Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse

Jagger, Daniel J.; Holley, Matthew C.; Ashmore, Jonathan

doi:10.1007/s004240050873

Cited by 13 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following application of acetylcholine the inward currents were clearly visible because the outward, calcium-activated potassium conductance was absent (see Evans, 1996), consistent with the fact that it is normally expressed a few days later (Dulon and Lenoir, 1996). There is evidence for expression of the outward current after longer periods in culture (Jagger et al, 1999). This suggests that the sequence of gene expression in UB/OC-1 is similar to that observed in vitro although the time scale is longer.…”

Section: Cochlear Cell Lines At E135 (Ub/oc-1; Ub/oc-2)supporting

confidence: 54%

Cell lines in inner ear research

Rivolta

Holley

2002

J. Neurobiol.

View full text Add to dashboard Cite

Section: Cochlear Cell Lines At E135 (Ub/oc-1; Ub/oc-2)supporting

confidence: 54%

Cell lines in inner ear research

Rivolta

Holley

2002

J. Neurobiol.

View full text Add to dashboard Cite

“…The currents recorded under proliferating culture conditions and the I K,early current are similar to those recorded from other cochlear epithelial cell lines (Jagger et al, 1999;Lawlor et al, 1999). The reversal potentials suggest that they are mainly carried by K + ions.…”

Section: Membrane Conductances In Vot-e36supporting

confidence: 52%

A model for mammalian cochlear hair cell differentiation in vitro: effects of retinoic acid on cytoskeletal proteins and potassium conductances

Helyer¹,

Cacciabue-Rivolta²,

Davies³

et al. 2007

Eur J of Neuroscience

Self Cite

View full text Add to dashboard Cite

We have established a model for the in-vitro differentiation of mouse cochlear hair cells and have used it to explore the influence of retinoic acid on proliferation, cytoskeletal proteins and voltage-gated potassium conductances. The model is based on the conditionally immortal cell line University of Sheffield/ventral otocyst-epithelial cell line clone 36 (US/VOT-E36), derived from ventral otic epithelial cells of the mouse at embryonic day 10.5 and transfected with a reporter for myosin VIIa. Retinoic acid did not increase cell proliferation but led to up-regulation of myosin VIIa and formation of prominent actin rings that gave rise to numerous large, linear actin bundles. Cells expressing myosin VIIa had larger potassium conductances and did not express the cyclin-dependent kinase inhibitor p27(kip1). US/VOT-E36 endogenously expressed the voltage-gated potassium channel alpha-subunits Kv1.3 and Kv2.1, which we subsequently identified in embryonic and neonatal hair cells in both auditory and vestibular sensory epithelia in vivo. These subunits could underlie the embryonic and neonatal delayed-rectifiers recorded in nascent hair cells in vivo. Kv2.1 was particularly prominent on the basolateral membrane of cochlear inner hair cells. Kv1.3 was distributed throughout all hair cells but tended to be localized to the cuticular plates. US/VOT-E36 recapitulates a coherent pattern of cell differentiation under the influence of retinoic acid and will provide a convenient model for screening the effects of other extrinsic factors on the differentiation of cochlear epithelial cell types in vitro.

show abstract

“…This would allow us to ascertain the time course of development of K + currents in OHCs and IHCs. The results should help us to link the expression of physiologically important hair cell genes to the age-dependent expression of specific regulatory genes and to interpret findings from emerging studies on conditionally immortal cell lines Jagger et al, 1999].…”

Section: Introductionmentioning

confidence: 99%

Development of Outward Potassium Currents in Inner and Outer Hair Cells from the Embryonic Mouse Cochlea

Helyer

Kennedy²,

Davies³

et al. 2004

Audiol Neurotol

View full text Add to dashboard Cite

We recorded K⁺ currents in inner (IHCs) and outer (OHCs) hair cells from mice at embryonic days 16 and 18 and on the day of birth (PO) to characterize their early physiological differentiation. In both cell types, outward currents increased in size during late embryonic development, in cells situated in both the apical and basal coils of the cochlea. Currents increased up to six-fold, with current density increasing four-fold. Currents in basal cells were generally larger than in the apex, and currents in IHCs were larger than in OHCs at any given stage. In OHCs, they were initially non-inactivating but gained the partial inactivation characteristic of the K⁺ current of neonatal mouse cochlear hair cells, I_K,neo, by day 18 in the base and by P0 in the apex. In IHCs, there was little change, other than in amplitude, with partial inactivation already evident in the base by embryonic day 16. These results suggest that changes in the channel complement of OHCs occur within a few days of terminal mitosis, whereas in IHCs any such development would occur earlier. The progressive development of K⁺ currents correlates with a developmental delay of around 2 days from the base to the apex of the cochlea.

show abstract

Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse

Cited by 13 publications

References 34 publications

Cell lines in inner ear research

Cell lines in inner ear research

A model for mammalian cochlear hair cell differentiation in vitro: effects of retinoic acid on cytoskeletal proteins and potassium conductances

Development of Outward Potassium Currents in Inner and Outer Hair Cells from the Embryonic Mouse Cochlea

Contact Info

Product

Resources

About