2018
DOI: 10.1002/term.2744
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Ionic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes

Abstract: Oxidative stress, induced by harmful levels of reactive oxygen species, is a common occurrence that impairs proper bone defect vascular healing through the impairment of endothelial cell function. Ionic silicon released from silica-based biomaterials, can upregulate hypoxia-inducible factor-1α (HIF-1α). Yet it is unclear whether ionic Si can restore endothelial cell function under oxidative stress conditions. Therefore, we hypothesized that ionic silicon can help improve human umbilical vein endothelial cells'… Show more

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Cited by 29 publications
(22 citation statements)
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References 61 publications
(125 reference statements)
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“…Our study showed that CS reduced the ROS production in fibroblasts, indicating that CS played a role in attenuating oxidation reaction and thereby antagonized the HG‐induced cellular malfunction. This result is in accordance with previous research conducted by Monte et al who proved that Si ions have a protective role by promoting proliferation and inhibiting cellular ROS generation …”
Section: Discussionsupporting
confidence: 94%
“…Our study showed that CS reduced the ROS production in fibroblasts, indicating that CS played a role in attenuating oxidation reaction and thereby antagonized the HG‐induced cellular malfunction. This result is in accordance with previous research conducted by Monte et al who proved that Si ions have a protective role by promoting proliferation and inhibiting cellular ROS generation …”
Section: Discussionsupporting
confidence: 94%
“…Previous studies had indicated that antioxidants can improve muscle mass recovery [ 36 ], enhance viability by reducing muscle cell death [ 55 ], and promote the early stages of differentiation by inducing the expression of differentiation markers [ 28 , 56 , 57 ]. In line with this, our results indicate that Si-ions at a concentration of 0.1 mM can enhance C2C12 cell viability, proliferation, differentiation, and myogenic marker expression, such as MyoG and MyoD, analogous to the antioxidant effect of Si-ions on osteogenesis [ 41 , 47 , 48 ].…”
Section: Discussionsupporting
confidence: 74%
“…More recently, bioactive amorphous silicon oxynitride and silicon oxynitrophosphide coatings have induced rapid regeneration of vascularized bone from the antioxidant activity of sustained release silicon-ions [ 42 , 46 , 47 ]. Furthermore, amorphous silicon oxynitrophosphide coatings enhanced the angiogenic activity of endothelial cells and ionic silicon improved endothelial cell survival under toxic ROS conditions via enhanced angiogenic marker expression and antioxidant activity [ 40 , 42 , 48 ]. Additionally, research conducted by Monte et al suggests that Si ions at a concentration of 0.5 mM mitigate the toxic effects of ROS by catalyzing the conversion of superoxide to peroxide [ 21 ].…”
Section: Introductionmentioning
confidence: 99%
“…The H2O2 has been used previously for in vitro studies (29)(30) (31) as a major product of oxidative stress, mainly produced by swift conversion from superoxide (32)(33) (34) . Based on previous publications, this specific H2O2 concentration has been demonstrated to be deleterious to live tissue (19) (20) . Phosphate buffered saline (PBS) (Sigma-Aldrich, St. Louis, MO) and trypsin, buffer, and trypsin neutralizer (Lonza ®, Morristown, NJ), Angiopoietin 1 Human ELISA Kit (Invitrogen®, Waltham, MA) and HNE competitive ELISA kit (Cell Biolabs, Inc., San Diego, CA) were used for the in vitro experiments.…”
Section: In Vitro Studymentioning
confidence: 84%
“…Here, we hypothesize that a PECVD amorphous SiONx or SiONPx nanoscale coating will increase the expression of both antioxidant (SOD-1, Cat-1, Nrf2) and angiogenic (CD31, ang-1) markers, and enhance vascular tubule formation while reducing reactive oxygen species (ROS) (4-HNE, H2O2). To test these hypotheses, we investigated the effect of PECVD nanoscale coatings formed by Si, O, N and P gas reagent sources on angiogenesis under a toxic oxidative stress environment induced by 0.6 mM H2O2, which was determined previously (20) . In vitro experiments evaluated the effect on viability of HUVECs, matrix deposition, capillary tubule formation, and gene expression of angiogenic and antioxidant markers on coated and uncoated surfaces.…”
Section: Introductionmentioning
confidence: 99%