Ion pair-based mobile phase additives to improve the separation of alkaloids in supercritical fluid chromatography

Yue, Zi-Xuan; Gu, Yu-Xin; Yan, Tian‐Ci; Li, Min-Hui; Zheng, Hui; Cao, Jun

doi:10.1016/j.jpba.2021.114467

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…), acting as ion-pair agents, favors their electrostatic and hydrophobic interactions with the stationary phase of the pure porous silica and PFP columns, respectively. 152,153 The use of scCO 2 without co-solvents has also been reported with the ethylene-bridged hybrid (BEH) and BEH 2-EP columns, which showed the best retention and resolution, among other columns. 154,155 The 2-EP stationary phase has been specifically designed to improve the peak shape of strongly basic compounds 156 as a good alternative for alkaloid analysis (Table 5).…”

Section: Supercritical Fluid Chromatography (Sfc) For Alkaloid Analysismentioning

confidence: 99%

“…In addition, they isolated these compounds by SFC obtaining recoveries close to 100%. Recently, Yue et al 153 developed SFC based on the ion pair reagent method. Optimized chromatographic parameters (stationary phase, modifier type and concentration, outlet pressure, temperature, and flow rate) allowed the identification of seven alkaloids from Rhyzoma corydalis in 20 min with a low LOQ (range of ppb) using a UV detector (280 nm).…”

Section: Alkaloids Analyzed By Sfcmentioning

confidence: 99%

“…), acting as ion-pair agents, favors their electrostatic and hydrophobic interactions with the stationary phase of the pure porous silica and PFP columns, respectively. 152,153…”

Section: Supercritical Fluid Chromatography (Sfc) For Alkaloid Analysismentioning

confidence: 99%

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Section: Supercritical Fluid Chromatography (Sfc) For Alkaloid Analysismentioning

confidence: 99%

Section: Alkaloids Analyzed By Sfcmentioning

confidence: 99%

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The Development of One New Normal Phase Liquid Chromatography Method and Thermodynamic Investigation of Olodaterol Hydrochloride Enantiomer

Rao,

Zhang,

Luan

et al. 2024

Chirality

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In order to improve and replace the enantiomer method outlined in the olodaterol hydrochloride draft monograph (From the European Pharmacopoeia forum), one new, simple, and fast enantioselective normal phase high‐performance liquid chromatography chiral method was developed on polysaccharide‐based Chiral MX (2) (4.6 × 250 mm, 5 μm) column. n‐Hexane, ethanol, and diethylamine in the ratio of 40:60:0.1 (V/V/V) were selected as mobile phase at a flow rate of 0.8 mL/min, and the detection was performed on a photodiode array detector at 225 nm with 5 μL injection volume. The column temperature was set at 40°C for better peak shape and sensitivity. The analysis time can be shortened to 15 min, whereas the resolution between enantiomer and olodaterol was found to be even more than 10.0, which was far better than that obtained with the reported method in this draft monograph. The developed chiral method was validated in accordance with ICH Q2 (R1), including specificity, LOD&LOQ, precision, linearity, accuracy, and robustness. Thereby, the proposed method was demonstrated to be suitable for the determination of enantiomer in olodaterol hydrochloride bulk drug and drug product. Besides, the thermodynamic parameters were evaluated on the basis of Van't Hoff plots that was used to explain correlative chiral recognition mechanisms with the chiral stationary phase.

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Additives in chiral packed column super/subcritical fluid chromatography: A little goes a long way

Roy

Tarafder

Miller

2022

Journal of Chromatography A

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Ion pair-based mobile phase additives to improve the separation of alkaloids in supercritical fluid chromatography

Cited by 5 publications

References 29 publications

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

The Development of One New Normal Phase Liquid Chromatography Method and Thermodynamic Investigation of Olodaterol Hydrochloride Enantiomer

Additives in chiral packed column super/subcritical fluid chromatography: A little goes a long way

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