Ion-exchange electrokinetic chromatography with polymer ions for the separation of isomeric ions having identical electrophoretic mobilities

Terabe, Shigeru; Isemura, Tsuguhide

doi:10.1021/ac00205a022

Cited by 148 publications

(47 citation statements)

References 8 publications

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“…A different approach to the applications of polymers in CE has been introduced by Terabe and Isemura [18], i.e., ion-exchange electrochromatography using charged polymers to enhance separation selectivity of organic isomer ions. Following this idea, Stathakis and Cassidy proposed cationic polymers for selectivity control in the CE separations of inorganic anions [19].…”

Section: Introductionmentioning

confidence: 99%

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Cifuentes

Poppe

Kraak

et al. 1996

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Section: Introductionmentioning

confidence: 99%

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Cifuentes

Poppe

Kraak

et al. 1996

Journal of Chromatography B: Biomedical Sciences and Applicatio

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“…3 Many researchers focus their work on the development of novel methods concerning MEKC by using modifiers. For example, cyclodextrin-modified MEKC (CD-MEKC) [4][5][6][7] borate-complex EKC 8,9 and ion-exchange EKC 10,11 have been reported. Each of these methods has extended the application of MEKC to the analysis of various types of compounds.…”

mentioning

confidence: 99%

Reversal Behaviors of the Enantiomer Migration Order and the Stereo-selectivity of Cu(II) Complex with Amino Acid Enantiomers in Ligand Exchange-Micellar Electrokinetic Chromatography

et al. 2000

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Since the pioneering work of Terabe et al. 1,2 micellar electrokinetic chromatography (MEKC) has become a powerful technique for the analysis of both neutral and charged samples. 3 Many researchers focus their work on the development of novel methods concerning MEKC by using modifiers. For example, cyclodextrin-modified MEKC (CD-MEKC) 4-7 borate-complex EKC 8,9 and ion-exchange EKC 10,11 have been reported. Each of these methods has extended the application of MEKC to the analysis of various types of compounds. The mechanism of the ligand exchange was reported for the separation of dansyl amino acid enantiomers by liquid chromatography 12,13 and subsequently capillary electrophoresis. 13,14 Ligand exchange-micellar electrokinetic chromatography (LE-MEKC), a hybrid technique of the ligand exchange mechanism and MEKC, has been receiving increasing attention, because it offers the possibility of the simultaneous separation of optical isomers with the differences in the positional structure, such as o-, m-, p-enantiomers of amino acids, which contain a phenyl ring with different substituents 16 as well as positional and optical isomers of the tryptophan family. 17 Based on the ligand exchange mechanism, it is the difference in the stability constants that give the chiral separation of enantiomers. In other words, the EMO depends on the stability of the complex. Therefore, EMO can be predicted based on the stability of the complex. Conversely, the EMO can be applied in a study of complex stability.In order to explore the mechanism of LE-MEKC and to provide some scientific evidence for predicting and controlling the EMO or for studying the complex stability of a ternary Cu(II) complex with different amino acid enantiomers, it is significant to study the separation behavior of enantiomers in LE-MEKC. In fact, many works have thrown light on the reversal of the EMO. 14,15,18-22 Our previous work involved the separation behavior of amino acid enantiomers in LE-MEKC, 23 in which we discussed the reversal of EMO of amino acids and o-, m-and p-order of enantiomers with the introduction of micellar phases. In this paper we focus our study on the influence of ligand chirality, trans-and cis-conformation of 4-hydroxy group in hydroxyproline and the SDS micellar phase on the separation behavior when using different ligands of the proline family in LE-MEKC. Experimental InstrumentationThe CE instrumental setup involves a HEL5-30P2-TTu high voltage power supply (Matsusada Precision Devices Inc., Japan), a CE-971 UV detector (Jasco Corporation, Japan) and a C-R6A Chromatopac Recorder (Shimadzu, Japan). Separations were carried out in fused-silica capillaries (0.050 mm i.d.×0.375 mm o.d.) from GL Sciences Inc. (Japan) with a total length of 56 cm and an effective length of 40 cm. ChemicalsAll of the chemicals were of reagent grade and were used as Hachioji, Japan The dependence of the enantioselectivity and enantiomer migration order (EMO) on the chirality and stereo-conformation of ligands used for the chiral selectors of Cu(...

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“…The use of polyelectrolytes in CE was established in the early 90's for EOF control and as a pseudophase in ionexchange electrokinetic chromatography [35][36][37][38]. While this approach has been used extensively for EOF control (readers are directed to a recent review on polymeric coatings for more details [20]), the buildup of multiple layers of oppositely charged polymers has only recently been applied to CEC where it was shown to provide a very stable and reproducible coating [26][27][28].…”

Section: Resultsmentioning

confidence: 99%

Towards a microchip‐based chromatographic platform. Part 2: Sol‐gel phases modified with polyelectrolyte multilayers for capillary electrochromatography

Breadmore

Shrinivasan

Karlinsey³

et al. 2003

Electrophoresis

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The potential for using polyelectrolyte multilayers (PEMs) to provide chromatographic functionality on continuous silica networks created from sol-gel chemistry has been evaluated by capillary electrochromatography (CEC). Construction of the PEM was achieved by flushing the column with polyelectrolytes of alternative charge, with variation of the properties of the exposed polyelectrolyte providing a unique means to vary the chromatographic surface. Variation of the exposed polyelectrolyte from poly(diallyldimethylammonium chloride) (PDDAC) to dextran sulfate (DS) allowed the direction of the electroosmotic flow (EOF) to be changed and also provided a means to vary the chromatographic capacity. Variation of negative polymer from DS to poly(styrene sulfonate) (PSS) significantly altered the EOF and the migration of peptides, with both the reversed-phase and ion-exchange capacities increasing. An alternative method for changing the column capacity was to change the thickness of the PEM, which was evaluated by anion-exchange CEC. A 70-80% increase in retention was observed for all anions without any increase in EOF suggesting significant penetration of the analytes through the PEM and interaction with buried charges within the PEM.

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Ion-exchange electrokinetic chromatography with polymer ions for the separation of isomeric ions having identical electrophoretic mobilities

Cited by 148 publications

References 8 publications

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Reversal Behaviors of the Enantiomer Migration Order and the Stereo-selectivity of Cu(II) Complex with Amino Acid Enantiomers in Ligand Exchange-Micellar Electrokinetic Chromatography

Towards a microchip‐based chromatographic platform. Part 2: Sol‐gel phases modified with polyelectrolyte multilayers for capillary electrochromatography

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