Iopromide in combination with IFN-γ induces the activation of HMC-1 cells via IL-4 and MCP-1 expression

Cho, Hae-Yun; Choi, Seok Jin; Lee, Soo-Woon; Kim, Yang Weon; Lee, Chae Kwan; Lee, Soo-Woong

doi:10.1016/j.cellimm.2014.12.010

Cited by 2 publications

(3 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have indicated that apoptosis of endothelial cells is induced by high-dose CM; however, the exposure time of high-dose CM was a very short period. Petersein et al 24) had found that only 76% of the administered dose (250 mg total iodine/kg body weight) in vivo was eliminated via the urine at 24 h, whereas concentrations in liver, spleen and blood were still remained 7.7, 2.5, and 6.3%, respectively. Therefore, we first decided the low-dose CM, which did not affect the viability of endothelial cells, with various nonionic iodinated contrast media, including iobitridol, iohexol, iobrix, and Ultravist.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we selected Ultravist for use in the subsequent experiments because Ultravist is a widely used pharmaceutical agent. 25) It is well known that endothelial cells are directly affected by various cytokines and chemokines in a variety of disease conditions. We also found that Ultravist-stimulated human mast cells (HMC-1) express a lot of cytokines and chemokines, including IL-4, IL-6, TNF-α, and MCP-1 in the previous study.…”

Section: Discussionmentioning

confidence: 99%

“…We also found that Ultravist-stimulated human mast cells (HMC-1) express a lot of cytokines and chemokines, including IL-4, IL-6, TNF-α, and MCP-1 in the previous study. 25) Thus, we next investigated the effects of cytokines, including TNF-α, IFN-γ, IL-4, and IL-6, but found that only IL-4 affected the function of endothelial cells (data not shown).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Ultravist Induces the Expression of MCP-1 and VCAM-1 in IL-4-Stimulated HUVECs

Kim

Cho

et al. 2019

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

The goal of the present study focused on the adverse reaction of contrast medium (CM) via the induction of inflammatory molecules in human umbilical vein endothelial cells (HUVECs). Ultravist-induced monocyte chemoattractant protein-1 (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1) gene expression was markedly increased in interleukin-4 (IL-4)-pretreated HUVECs in a time-and dose-dependent manner and was paralleled by concomitant production of MCP-1 and VCAM-1 proteins. MCP-1 and VCAM-1 gene expression by Ultravist in combination with IL-4 was mediated by the c-Jun N-terminal kinases (JNK1/2) signaling pathway. IL-4-pretreated Ultravist-stimulated HUVECs showed greatly increased migration and adhesion of THP-1 cells. Cell migration was decreased by treatment of CCR2 antagonist, and cell adhesion wasalso decreased by VCAM-1 blocking antibody. Furthermore, when tested in vivo under similar conditions, MCP-1 protein was significantly increased in Ultravist combined with IL-4-injected mice. Taken together, our findings suggest that MCP-1 blocking may be crucial in preventing the endothelial dysfunction induced by contrast medium in patients with inflammatory disease and atherosclerosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ultravist Induces the Expression of MCP-1 and VCAM-1 in IL-4-Stimulated HUVECs

Kim

Cho

et al. 2019

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon

et al. 2018

View full text Add to dashboard Cite

Chagas disease is an infection caused by the parasite Trypanosoma cruzi that affects millions of people worldwide and is endemic in Latin America. Megacolon is the most frequent complication of the digestive chronic form and happens due to lesions of the enteric nervous system. The neuronal lesions seem to initiate in the acute phase and persist during the chronic phase, albeit the mechanisms involved in this process are still debated. Among the cells of the immune system possibly involved in this pathological process is the mast cell (MC) due to its well-known role in the bi-directional communication between the immune and nervous systems. Using ultrastructural analysis, we found an increased number of degranulated MCs in close proximity to nerve fibers in infected patients when compared with uninfected controls. We also immunostained MCs for the two pro-inflammatory molecules tryptase and chymase, the first being also important in neuronal death. The number of MCs immunostained for tryptase or chymase was increased in patients with megacolon, whereas increased tryptase staining was additionally observed in patients without megacolon. Moreover, we detected the expression of the tryptase receptor PAR2 in neurons of the enteric nervous system, which correlated to the tryptase staining results. Altogether, the data presented herein point to the participation of MCs on the denervation process that occurs in the development of T. cruzi-induced megacolon.

show abstract

Iopromide in combination with IFN-γ induces the activation of HMC-1 cells via IL-4 and MCP-1 expression

Cited by 2 publications

References 36 publications

Ultravist Induces the Expression of MCP-1 and VCAM-1 in IL-4-Stimulated HUVECs

Ultravist Induces the Expression of MCP-1 and VCAM-1 in IL-4-Stimulated HUVECs

Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon

Contact Info

Product

Resources

About