Ion release behavior of vanadium-doped mesoporous bioactive glass particles and the effect of the released ions on osteogenic differentiation of BMSCs <i>via</i> the FAK/MAPK signaling pathway

Li, Jiangfeng; Li, Junying; Wei, Yuhao; Xu, Na; Li, Jingtao; Pu, Ximing; Wang, Juan; Huang, Zhongbing; Liao, Xiaoming; Yin, Guangfu

doi:10.1039/d1tb01479j

Cited by 18 publications

(13 citation statements)

References 79 publications

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“…Our previous study demonstrated that Si ions released from MBG synergistically promoted the osteogenic differentiation of rBMSCs with Ca, P ions via the activation of Itga 2b-pFAK-MAPK (pERK1/2 and pP38) signaling pathway [ 52 ]. The dissolution of MBG in the PGC/M scaffold releases free Si, Ca and P ions and may stimulates cell response in a similar way [ [53] , [54] , [55] ].…”

Section: Discussionmentioning

confidence: 99%

MBG/ PGA-PCL composite scaffolds provide highly tunable degradation and osteogenic features

Wang

Gao

et al. 2022

Bioactive Materials

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Section: Discussionmentioning

confidence: 99%

MBG/ PGA-PCL composite scaffolds provide highly tunable degradation and osteogenic features

Wang

Gao

et al. 2022

Bioactive Materials

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“…However, when FAK gene expression is inhibited by the FAK inhibitor (PF-573228), the expression of ERK and p38 genes is obviously inhibited in contrast to the corresponding group without the FAK inhibitor, but the BMP-2 and COL-1 genes are significantly up-regulated. 26 These results suggest that V(V) ions may also promote the expression of osteogenic genes through other signaling pathways.…”

Section: Introductionmentioning

confidence: 91%

“…23 Our previous studies have demonstrated that the incorporation of V(V) into MBGs can enhance the degradation of MBG to trigger the continuous release of V(V), 24,25 and the released V(V) ions promote osteogenesis through the MAPK signaling pathway. 26 The study reveals that expression of FAK and ITGA2b genes is up-regulated by four-fold dilution of 1V-MBG extract (denoted V1/4) in contrast to the same dilution ratio of MBG extract (written as M1/4). For MAPK signaling pathway downstream ERK, p38, and JNK genes, the V1/4 group significantly increases the expression of ERK1 and p38 genes, while the JNK gene is insignificantly different compared with the control group.…”

Section: Introductionmentioning

confidence: 95%

“…Vanadium (V) and its compounds are known as insulin mimics or insulin enhancers, and recent animal experiments have found that V can also be incorporated into the lattice of bone minerals to replace phosphate without lattice distortion and may even increase the toughness of the bone . Our previous studies have demonstrated that the incorporation of V(V) into MBGs can enhance the degradation of MBG to trigger the continuous release of V(V), , and the released V(V) ions promote osteogenesis through the MAPK signaling pathway . The study reveals that expression of FAK and ITGA2b genes is up-regulated by four-fold dilution of 1V-MBG extract (denoted V1/4) in contrast to the same dilution ratio of MBG extract (written as M1/4).…”

Section: Introductionmentioning

confidence: 96%

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Vanadium-Doped Mesoporous Bioactive Glass Promotes Osteogenic Differentiation of rBMSCs via the WNT/β-Catenin Signaling Pathway

Cong,

Zhang,

et al. 2023

ACS Appl. Bio Mater.

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Pentavalent vanadium [V(V)] has been studied as bioactive ions to improve the bone defect repair; however, its osteogenic promotion mechanism is still not fully understood so far. In this study, a V-doped mesoporous bioactive glass (V-MBG) was prepared, and its effects on osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) and potential signaling pathways were investigated. The physicochemical characterization revealed that the incorporation of V slightly reduced the specific surface area and increased the mesoporous pore size, and the abundant mesopores of V-MBG were beneficial to the sustained dissolution of V(V) ions as well as calcium, silicon, and phosphorus ions. Cell proliferation results indicated that the high dilution ratio (>16) V-MBG extract markedly promoted the proliferation of rBMSCs compared with the control group and the same dilution ratio MBG extract. Compared with the same dilution ratio MBG extract, diluted V-MBG extracts markedly promoted the secretion of alkaline phosphatase (ALP) and osteocalcin (OCN) protein at day 7 but insignificantly stimulated the runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF) protein synthesis. In depth, the diluted V-MBG extracts remarkably up-regulated the expression of WNT/β-catenin pathway direct target genes, including WNT3a, β-catenin, and AXIN2 genes in contrast to the same dilution ratio MBG extracts, suggesting that the released V(V) ions might promote osteogenic differentiation of rBMSCs via the WNT/β-catenin signaling pathway.

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“…Titanium alloy, which has been widely used as bone repair material, has obvious demerits. On the one hand, titanium alloy is bioinert and has a low binding strength with bone tissues [3,4]. On the other hand, the mechanical strength of titanium alloy is much higher than that of natural bone tissue, making titanium alloy prone to stress shielding after implantation.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Graphene on the Biocompatibility of a 3D-Printed Porous Titanium Alloy

et al. 2021

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3D-printed titanium (Ti) materials have attracted much attention in the field of bone tissue repair. However, the combination strength of traditional alloy materials with bone tissue is lower, and the elastic modulus is higher than that of natural bone tissue, which makes the titanium alloy susceptible to stress shielding phenomena after implantation. Therefore, it is urgent to find better surface modification technology. In this study, the physical and chemical properties, toxicity, and proliferation of adipose stem cells of composite graphene-coated titanium alloy (Gr–Ti) were investigated using 3D-printed titanium alloy as a material model. Physical and chemical property tests confirmed that 3D printing could produce porous titanium alloy materials; the compressive strength and elastic modulus of the titanium alloy scaffolds were 91 ± 3 MPa and 3.1 ± 0.4 GPa, matching the elastic modulus of normal bone tissue. The surface characterization shows that graphene can be coated on titanium alloy by a micro-arc oxidation process, which significantly improves the surface roughness of titanium alloy. The roughness factor (Ra) of the Ti stent was 4.95 ± 1.12 μm, while the Ra of the Gr–Ti stent was 6.37 ± 0.72 μm. After the adipose stem cells were co-cultured with the scaffold for 4 h and 24 h, it was found that the Gr–Ti scaffold could better promote the early cell adhesion. CCK-8 tests showed that the number of ADSCs on the G–Ti scaffold was significantly higher than that on the Ti scaffold (p < 0.01). The relative growth rate (RGR) of ADSCs in Gr–Ti was grade 0–1 (non-toxic). In the in vivo experiment of repairing a critical bone defect of a rabbit mandible, the bone volume fraction in the Gr–Ti group increased to 49.42 ± 3.28%, which was much higher than that in the Ti group (39.76 ± 3.62%) (p < 0.05). In conclusion, the porous graphene–titanium alloy promotes the proliferation and adhesion of adipose stem cells with multidirectional differentiation potential, which has great potential for the application of bone tissue engineering in repairing bone defects in the future.

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Ion release behavior of vanadium-doped mesoporous bioactive glass particles and the effect of the released ions on osteogenic differentiation of BMSCs via the FAK/MAPK signaling pathway

Abstract: Vanadium is an important trace element in bone to involve in bone metabolism, bone formation, and bone growth, but roles of various vanadium ions, especially pentavalent vanadium, in bone tissue...

Cited by 18 publications

References 79 publications

MBG/ PGA-PCL composite scaffolds provide highly tunable degradation and osteogenic features

MBG/ PGA-PCL composite scaffolds provide highly tunable degradation and osteogenic features

Vanadium-Doped Mesoporous Bioactive Glass Promotes Osteogenic Differentiation of rBMSCs via the WNT/β-Catenin Signaling Pathway

The Effects of Graphene on the Biocompatibility of a 3D-Printed Porous Titanium Alloy

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