1984
DOI: 10.2170/jjphysiol.34.973
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Ionic basis of resting membrane potential in frog taste cells.

Abstract: Intracellular recording of the resting membrane potential was made from taste cells of the bullfrog by replacing the interstitial fluid surrounding the cells by various physiological saline solutions. The resting potential of the taste cell was -28±4 mV (mean±S.E.) after replacement of the interstitial fluid by normal Ringer solution. The resting potential was very much dependent on the interstitial K+ concentration ([K+]0). Tenfold increase in [K+]0 decreased the resting potential by 18 mV. Total removal of i… Show more

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Cited by 13 publications
(6 citation statements)
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“…Other investigators have studied the biophysical properties of the apical and basolateral membranes of taste cells in situ, particularly in frogs (Kashiwayanagi, Miyake & Kurihara, 1983;Sato et al, 1984;Okada, Miyamoto & Sato, 1986;Herness, 1987). To study properties of the basolateral membrane, the contents of the interstitial fluid surrounding the taste cell were changed either by perfusing the lingual artery or by prolonged perfusion of the surface of the tongue, relying on diffusion across the epithelium to replace the interstitial mileau.…”
Section: Discussionmentioning
confidence: 99%
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“…Other investigators have studied the biophysical properties of the apical and basolateral membranes of taste cells in situ, particularly in frogs (Kashiwayanagi, Miyake & Kurihara, 1983;Sato et al, 1984;Okada, Miyamoto & Sato, 1986;Herness, 1987). To study properties of the basolateral membrane, the contents of the interstitial fluid surrounding the taste cell were changed either by perfusing the lingual artery or by prolonged perfusion of the surface of the tongue, relying on diffusion across the epithelium to replace the interstitial mileau.…”
Section: Discussionmentioning
confidence: 99%
“…Kashiwayanagi et al (1983) described the presence of voltage-gated Na + and Ca > channels in frog taste cells but did not specifically address the question of their distribution. Sato et al (1984) and Okada et al (1986) studied passive properties of frog taste cells and reported that apical and basolateral membranes have passive K + conductive pathways. Okada et al (1986) further probed this K + conductance and found that TEA affects neither membrane.…”
Section: Discussionmentioning
confidence: 99%
“…The lack of information concerning taste receptor cells is due in part to the difficulty in recording from the small taste cells of most species. Although intracellular recordings have been made from taste receptor cells in catfish (Teeter & Kare, 1974), frogs (Sato & Beidler, 1975;Akaike, Noma & Sato, 1976;Kashiwayanagi, Miyake & Kurihara, 1983;Sato, Sugimoto, Okada & Miyamoto, 1984), mudpuppies (West & Bernard, 1978), rats (Ozeki, 1971;Ozeki & Sato, 1972) and mice (Tonosaki & Funakoshi, 1984), what has been reported to date is that taste receptor cells generally have low resting potentials (less than -40 mV), low input resistances (17-80 MQ), linear currentvoltage (I-V) relationships and strictly passive membrane properties (however, cf. Roper, 1983;Kashiwayanagi et al 1983).…”
mentioning
confidence: 99%
“…Existence of tight junctions between the supporting cells and the apical processes of the taste cells, and between the taste cell apical processes in the frog taste organ has also been reported by a number of investigators (for example see GRAzIADEI and DEHAN, 1971;NoMURA et al, 1975). Recently SATO et al (1984) divided the taste cell membrane into the receptor and basolateral membranes and showed that the properties of these two membranes are not identical. Na + entry across the apical membrane of epithelial cells is reduced by amiloride and it has been accepted that an amiloride-sensitive Na+ channel exists in the apical cell membrane (BENOS, 1982;LINDEMANN, 1984).…”
mentioning
confidence: 99%
“…Our previous experiments with isolated tongue preparations showed that both diffusion and metabolism-dependent potentials exist across the resting basolateral membrane of a frog taste cell and these play an important role in generation of the resting potential (SATO et al, 1984). It still remains unsolved what kinds of ion transport systems exist in the taste receptor membrane, and how the receptor membrane contributes to the generation of the resting potential of the taste cell.…”
mentioning
confidence: 99%