2010
DOI: 10.1002/dvdy.22323
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Ion imaging during axolotl tail regeneration in vivo

Abstract: Several studies have reported that endogenous ion currents are involved in a wide range of biological processes from single cell and tissue behavior to regeneration. Various methods are used to assess intracellular and local ion dynamics in biological systems, e.g., patch clamping and vibrating probes. Here, we introduce an approach to detect ion kinetics in vivo using a noninvasive method that can electrophysiologically characterize an entire experimental tissue region or organism. Ion-specific vital dyes hav… Show more

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Cited by 30 publications
(33 citation statements)
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“…Injury is known to affect cellular processes including cytoskeletal dynamics, cell–cell contacts and membrane potential. Previous work in planaria, axolotl and Xenopus have demonstrated dynamic changes in the resting membrane potential (Vmem) occur after injury and this response is essential for faithful regeneration to occur (Beane et al, 2013; Blackiston et al, 2009; Borgens, 1986; Ozkucur et al, 2010). However it remains unknown if a similar response occurs after SCI.…”
Section: Resultsmentioning
confidence: 99%
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“…Injury is known to affect cellular processes including cytoskeletal dynamics, cell–cell contacts and membrane potential. Previous work in planaria, axolotl and Xenopus have demonstrated dynamic changes in the resting membrane potential (Vmem) occur after injury and this response is essential for faithful regeneration to occur (Beane et al, 2013; Blackiston et al, 2009; Borgens, 1986; Ozkucur et al, 2010). However it remains unknown if a similar response occurs after SCI.…”
Section: Resultsmentioning
confidence: 99%
“…After tail amputation in Xenopus tadpoles, the activation of the H + V-ATPase is necessary and sufficient to promote tail regeneration (Adams et al, 2007). Recent work in the axolotl established that changes in calcium, sodium and membrane potential occur in cells at the injury site after tail amputation (Ozkucur et al, 2010). To determine if these early injury-induced signals were essential for spinal cord regeneration in the axolotl we injected the fluorescent voltage sensitive dye DiBAC into the spinal cord central canal and used in vivo fluorescent imaging to investigate the electrical response of the ependymoglial cells to injury.…”
Section: Discussionmentioning
confidence: 99%
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“…EFs of physiological strength (~1 V/cm), both endogenous and externally applied, are known to affect intracellular ion dynamics and induce dramatic changes in cell behavior, including cell directional motility [15]. Congruent with the overriding role of electric fields in wound healing [6], ion transport proteins have also been shown to modulate directional cell migration [711].…”
Section: Introductionmentioning
confidence: 99%
“…The cell-surface V-ATPase is also up regulated at the mRNA and protein levels within 6h of amputation -an extremely early step in the regeneration process. More recently, Özkucur et al could show that ion contents in the axolotl tail blastema change dynamically during regeneration and, in most cases, are still fluctuating at 48 h post amputationem whereas after 24 hours, downstream pathways (BMP, Notch, Msx, Wnt and Fgfs) are activated [37,38]. After 7 days this regeneration is completed.…”
Section: Equivalent Counterpart In the Organism?mentioning
confidence: 99%