Ionic liquids modified Cu3(PO4)2 hybrid nanoflower for dehydrogenase immobilization by biomimetic mineralization

The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability

“…The NF-DAEs formed by Zn 2+ and 4~10 μmol/L DAE showed relatively high activities ( Figure 4 b). Our results also show the significant increase in the catalytic activities of all of the NF-DAEs, which is consistent with other reports [ 17 , 26 , 33 , 34 , 35 ]. Therefore, in the subsequent preparation of the nanoflowers, the enzyme additions in NF-DAE-Co, NF-DAE-Cu, NF-DAE-Zn, NF-DAE-Ca, NF-DAE-Ni, NF-DAE-Fe(ii), and NF-DAE-Fe(iii) were all at a level of 4 μmol/L.…”

“…In a very recent study, Xin et al reported a customized self-assembled protein–bimetallic hybrid nanoflower system, and found that most of the resulting NFs decreased the catalytic activities, except for the NF-DAE-Co/Mn [ 23 ]. As immobilized enzyme modifiers, nanoflowers could modify the enzyme’s morphological properties to produce a more biocompatible shape and release more accessible surface activities, and thus enhance the catalytic activity and stability [ 26 ]. The hybrid nanoflowers are scaled from several hundred nanometers to tens of micrometers [ 17 ], which enables the efficient interaction between substrates and enzymes.…”