Ionic liquid-based aqueous two-phase extraction within a microchannel system

Novak, Uroš; Pohar, Andrej; Plazl, Igor; Žnidaršič–Plazl, Polona

doi:10.1016/j.seppur.2012.01.033

Cited by 94 publications

(63 citation statements)

References 40 publications

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“…They have been successfully employed to separate several solutes such as: sulfonamides in milk [32], cytochrome C [33], bovine serum albumin [34], antibiotics in honey [35], textile dyes [4], among others. Fig.…”

Section: Partition Behavior Of Yr In Il-salt Aqueous Two-phase Systemmentioning

confidence: 99%

Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

Alvarenga

Fideles

Silva

et al. 2015

Fluid Phase Equilibria

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Section: Partition Behavior Of Yr In Il-salt Aqueous Two-phase Systemmentioning

confidence: 99%

Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

Alvarenga

Fideles

Silva

et al. 2015

Fluid Phase Equilibria

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“…IL-based ABS have been used for the extraction of proteins and enzymes, while demonstrating to provide higher extraction efficiencies than the traditional PEG-based ABS [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Alkylimidazolium-based ILs combined with phosphate-based salts were the most widely investigated phase-forming components of ABS [15].…”

Section: Introductionmentioning

confidence: 99%

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Taha

Quental

Correia

et al. 2015

Process Biochemistry

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Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures. In this work, the extraction ability for bovine serum albumin (BSA) of aqueous biphasic systems (ABS) formed by polypropylene glycol 400 (PPG 400) and several GB-ILs was evaluated. ABS formed by PPG 400 and cholinium chloride ([Ch]Cl), GBs, and sucrose were also investigated for comparison purposes. It is shown that BSA preferentially migrates for the GB-IL-rich phase, with extraction efficiencies of 100%, achieved in a single-step. Dynamic light scattering, and circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies were employed to evaluate the effect of the investigated cholinium-based GB-ILs on the BSA stability, and compared with results obtained for the respective GBs precursors, [Ch]Cl and sucrose, a well-known protein stabilizer. Molecular docking studies were also carried out to investigate on the binding sites of GB-IL ions to BSA. The experimental results confirm that BSA has a higher stability in GB-ILs than in any of the other compounds investigated.

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“…To study the effect of K 2 HPO 4 on partitioning of the phycocyanin in ATPS, K 2 HPO 4 at different concentrations (23,25,27, 29 and 31% w/w) were mixed with 23%(w/w) [Bmim]Cl in ATPS. Based on E and Purity, the ATPS rendering the most effective partitioning was chosen for further study.…”

Section: Effect Of Concentration Of K 2 Hpo 4 On Partitioning Of Phycmentioning

confidence: 99%

“…In general, imidazolium cation of the ILs has an aromatic π system. It appears that the π-π interaction between the imidazolium cation and the aromatic residues of the proteins was a possible driving force for the extraction of proteins [25]. The probably reason was that more phycocyanin was partitioned to the top phase as the concentration increased to a higher value while residual proteins partitioned to the top phase also increased.…”

Section: Sodium Dodecyl Sulphate-gel Electrophoresismentioning

confidence: 99%

Extraction and Separation of Phycocyanin from <i>Spirulina</i> using Aqueous Two-Phase Systems of Ionic Liquid and Salt

Zhang¹,

Zhang²,

Luo³

et al. 2014

JFNR

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To explore a new and simple rapid extraction and purification technique for phycocyanin, an ionic liquids(ILs)-based aqueous two-phase system(ATPS) was developed for the purification of phycocyanin from Spirulina extracts. Effects of various process parameters such as the concentrations of [Bmim]Cl, the concentrations of KH 2 PO 4 , the concentrations of crude phycocyanin, the system pH and the temperature on partitioning of phycocyanin were evaluated. The obtained data indicated that phycocyanin was preferentially partitioned into the ILs-rich phase and the ATPS composed of 23% (w/w) [Bmim]Cland 29% (w/w) KH 2 PO 4 at 30°C and pH 7.0 showed good selectivity on phycocyanin. Under the optimum conditions, phycocyanin with a purity of 3.98 and yield of about 90.23 % was obtained. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of phycocyanin from Spirulina extracts.

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Ionic liquid-based aqueous two-phase extraction within a microchannel system

Cited by 94 publications

References 40 publications

Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Extraction and Separation of Phycocyanin from <i>Spirulina</i> using Aqueous Two-Phase Systems of Ionic Liquid and Salt

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