Ion-driven communication and acclimation strategies in microalgae

Amaral, Raquel; Duci, Damiano; Cotta, Francisco C.; Bacellar, Felipe L.; Oliveira, Soraia; Verret, Frédéric; Asadi, Kamal; Vandamme, Lode K.J.; Reis, Nuno M.; Bryant, Lee D.; Tosh, David; Mouget, Jean-Luc; Perkins, Rupert; Rocha, Paulo R.F.

doi:10.1016/j.cej.2023.144985

Cited by 6 publications

(5 citation statements)

References 79 publications

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“…Amaral et al . introduced the term handover distance to describe the furthest distance cells can communicate with each other [ 23 ]. Population of Pseudo-nitzschia fraudulenta under darkness showed Ca 2+ -mediated quasi-periodic electrical oscillations, with a handover distance of 120 μm [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…introduced the term handover distance to describe the furthest distance cells can communicate with each other [ 23 ]. Population of Pseudo-nitzschia fraudulenta under darkness showed Ca 2+ -mediated quasi-periodic electrical oscillations, with a handover distance of 120 μm [ 23 ]. In this study, the three cell density gradients were 1.5 × 10 6 cells/ml, 3 × 10 6 cells/ml, and 6 × 10 6 cells/ml, corresponding to intercellular distances of 87.36 μm, 69.34 μm, and 55.03 μm, respectively, indicating that the handover distance of density signals is greater than 87.36 μm.…”

Section: Discussionmentioning

confidence: 99%

“…A very recent and novel hypothesis proposed that environment trigger could elevate intracellular Ca 2+ concentration, then most Ca 2+ are expelled out of cells via Ca 2+ pumps. The elevated extracellular Ca 2+ concentration formed Ca 2+ waves and reaches the neighboring cells, acting as an intercellular communication signal [ 23 ]. This may also lead to the phenomenon that the density signal cannot be completely blocked though PtSLC24A has been knocked out.…”

Section: Discussionmentioning

confidence: 99%

“…For example, exogenous N-acyl homoserine lactone, a signal molecules bacterial, could trigger cellular Ca 2+ efflux in diatoms [ 31 ]. In the studies and the latest reviews conducted by Rocha et al ., upon direct exposure to environmental stimuli such as infochemicals, microalgal cells can generate extracellular Ca 2+ waves, serving as a signal to transmit and amplify environmental information within the population [ 23 , 32 ]. Thus, for cells, the received signals may encompass both chemical signals and electrical signals based on Ca 2+ waves, with the latter also originating from chemical signals.…”

Section: Discussionmentioning

confidence: 99%

“…In the intracellular signaling process, some biotic-derived infochemicals, such as 2E, 4E/Z-decadienal (DD), and other bacterial quorum-sensing molecules, could induce the intracellular Ca 2+ signaling and NO responses [ 30–32 ]. Those fluctuated intracellular Ca 2+ signaling-induced extracellular bioelectricity is supposed to be a kind of paracrine signaling in microalgae communication [ 23 ]. Compared with the accumulated evidence regarding density-sensing mechanisms in bacteria, research in diatoms lags significantly behind because of the complexity of oceanic environment.…”

Section: Introductionmentioning

confidence: 99%

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SLC24A-mediated calcium exchange as an indispensable component of the diatom cell density-driven signaling pathway

Liu,

Zuo,

Xie

et al. 2024

The ISME Journal

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Diatom bloom is characterized by a rapid increase of population density. Perception of population density and physiological responses can significantly influence their survival strategies, subsequently impacting bloom fate. The population density itself can serve as a signal, which is perceived through chemical signals or chlorophyll fluorescence signals triggered by high cell density, and their intracellular signaling mechanisms remain to be elucidated. In this study, we focused on the model diatom, Phaeodactylum tricornutum, and designed an orthogonal experiment involving varying cell densities and light conditions, to stimulate the release of chemical signals and light-induced chlorophyll fluorescence signals. Utilizing RNA-Seq and Weighted Gene Co-expression Network Analysis, we identified four gene clusters displaying density-dependent expression patterns. Within these, a potential hub gene, PtSLC24A, encoding a Na+/Ca2+ exchanger, was identified. Based on molecular genetics, cellular physiology, computational structural biology and in situ oceanic data, we propose a potential intracellular signaling mechanism related to cell density in marine diatoms using Ca2+: Upon sensing population density signals mediated by chemical cues, the membrane-bound PtSLC24A facilitates the efflux of Ca2+ to maintain specific intracellular calcium levels, allowing the transduction of intracellular density signals, subsequently regulating physiological responses, including cell apoptosis, ultimately affecting algal blooms fate. These findings shed light on the calcium-mediated intracellular signaling mechanism of marine diatoms to changing population densities, and enhances our understanding of diatom bloom dynamics and their ecological implications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%