Ion Exchange as a Separations Method. IV. A Theoretical Analysis of the Column Separations Process¹

The Chromatography of Insulin on Deae-Cellulose in Buffers Containing 8m Urea

“…A theoretical distribution curve (Mayer and Tompkins 1947) agreed well with the experimental curve for the main peak (B) of insulin No. 2189 in Figure 1 which chromatographed with an Rp of 0·5.…”

“…The method of Mayer and Tompkins (1947) was used to calculate the theoretical elution curve using the parameters obtained with insulin on the DEAEcellulose column. For this purpose it was necessary to know the hold-up volume (volume of mobile liquid held between the swollen cellulose particles) of the column.…”

Simplified Design Model for an Ion Exclusion Column

“…The plate theory of chromatographic columns was proposed by Martin and Synge (3) and expanded by Mayer and Tompkins ( 4). Their model utilized a linear sorption isotherm and was based on a discontinuous equilibration process.…”

“…This approach formed the basis of the successful experiments described earlier on the development of porous cellulose acetate films capable of giving both high flow rates and high degrees of demineralization of aqueous sodium chloride solutions (9). The reported upswing (4) in the studies on the above separation technique for saline water conversion is largely a direct result of this approach.…”

Analytical separations using chelating ion exchange and partition chromatography

“…One of the most popular theories to explain the behavior of the chromatographic column is the plate theory which was first introduced by Martin and Synge (25) and expanded by later authors such as Mayer and Tompkins (26). In the theory of Martin and Synge, the column is thought of as containing discrete units called plates in which equilibration takes place somewhat analogous to an individual unit of a Craig countercurrent apparatus.…”