Ionic effects on microalgae harvest via microalgae-fungi co-pelletization

Zamalloa, Carlos; Gultom, Sarman Oktovianus; Rajendran, A.; Hu, Bo

doi:10.1016/j.bcab.2016.12.007

Cited by 42 publications

(18 citation statements)

References 51 publications

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“…The algal biomass contribution to the total consortium weight was of 79 ± 0.4%, which suggests an efficient method to obtain algae-rich biomass in co-culture systems. The observations demonstrate a few important points, namely that the harvesting the fungal-algal system at an early time may be preferable to avoid fungal proliferation and dominance to the consortium [25,26], and the consortium system is possibly providing a cellular stress to both strains, due to the increased lipid content when compared to the axenic cultures, following a similar effect as those reported by Reis et al [27].…”

Section: The Effect Of Different Strategies On the Lipid Accumulationsupporting

confidence: 81%

Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

et al. 2020

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This study aims to evaluate the potential of consortium biomass formation between Mucor circinelloides, an oleaginous filamentous fungal species, and Chlorella vulgaris, in order to promote a straightforward approach to harvest microalgal cells and to evaluate the lipid production in the consortium system. A synthetic medium with glucose (2 g·L−1) and mineral nutrients essential for both fungi and algae was selected. Four different inoculation strategies were assessed, considering the effect of simultaneous vs. separate development of fungal spores and algae cells, and the presence of a supporting matrix aiming at the higher recovery of algae cell rates. The results were evaluated in terms of consortium biomass composition, demonstrating that the strategy using a mature fungal mycelium with a higher algae count may provide biomass samples with up to 79% of their dry weight as algae, still promoting recovery rates greater than 97%. The findings demonstrate a synergistic effect on the lipid accumulation by the fungal strain, at around a fourfold increase when compared to the axenic control, with values in the range of 23% of dry biomass weight. Furthermore, the fatty acid profile from the samples presents a balance between saturated and unsaturated fatty acids that is likely to present an adequate balance for applications such as biodiesel production.

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Section: The Effect Of Different Strategies On the Lipid Accumulationsupporting

confidence: 81%

Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

et al. 2020

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“…According to Gultom and Hu [ 10 ], fungal spores have crystal-like layers, which prevent aggregation within themselves and with other strains; however, growing hyphae act as the main promoter of interconnections between germinating spores and other species. Microscopically, lectin carbohydrate is an important factor in the binding of algae and fungi [ 10 , 41 , 42 ]. The presence of calcium ions, which act as a link between the negatively charged cells and extracellular nucleic acids and, thus, allow cell-to-cell communication through electrostatic interactions, is particularly involved in the role of the lectin-carbohydrate binding [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

et al. 2022

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In this study, a lichen-like structure was obtained through the production of a unique biomass, formed by algae cells of Scenedesmus obliquus adhering to the mycelium of filamentous fungal Mucor circinelloides. This structure was composed in two steps; in the first one, microalgal cells and spores were incubated separately, and in the second one, after 72 h of growth, isolated, mature mycelium was harvested and added to cell culture. For spores’ incubation, a culture medium containing only 2 g·L−1 of glucose and minerals was used. This culture medium, with low sugar content, provided a fungal biomass to the anchorage of microalgae cells. WC medium was used without and with sugarcane molasses supplementation for microalgae cells’ incubation. The lichen-type structure that was formed resulted in 99.7% efficiency in the recovery of microalgae cells and in up to 80% efficiency in the recovery of algae biomass in the lichen biomass composition. In addition, the resulting consortium attained a satisfactory lipid accumulation value (38.2 wt%) with a balanced fatty acid composition of 52.7% saturated plus monounsaturated fatty acids and 47.4% polyunsaturated fatty acids. Since fungal species are easy to recover, unlike microalgae, the lichen-like structure produced indicates an efficient low-cost bioremediation and harvesting alternative; in addition, it provides an oleaginous biomass for various industrial applications.

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“…The pellets formed during the growth process can easily be separated by filtration due to their higher density compared to water. Co-pelletization process has been adopted by researchers to increase the lipid yield and biomass of microalgae [32,44]. Srinuanpan et al developed the method of the photoautotrophic cultivation of oleaginous microalgae and filamentous fungi in a co-pelletization mode.…”

Section: Cell Pelletizationmentioning

confidence: 99%

“…Most of the microalgae cells were attached to pellets within 72 h of cultivation. The effect of the ions present in the water was tested and it was observed that without Ca 2+ and Mg 2+ , the harvest efficiency was over 90% [44].…”

Section: Cell Pelletizationmentioning

confidence: 99%

Cultivation of Oily Microalgae for the Production of Third-Generation Biofuels

et al. 2019

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Biofuel production by oleaginous microalgae is a promising alternative to the conventional fossil fuels. Many microalgae species have been investigated and deemed as potential renewable sources for the production of biofuel, biogas, food supplements and other products. Oleaginous microalgae, named for their ability to produce oil, are reported to store 30–70% of lipid content due to its metabolic properties under nutrient starvation conditions. This review presents the assortment of the research studies focused on biofuel production from oleaginous microalgae. The new methods and technologies developed for oleaginous microalgae cultivation to improve their biomass content and lipid accumulation capacity were reviewed. The production of renewable, carbon neutral, bio-based or microalgae-based transport fuels are necessary for environmental protection and economic sustainability. Microalgae are a significant source of renewable biodiesel because of their ability to produce oils in the presence of sunlight more efficiently than that of crop oils. This review will provide the background to understanding the bottlenecks and the need for improvement in the cultivation or harvesting process for oleaginous microalgae.

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Ionic effects on microalgae harvest via microalgae-fungi co-pelletization

Cited by 42 publications

References 51 publications

Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

Cultivation of Oily Microalgae for the Production of Third-Generation Biofuels

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