Ionic Silver and Electrical Treatment for Susceptibility and Disinfection of Escherichia coli Biofilm-Contaminated Titanium Surface

Abstract

In this work, surface disinfection and biofilm susceptibility were investigated by applying ionic silver of 0.4–1.6 µg/mL and cathodic voltage-controlled electrical treatment of 1.8 V and a current of 30 mA to Escherichia coli (E. coli) ATCC 25922 biofilm-contaminated titanium substrates. Herein, it is evident that the treatment exhibited the potential use to enhance the susceptibility of bacterial biofilms for surface disinfection. In vitro studies have demonstrated that the ionic silver treatment of 60 min significantly increased the logarithmic reduction (LR) of bacterial populations on disinfectant-treated substrates and the electrical treatment enhanced the silver susceptibility of E. coli biofilms. The LR values after the ionic silver treatments and the electric-enhanced silver treatments were in the ranges of 1.94–2.25 and 2.10–2.73, respectively. The treatment was also associated with morphological changes in silver-treated E. coli cells and biofilm-contaminated titanium surfaces. Nevertheless, the treatments showed no cytotoxic effects on the L929 mouse skin fibroblast cell line and only a slight decrease in pH was observed during the electrical polarization of titanium substrate.

Multifunctional Cellulosic Natural Rubber and Silver Nanoparticle Films with Superior Chemical Resistance and Antibacterial Properties

“…Besides acting as a pre-agglomeration stabilizer to obtain high dispersion of the primary cellulose filler in NRL, SCMC is also a stabilizer of silver nanoparticles (AgNPs) [ 17 ], which is adopted to enhance antimicrobial property to NR composites in this study. AgNP-based biopolymers offer good biocompatibility and biodegradability and can be considered as an effective material for application in antimicrobial packaging and pharmaceutical and medical fields [ 18 , 19 , 20 , 21 ]. The antimicrobial effect of Ag has been previously demonstrated against many types of microorganisms, including bacteria, viruses, and protozoa [ 18 , 19 , 20 , 21 ].…”

“…AgNP-based biopolymers offer good biocompatibility and biodegradability and can be considered as an effective material for application in antimicrobial packaging and pharmaceutical and medical fields [ 18 , 19 , 20 , 21 ]. The antimicrobial effect of Ag has been previously demonstrated against many types of microorganisms, including bacteria, viruses, and protozoa [ 18 , 19 , 20 , 21 ]. It has been shown that AgNPs exhibit strong antibacterial activities against both Gram-positive and Gram-negative bacteria.…”

Analysis and Optimization of the Effect of Process Parameters of Silver Anode Technique on Pseudomonas aeruginosa in Raw Milk

“…Suttasattakrit and coauthors [19] determined that the applications of electric currents creates a synergistic effect on the antibacterial effect of silver. The researchers obtained an effective antibacterial and antibiofilm result compared to the model without ionic silver and current.…”