Ion-pair high-performance liquid chromatographic determination of morphine and pseudomorphine in injections

Dolez̆alová, Milada

doi:10.1016/0731-7085(92)80072-u

Cited by 12 publications

(4 citation statements)

References 11 publications

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“…Two HPLC methods, one to separate morphine N-oxide and morphine and the other to separate codeine, pseudomorphine and apomorphine were developed. An HPLC method for assaying morphine and pseudomorphine has been reported, but morphine N-oxide was not resolved using this method (17).…”

Section: Hplcmentioning

confidence: 99%

Study protocol: stability of morphine injected without preservative, delivered with a disposable infusion device

Oustric-Mendes

Huart

Hoang³

et al. 1997

Journal of Clinical Pharmacy and Therapeutics

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Section: Hplcmentioning

confidence: 99%

Study protocol: stability of morphine injected without preservative, delivered with a disposable infusion device

Oustric-Mendes

Huart

Hoang³

et al. 1997

Journal of Clinical Pharmacy and Therapeutics

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“…Various HPLC methods are available for the assay of morphine, e.g. ion-pair reversed-phase chroma tography, ion-exchange chromatography or separation by special solid phase derivatives and analysis after derivatization (5,(9)(10)(11)(12)(13). The procedure used in this study was based on an ion-pair method described in the USP XXII (14).…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…The results demonstrate that even in the absence of stabilizers, morphine chloride solutions may safely be sterilized for 15 min at 121°C. The HPLC method was shown to be sufficiently sensi- oxygen and temperature (5)(6)(7)(8). The main decomposition products of morphine are pseudomorphine and morphine N-oxide formed in a relative ratio of 9 : I, but other degradation products are also formed (6, 8).…”

mentioning

confidence: 99%

Stability of high-dose morphine chloride injection upon heat sterilization: comparison of UV-spectroscopy and HPLC

Steger

Martinelli

Mühlebach

1996

Journal of Clinical Pharmacy and Therapeutics

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Aarau, CH-5001 h r a u , Switzerland SUMMARYThe stability of a preservative-free morphine chloride solution for intravenous or intrathecal use manufactured at a concentration of 40 mglml, near the solubility limit in water, was studied. The influence of heat and oxygen on morphine content was measured with and without autoclaving, and after additional thermal and oxidative stress. The morphine injection was stable during steam sterilization at 121°C for up to I80 min if the solution was adjusted to a pH of 3. High-performance liquid chromatography with W detection (HPLC) and direct W spectroscopy (W) (the latter available in most hospital pharmacies for analytical purposes) were compared for specificity, precision and appropriateness for content and stability assessment of morphine solutions. UV could only be used for quantification of undecomposed morphine. Morphine degradation products of stressed solutions interfered with the direct W assay of morphine at 286 nm, whereas these interfering components were separated by the ion-pair reversed-phase HPLC used. The results demonstrate that even in the absence of stabilizers, morphine chloride solutions may safely be sterilized for 15 min at 121°C. The HPLC method was shown to be sufficiently sensi-

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“…The development of a rapid and sensitive assay should facilitate the purification and characterization of the 2,2Ј-bimorphine-producing enzyme. 2,2Ј-Bimorphine has been shown to be a spontaneous reaction product of morphine in aqueous solutions, though the reaction was extremely slow (4). Furthermore, morphine can be oxidized to 2,2Ј-bimorphine with alkaline ferricyanide, a reaction which is known to proceed via a mesomeric aryloxy free radical, leading to the formation of the dimer (1).…”

mentioning

confidence: 99%

Oxidation of Morphine to 2,2′-Bimorphine by Cylindrocarpon didymum

Stabler

Bruce

1998

Appl Environ Microbiol

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The oxidation of morphine by whole-cell suspensions and cell extracts of Cylindrocarpon didymum gave rise to the formation of 2,2′-bimorphine. The identity of 2,2′-bimorphine was confirmed by mass spectrometry and 1H nuclear magnetic resonance spectroscopy. C. didymum also displayed activity with the morphine analogs hydromorphone, 6-acetylmorphine, and dihydromorphine, but not codeine or diamorphine, suggesting that a phenolic group at C-3 is essential for activity.

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Ion-pair high-performance liquid chromatographic determination of morphine and pseudomorphine in injections

Cited by 12 publications

References 11 publications

Study protocol: stability of morphine injected without preservative, delivered with a disposable infusion device

Study protocol: stability of morphine injected without preservative, delivered with a disposable infusion device

Stability of high-dose morphine chloride injection upon heat sterilization: comparison of UV-spectroscopy and HPLC

Oxidation of Morphine to 2,2′-Bimorphine by Cylindrocarpon didymum

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