshold models were used to assess the binary response of pregnancy loss. Whole‐genome scans identified at least seven genomic regions on BTA2, BTA10, BTA14, BTA16, BTA21, BTA24 and BTA29 associated with pregnancy loss in heifers and lactating cows. These regions harbor several candidate genes that are directly implicated in pregnancy maintenance and fetal growth, such as CHST14, IGF1R, IGF2, PSEN2, SLC2A5 and WNT4. Moreover, the enrichment analysis revealed at least seven significantly enriched processes, containing genes associated with pregnancy loss, including calcium signaling, cell–cell attachment, cellular proliferation, fetal development, immunity, membrane permeability, and steroid metabolism. Additionally, the pathway analysis revealed a number of significant gene signaling pathways that regulate placental development and fetal growth, including Wnt, Hedgehog, Notch, MAPK, Hippo, mTOR and TGFβ pathways. Overall, our findings contribute to a better understanding of the genetic and biological basis of pregnancy loss in dairy cattle and points out novel strategies for improving pregnancy maintenance via marker‐assisted breeding.

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“…Four genes, namely SCNN1A, KCNG3, KCNK5, and HTR3A, involved in the formation of ion channels can be found in our pluripotency critical gene list. Ion channels play a crucial role in signal transduction and its importance is well known in embryonic development (Tosti et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq analysis reveals pluripotency-associated genes and their interaction networks in human embryonic stem cells

Ghosh

Som

2020

Computational Biology and Chemistry

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Insight into the key genes of pluripotency in human and their interrelationships is necessary for understanding the underlying mechanism of pluripotency and hence their successful application in regenerative medicine. The recent advances in transcriptomics technologies have created new opportunities to decipher the genes involved in pluripotency, genetic network that governs the unique properties of embryonic stem cells and lineage differentiation mechanisms in a deeper scale. There are a large number of experimental studies on human embryonic stem cells (hESCs) being routinely conducted for unfolding the underlying biology of embryogenesis and their clinical prospects.However, the outcome of these studies often lacks consensus due to differences in samples, experimental techniques and/or analysis protocols. A universal stemness gene list is still lacking. In this quest, we compared transcriptomic profiles of pluripotent and non-pluripotent samples from diverse cell lines/types generated through RNA-sequencing (RNA-seq). We used a uniform pipeline for the analysis of raw RNA-seq data in order to reduce the amount of variation. Our analysis revealed a consensus set of 498 pluripotency-associated genes and 432 genes as potential pluripotent cell differentiation markers. Furthermore, we predicted 32 genes as "pluripotency critical genes".Reconstruction and analysis of co-expression networks further highlighted the importance of these genes. Gene ontology (GO) and pathway enrichment analysis, StemChecker and literature survey confirmed the involvement of the genes in the induction and maintenance of pluripotency, though more experimental studies are required for understanding their molecular mechanisms in human.

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Ion currents in embryo development

Cited by 21 publications

References 129 publications

AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate

AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate

Genes and pathways associated with pregnancy loss in dairy cattle

RNA-Seq analysis reveals pluripotency-associated genes and their interaction networks in human embryonic stem cells

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