Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

Abdelhamid, Hani Nasser

doi:10.3390/mps1020023

Cited by 21 publications

(19 citation statements)

References 162 publications

(227 reference statements)

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“…MS depends on the ionization of the viral contents, e.g., protein or RNA. Soft ionization techniques such as matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are promising for direct detection of the intact virus [25,41,76,[249][250][251][252][253][254][255][256]. Nanoparticles are essential as the surface for MALDI-MS, i.e., surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS).…”

Section: Other Methodsmentioning

confidence: 99%

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

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Section: Other Methodsmentioning

confidence: 99%

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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“…to volatile and hazardous organic solvents, ILs have been widely applied in separation science [10][11][12], analytical chemistry [8,13,14], biomass transformation [15], and organic synthesis [16], among others. Over the past years, carbohydrate chemists have witnessed the use of ILs in a wide variety of aspects [6,7,12].…”

Section: F I G U R E 1 Chemical Structures Of Ilsmentioning

confidence: 99%

“…ILs are recognized as tunable designer solvents with an enormous number of ion combinations (10 18 ) . As alternatives to volatile and hazardous organic solvents, ILs have been widely applied in separation science , analytical chemistry , biomass transformation , and organic synthesis , among others. Over the past years, carbohydrate chemists have witnessed the use of ILs in a wide variety of aspects .…”

Section: Introductionmentioning

confidence: 99%

A novel strategy to utilize ethylene glycol‐ionic liquids for the selective precipitation of polysaccharides

Zhao

et al. 2019

J of Separation Science

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In the present work, three hydrophilic ionic liquids based on the combination between imidazolium cations attached with ethylene glycol polymers of various lengths and hexafluorophosphate anion were designed and synthesized for the separation of polysaccharides. By employing dextran 100 kDa as model compound, the effects of ionic liquid content, solvent/anti‐solvent volume, and temperature on its recovery efficiency were investigated systematically. The ability of these ionic liquids to precipitate dextran 100 kDa, increases with the elongation of ethylene glycol polymer chain. The established ionic liquid‐based precipitation system was successfully applied to selectively precipitate polysaccharides from water extracts of three traditional Chinese medicines and the precipitation could be achieved in about 15 min. In addition, the different precipitation responses of acidic, neutral, and basic polysaccharides in the ionic liquid‐based precipitation system and theoretical calculations both suggested that the selective precipitation of polysaccharides was probably mediated by interaction between ionic liquids and polysaccharides. The proposed strategy facilitated the isolation and purification of polysaccharides and may trigger a novel application of ionic liquids in carbohydrate research.

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“…Unfortunately, the use of ILMs provides a stronger tendency for alkali metal–ion adduct generation 17 . In addition, the optimization of ILMs is a trial‐and‐error process, and the base‐to‐acid ratio can alter the performance of these materials 18 . Thus, sample preparation is still a bottleneck in achieving reproducible data in MALDI‐MS and MSI analyses.…”

Section: Introductionmentioning

confidence: 99%

Desorption ionization using through‐hole alumina membrane offers higher reproducibility than 2,5‐dihydroxybenzoic acid, a widely used matrix in Fourier transform ion cyclotron resonance mass spectrometry imaging analysis

Hasan

Eto

Mamun

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale DIUTHAME (desorption ionization using through‐hole alumina membrane), a recently developed matrix‐free ionization‐assisting substrate, was examined for reproducibility in terms of mass accuracy and intensity using standard lipid and mouse brain sections. The impregnation property of DIUTHAME significantly improved the reproducibility of mass accuracy and intensity compared with 2,5‐dihydroxybenzoic acid (DHB). Methods Frozen tissue sections were mounted on indium tin oxide‐coated glass slides. DIUTHAME and DHB were applied to individual sections. Subsequently, a solution of a phosphatidylcholine standard, PC(18:2/18:2), was poured onto the DIUTHAME and matrix. Finally, the samples were subjected to laser desorption ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry. The reproducibility was tested by calculating the mean ± standard deviation values of mass errors and intensities of individual ion species. Results Analysis of the PC(18:2/18:2) standard showed significantly (p < 0.01) lower mass error for DIUTHAME‐MS than for MALDI‐MS. Endogenous PC(36:4) analysis in mouse brain section also showed significantly (p < 0.05) lower mass errors for DIUTHAME‐MS. Furthermore, we investigated the mass error of some abundant lipid ions in brain sections and observed similar results. DIUTHAME‐MS displayed lower signal intensity in standard PC analysis. Interestingly, it offered higher signal intensities for all the endogenous lipid ions. Lower fluctuations of both mass accuracies and signal intensities were observed in DIUTHAME‐MS. Conclusions Our results demonstrated that DIUTHAME‐MS offers higher reproducibility for mass accuracies and intensities than MALDI‐MS in both standard lipid and mouse brain tissue analyses. It can potentially be used instead of conventional MALDI‐MS and mass spectrometry imaging analyses to achieve highly reproducible data for mass accuracy and intensity.

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Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

Cited by 21 publications

References 162 publications

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

A novel strategy to utilize ethylene glycol‐ionic liquids for the selective precipitation of polysaccharides

Desorption ionization using through‐hole alumina membrane offers higher reproducibility than 2,5‐dihydroxybenzoic acid, a widely used matrix in Fourier transform ion cyclotron resonance mass spectrometry imaging analysis

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