Ion Trapping, Storage, and Ejection in Structures for Lossless Ion Manipulations

Zhang, Xinyu; Garimella, Sandilya; Prost, Spencer; Webb, Ian; Chen, Tsung Chi; Tolmachev, Aleksey V.; Norheim, Randolph V.; Baker, Erin; Anderson, Gordon A.; Ibrahim, Yehia; Smith, Richard

doi:10.1021/acs.analchem.5b00214

Cited by 52 publications

(78 citation statements)

References 47 publications

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“…A drift time reference approach, which corrects slight differences in peak apex positions by alternating between the sample and an internal standard, would be very useful for obtaining accurate drift times from traditional IMS measurements and correcting run-to-run and inter-lab variability. We also expect that in the near future even faster and more sophisticated isomer separations will be possible from recent developments in structures for lossless ion manipulation (SLIM) technology [43]. SLIM technology enables the construction of serpentine extended path IMS drift regions, significantly boosting current capabilities by allowing lipid isomers with limited separation in current platforms to be baseline separated while also reducing the need for long LC gradient times.…”

Section: Discussionmentioning

confidence: 99%

Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry

Kyle

Zhang

Weitz

et al. 2016

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Understanding how biological molecules are generated, metabolized and eliminated in living systems is important for interpreting processes such as immune response and disease pathology. While genomic and proteomic studies have provided vast amounts of information over the last several decades, interest in lipidomics has also grown due to improved analytical technologies revealing altered lipid metabolism in type 2 diabetes, cancer, and lipid storage disease. Mass spectrometry (MS) measurements are currently the dominant approach for characterizing the lipidome by providing detailed information on the spatial and temporal composition of lipids. However, interpreting lipids’ biological roles is challenging due to the existence of numerous structural and stereoisomers (i.e. distinct acyl chain and double-bond positions), which are often unresolvable using present approaches. Here we show that combining liquid chromatography (LC) and structurally-based ion mobility spectrometry (IMS) measurement with MS analyses distinguishes lipid isomers and allows insight into biological and disease processes.

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Section: Discussionmentioning

confidence: 99%

Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry

Kyle

Zhang

Weitz

et al. 2016

Analyst

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219

203

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“…34,35 A SLIM drift IMS module was implemented using a static dc voltage gradient coapplied with the rf to the rung electrodes and provided good performance, along with ancillary capabilities such as the ability to select, trap, and accumulate selected species after separation. 35,36 Other ion manipulations demonstrated in SLIM include lossless ion transmission over a wide m / z range, 32,34 efficient trapping for as long as 5 h, accumulation with near 100% efficiency, 35 execution of 90° turns, and switching to alternative paths. 31,33 Although drift IMS has been demonstrated using SLIM, the high voltage constraints limit the drift path lengths that are practical and thus the achievable IMS resolution.…”

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confidence: 99%

Characterization of Traveling Wave Ion Mobility Separations in Structures for Lossless Ion Manipulations

et al. 2015

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We report on the development and characterization of a traveling wave (TW)-based Structures for Lossless Ion Manipulations (TW-SLIM) module for ion mobility separations (IMS). The TW-SLIM module uses parallel arrays of rf electrodes on two closely spaced surfaces for ion confinement, where the rf electrodes are separated by arrays of short electrodes, and using these TWs can be created to drive ion motion. In this initial work, TWs are created by the dynamic application of dc potentials. The capabilities of the TW-SLIM module for efficient ion confinement, lossless ion transport, and ion mobility separations at different rf and TW parameters are reported. The TW-SLIM module is shown to transmit a wide mass range of ions (m/z 200–2500) utilizing a confining rf waveform (~1 MHz and ~300 Vp-p) and low TW amplitudes (<20 V). Additionally, the short TW-SLIM module achieved resolutions comparable to existing commercially available low pressure IMS platforms and an ion mobility peak capacity of ~32 for TW speeds of <210 m/s. TW-SLIM performance was characterized over a wide range of rf and TW parameters and demonstrated robust performance. The combined attributes of the flexible design and low voltage requirements for the TW-SLIM module provide a basis for devices capable of much higher resolution and more complex ion manipulations.

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“…In this work, we introduce a new structures for lossless ion manipulations (SLIM) CID module for CID/MS. SLIM devices have been previously demonstrated for ion mobility (IM) separations[25–28], mobility-based ion selection[29], and ion trapping[30]. In this study, we demonstrate that SLIM is adaptable (and highly suitable) to applications outside of IM.…”

Section: Introductionmentioning

confidence: 67%

A Structures for Lossless Ion Manipulations (SLIM) Module for Collision Induced Dissociation

Webb

Garimella

Norheim

et al. 2016

J. Am. Soc. Mass Spectrom.

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A collision induced dissociation (CID) structures for lossless ion manipulations (SLIM) module is introduced and coupled to a quadrupole time-of-flight (QTOF) mass spectrometer (MS). The SLIM CID module was mounted after an ion mobility (IM) drift tube to enable IM/CID/MS studies. The efficiency of CID was studied by using the model peptide leucine enkephalin. CID efficiencies (62%) compared favorably to other beam-type CID methods. Additionally, the SLIM CID module was used to fragment a mixture of 9 peptides after IM separation. This work also represents the first application of SLIM in the 0.3 to 0.5 Torr pressure regime, an order of magnitude lower in pressure than previously studied.

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Ion Trapping, Storage, and Ejection in Structures for Lossless Ion Manipulations

Cited by 52 publications

References 47 publications

Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry

Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry

Characterization of Traveling Wave Ion Mobility Separations in Structures for Lossless Ion Manipulations

A Structures for Lossless Ion Manipulations (SLIM) Module for Collision Induced Dissociation

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