Ion Exchange Chromatography for Quantitative Analysis of Radioactive Histamine Metabolites in Human Urine

Bergmark, J.; Granérus, Göran

doi:10.3109/00365517409049893

Cited by 32 publications

(16 citation statements)

References 16 publications

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“…Certainly for cats, conclusions based on urine analysis as to the metabolism of ingested histamine were misleading since more than one metabolic route was involved. The same could apply for man, for although products of DAO and imidazole-N-methyl transferase activities are present in equal concentrations in the urine (Bergmark & Granerus, 1974;Sjaastad & Sjaastad, 1974; see Schayer, 1956 for a dissenting view), DAO may nevertheless predominantly inactivate dietary histamine; evidence for this could be obtained by analysing venous blood.…”

Section: Discussionmentioning

confidence: 99%

“…There are possible explanations for this discrepancy other than attribution to individual animal variation. First, in the present study histamine was instilled into the duodenum and urine collected for 2 h whereas Schayer (1956) gave histamine orally and collected urine over 6 h. Second, the isotope dilution technique (Schayer, 1956) probably underestimates the amount of imidazoleacetic acid riboside (Bergmark & Granerus, 1974;Sjaastad & Sjaastad, 1974), admittedly a minor metabolite in this study, and overestimates t-methylimidazoleacetic acid (Sjaastad & Sjaastad, 1974), thus exaggerating the importance of N-methylation. The differences between Schayer's and the present study could not be attributed to anaesthesia since results from spinal non-anaesthetized preparations and cats anaesthetized with chloralose were similar.…”

Section: Discussionmentioning

confidence: 99%

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Metabolism and Distribution of Exogenous Histamine in Cats

Imrie¹,

Marley²,

Thomas³

1978

British J Pharmacology

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1 The metabolism and disposition in blood and tissues of exogenous ["4C]-histamine was examined in cats.2 The principal metabolites in blood of histamine instilled into the small intestine (directly or by transfer from the stomach) and colon were imidazoleacetic acid and t-methylimidazoleacetic acid, being present in approximately equal amounts although in individual cats one or other acid could predominate. Only small amounts of histamine entered the circulation although in two of four cats given the largest dose (82 1imol/kg) large amounts were recovered. The amount of "4C radioactivity absorbed varied directly with the dose instilled. The chief metabolite in kidney and urine, whether histamine was instilled into the intestine or infused parenterally, was t-methylimidazoleacetic acid. Histamine was not absorbed from the stomach and its metabolism there was negligible. 3 In contrast, when histamine was infused into blood leaving the intestine (portal vein) the main metabolite in blood and tissues was t-methylimidazoleacetic acid being found in approximately 5-fold the concentration of imidazoleacetic acid. The small amount of histamine which eluded inactivation/uptake by liver, lungs, heart during the infusion was halved on circulation through the intestine. When histamine was infused into blood supplying the intestine, (cranial mesenteric artery) t-methylimidazoleacetic acid while still the major metabolite in blood was now only 1.4 times the concentration of imidazoleacetic acid. Additionally, the blood concentration of histamine during the infusion exceeded that of the metabolites. 4 t-Methylimidazoleacetic acid was also the principal metabolite in blood and tissues following histamine infusion into a cannula carrying a replacement venous blood supply to the liver of abdominally eviscerated cats. Imidazoleacetic acid and t-methylhistamine were present in equal concentrations and in one-quarter to one-third that of the methylated acid. The latter was also the principal metabolite following intra-arterial histamine infusion to abdominally eviscerated cats without a hepatic blood supply, although initially t-methylhistamine predominated: a large peak of histamine was present during the infusion period. When additionally the renal vessels were ligated, t-methylhistamine predominated throughout the experiment. 5 In conclusion, intraduodenally instilled histamine was metabolized equally by diamine oxidase and imidazole N-methyltransferase (followed by deamination by monoamine oxidase). In contrast, imidazole N-methyltransferase was the principal inactivator of parenterally infused histamine, deamination of t-methylhistamine by monoamine oxidase becoming progressively less efficient with the cumulative exclusion of the intestines, liver and kidney from the circulation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolism and Distribution of Exogenous Histamine in Cats

Imrie¹,

Marley²,

Thomas³

1978

British J Pharmacology

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“…HA is also deaminated by diamine oxidase (DAO), forming imidazoleacetaldehyde, which is converted to imidazoleacetic acid [8][9][10]. The pattern of labeled HA metabolites recovered in urine following slow infusion of small amounts of labeled HA into healthy humans suggests that, under these conditions, the contributions of each pathways to HA degradation are nearly equal [11]. Oral administration of 1 mg/kg of aminoguanidine (AG) (1 mg/kg) nearly abolished the amounts of oxidative metabolites, and enhanced the quantities of methylated metabolites and unmetabolized HA recovered in urine [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of aminoguanidine on pre- and post-irradiation regional cerebral blood flow, systemic blood pressure and plasma histamine levels in the primate

Cockerham¹,

Prell

Cerveny³

et al. 1991

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“…In eight control experiments radioactivity was measured in the leucine fractions following isolation on an Aminex 6 resin column at + 54°C as previously de scribed [1]. The radioactivity found in the leucine fractions was 96.2 ± 1.15% of total radioactivity with no difference between control and ischemia.…”

Section: Specific Radioactivity O F Precursor Amino Acid In Vitromentioning

confidence: 99%

Evaluation of an in vitro Method for the Study of Hepatic Protein Synthesis in Liver Ischemia

J¹,

Bergmark²,

Jagenburg

et al. 1985

Eur Surg Res

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The effects of liver ischemia on hepatic protein synthesis were studied by measuring the incorporation of radioactivity into protein in rat liver slices incubated in a medium containing ¹⁴C-leucine and a mixture of 19 L-amino acids at a concentration four times that in plasma. Following liver ischemia for 1 h the amount of radioactivity incorporated into protein was reduced by about 60%. Specific radioactivity of free ¹⁴C-leucine in incubation medium and incubated liver slices was not different when slices from control or ischemic liver were incubated. Thus, apparent reduction of leucine incorporation into protein in ischemic liver slices probably reflected decreased protein synthesis and not reduction of precursor specific radioactivity. Changes of in vitro protein synthesis in the postischemic liver (1 h following reperfusion) were compared to changes in vivo. Protein synthesis in vivo was determined from specific radioactivity of protein-bound and free ¹⁴C-leucine in liver tissue following intravenous administration of a large dose of ¹⁴C-leucine. Protein synthesis in vivo was reduced to 30% of the control value in the postischemic liver. The corresponding value in vitro was 40 %. Alterations of protein synthesis in vitro during and after a period of liver ischemia were similar to changes of ATP in liver tissue. Thus, changes of protein synthesis noticed in vitro reflected alterations of protein synthesis in vivo and changes of energy level in liver tissue. The results indicate that the present in vitro system is suitable for studying the effects of ischemia on hepatic protein synthesis.

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Ion Exchange Chromatography for Quantitative Analysis of Radioactive Histamine Metabolites in Human Urine

Cited by 32 publications

References 16 publications

Metabolism and Distribution of Exogenous Histamine in Cats

Metabolism and Distribution of Exogenous Histamine in Cats

Effects of aminoguanidine on pre- and post-irradiation regional cerebral blood flow, systemic blood pressure and plasma histamine levels in the primate

Evaluation of an in vitro Method for the Study of Hepatic Protein Synthesis in Liver Ischemia

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