Ionic liquid-based transmission electron microscopy for herpes simplex virus type 1

Tsuda, Tetsuya; Kawakami, Koshi; Mochizuki, Eiko; Kuwabata, Susumu

doi:10.1007/s12551-018-0417-4

Cited by 10 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, research must be done to establish interaction trends between sample type and the RTILs so that protocols can be designed accordingly. Expansion of statistical analysis of EM‐relevant RTILs’ Kamlet‐Taft parameters [83] and their corresponding performance in EM may help answer part of this question and guide RTIL selection [40] …”

Section: Discussionmentioning

confidence: 99%

“…Recently, works produced by Tsuda et al. explored the use of RTILs in TEM imaging applications for microbial research [40] . Following a pipette‐based IL treatment protocol (similar to Figure 3), RTILs successfully facilitated the visualization of herpes simplex virus I (HSVI) in TEM [40] .…”

Section: Introductionmentioning

confidence: 99%

“…explored the use of RTILs in TEM imaging applications for microbial research [40] . Following a pipette‐based IL treatment protocol (similar to Figure 3), RTILs successfully facilitated the visualization of herpes simplex virus I (HSVI) in TEM [40] . Kamlet‐Taft parameters for polarity factors were consulted in evaluating RTILs for TEM imaging, where RTILs such as [EMI][BF 4 ] and [Ch][Lac] with a high hydrogen bond accepting ability (denoted by parameter β) performed optimally and caused no noticeable damage to specimen morphology [40] .…”

Section: Introductionmentioning

confidence: 99%

“…Following a pipette‐based IL treatment protocol (similar to Figure 3), RTILs successfully facilitated the visualization of herpes simplex virus I (HSVI) in TEM [40] . Kamlet‐Taft parameters for polarity factors were consulted in evaluating RTILs for TEM imaging, where RTILs such as [EMI][BF 4 ] and [Ch][Lac] with a high hydrogen bond accepting ability (denoted by parameter β) performed optimally and caused no noticeable damage to specimen morphology [40] . This is one of the few studies to effectively report the successful use of RTILs in TEM, while most available studies use RTIL treatment in SEM.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Electron Microscopy Imaging Applications of Room Temperature Ionic Liquids in the Biological Field: A Review

et al. 2021

View full text Add to dashboard Cite

For biological imaging using electron microscopy (EM), the use of room‐temperature ionic liquids (RTILs) has been proposed as an alternative to traditional lengthy preparation methods. With their low vapor pressures and conductivity, RTILs can be applied onto hard‐to‐image soft and/or wet samples without dehydration – allowing for a more representative, hydrated state of material and opening the possibility for visualization of in situ physiological processes using conventional EM systems. However, RTILs have yet to be utilized to their full potential by microscopists and microbiologists alike. To this end, this review aims to provide a comprehensive summary of biological applications of RTILs for EM to bridge the RTIL, in situ microscopy, and biological communities. We outline future research avenues for the use of RTILs for the EM observation of biological samples, notably i) RTIL selection and optimization, ii) applications for live cell processes and iii) electron beam and ionic liquid interaction studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electron Microscopy Imaging Applications of Room Temperature Ionic Liquids in the Biological Field: A Review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Vacuum is particularly problematic for materials with high sublimation rates. Water-containing protein crystals are a classical example of such materials, and indeed for performing TEM experiments it is generally necessary to keep them in ionic liquids (Tsuda et al, 2018) or include them in amorphous ice by cryo-plunging (Nederlof et al, 2013; Shi et al, 2013).…”

Section: Lattice Defects and Structural Modifications Induced By Temmentioning

confidence: 99%

Structure analysis of materials at the order–disorder borderline using three-dimensional electron diffraction

Mugnaioli

Gorelik

2019

Acta Crystallogr Sect B

View full text Add to dashboard Cite

Diffuse scattering, observed as intensity distribution between the Bragg peaks, is associated with deviations from the average crystal structure, generally referred to as disorder. In many cases crystal defects are seen as unwanted perturbations of the periodic structure and therefore they are often ignored. Yet, when it comes to the structure analysis of nano-volumes, what electron crystallography is designed for, the significance of defects increases. Twinning and polytypic sequences are other perturbations from ideal crystal structure that are also commonly observed in nanocrystals. Here we present an overview of defect types and review some of the most prominent studies published on the analysis of defective nanocrystalline structures by means of three-dimensional electron diffraction.

show abstract

Toward Quantitative Electrodeposition via In Situ Liquid Phase Transmission Electron Microscopy: Studying Electroplated Zinc Using Basic Image Processing and 4D STEM

Park,

Dutta,

Sun

et al. 2024

Small Methods

View full text Add to dashboard Cite

High energy density electrochemical systems such as metal batteries suffer from uncontrollable dendrite growth on cycling, which can severely compromise battery safety and longevity. This originates from the thermodynamic preference of metal nucleation on electrode surfaces, where obtaining the crucial information on metal deposits in terms of crystal orientation, plated volume, and growth rate is very challenging. In situ liquid phase transmission electron microscopy (LPTEM) is a promising technique to visualize and understand electrodeposition processes, however a detailed quantification of which presents significant difficulties. Here by performing Zn electroplating and analyzing the data via basic image processing, this work not only sheds new light on the dendrite growth mechanism but also demonstrates a workflow showcasing how dendritic deposition can be visualized with volumetric and growth rate information. These results along with additionally corroborated 4D STEM analysis take steps to access information on the crystallographic orientation of the grown Zn nucleates and toward live quantification of in situ electrodeposition processes.

show abstract

Ionic liquid-based transmission electron microscopy for herpes simplex virus type 1

Cited by 10 publications

References 11 publications

Electron Microscopy Imaging Applications of Room Temperature Ionic Liquids in the Biological Field: A Review

Electron Microscopy Imaging Applications of Room Temperature Ionic Liquids in the Biological Field: A Review

Structure analysis of materials at the order–disorder borderline using three-dimensional electron diffraction

Toward Quantitative Electrodeposition via In Situ Liquid Phase Transmission Electron Microscopy: Studying Electroplated Zinc Using Basic Image Processing and 4D STEM

Contact Info

Product

Resources

About