iPAINT: a general approach tailored to image the topology of interfaces with nanometer resolution

Aloi, Antonio; Vilanova, Neus; Albertazzi, Lorenzo; Voets, Ilja K.

doi:10.1039/c6nr00445h

Cited by 49 publications

(51 citation statements)

References 28 publications

(39 reference statements)

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“…39 A very recent study introduced a new technique based on TIRF and photo-activatable dyes to analyze interfaces supporting nanobubbles. 40 An alternative approach reported by Karpitschka et al analyzed the nucleation of surface nanobubbles on glass by label-free optical interference-enhanced reflection microscopy and showed co-localization of alleged surface nanobubbles by optical and atomic force microscopy. 41 Finally, the change in fluorescence properties of a surface immobilized dye was shown to allow one to localize nanobubbles.…”

Section: Introductionmentioning

confidence: 99%

Surface Nanobubbles Studied by Time-Resolved Fluorescence Microscopy Methods Combined with AFM: The Impact of Surface Treatment on Nanobubble Nucleation

et al. 2016

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The impact of surface treatment and modification on surface nanobubble nucleation in water has been addressed by a new combination of fluorescence lifetime imaging microscopy (FLIM) and atomic force microscopy (AFM). In this study, rhodamine 6G (Rh6G)-labeled surface nanobubbles nucleated by the ethanol-water exchange were studied on differently cleaned borosilicate glass, silanized glass as well as self-assembled monolayers on transparent gold by combined AFM-FLIM. While the AFM data confirmed earlier reports on surface nanobubble nucleation, size, and apparent contact angles in dependence of the underlying substrate, the colocalization of these elevated features with highly fluorescent features observed in confocal intensity images added new information. By analyzing the characteristic contributions to the excited state lifetime of Rh6G in decay curves obtained from time-correlated single photon counting (TCSPC) experiments, the characteristic short-lived (<600 ps) component of could be associated with an emission at the gas-water interface. Its colocalization with nanobubble-like features in the AFM height images provides evidence for the observation of gas-filled surface nanobubbles. While piranha-cleaned glass supported nanobubbles, milder UV-ozone or oxygen plasma treatment afforded glass-water interfaces, where no nanobubbles were observed by combined AFM-FLIM. Finally, the number density of nanobubbles scaled inversely with increasing surface hydrophobicity.

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Section: Introductionmentioning

confidence: 99%

Surface Nanobubbles Studied by Time-Resolved Fluorescence Microscopy Methods Combined with AFM: The Impact of Surface Treatment on Nanobubble Nucleation

et al. 2016

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“…An area in which fluorescence nanoscopy has the potential to make an impact is in the study of interfaces in soft materials. Recently, a novel method was developed that allows nanoscale imaging of solid/liquid, liquid/liquid, and liquid/air interfaces, in a modification of the PAINT technique . The noninvasiveness of this method and the ability to study 3D dynamic interfaces holds promise in the study of complex soft materials.…”

Section: Discussionmentioning

confidence: 99%

Super‐resolution Fluorescence Imaging for Materials Science

2017

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While super‐resolution fluorescence imaging has mostly seen applications in the life sciences, an increasing number of laboratories are using these techniques to study materials. This often requires adaptation of the more commonly employed protocols that have been developed for biological systems. Here, the most representative examples of the use of super‐resolution fluorescence microscopy to study a wide range of materials, including polymers, nanofibers, carbon nanostructures, inorganic materials, and other nonbiological systems, are collected. Due to its ability to provide dynamic information, to probe below the outer surface of a material, and to gain information at the molecular level beyond ensemble averaging, super‐resolution fluorescence microscopy has the potential to provide new insights that complement those obtained by well‐established techniques in materials science.

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“…In particular, point accumulation for imaging in nanoscale topography (PAINT) modalities have emerged as a powerful and versatile technique to reconstruct superresolved images of biomolecular structures and cell membranes. [15][16][17][18] This technique relies on the super-localization of individual fluorophores bound to the target of interest. These…”

Section: Doi: 101002/smll201902796mentioning

confidence: 99%

NanoPaint: A Tool for Rapid and Dynamic Imaging of Membrane Structural Plasticity at the Nanoscale

Tasso

Pons

Lequeux

et al. 2019

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Single‐particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano‐resolved population dynamics of QDs is exploited to reconstruct the topography and structural changes of the cell membrane surface with high temporal and spatial resolution. For this proof‐of‐concept study, bright, small, and stable biofunctional QD nanoconstructs are utilized recognizing the endogenous neuronal cannabinoid receptor 1, a highly expressed and fast‐diffusing membrane protein, together with a commercial point‐localization microscope. Rapid QD diffusion on the axonal plasma membrane of cultured hippocampal neurons allows precise reconstruction of the membrane surface in less than 1 min with a spatial resolution of tens of nanometers. Access of the QD nanoconstructs to the synaptic cleft enables rapid 3D topological reconstruction of the entire presynaptic component. Successful reconstruction of membrane nano‐topology and deformation at the second time‐scale is also demonstrated for HEK293 cell filopodia and axons. Named “nanoPaint,” this super‐resolution imaging technique amenable to any endogenous transmembrane target represents a versatile platform to rapidly and accurately reconstruct the cell membrane nano‐topography, thereby enabling the study of the rapid dynamic phenomena involved in neuronal membrane plasticity.

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iPAINT: a general approach tailored to image the topology of interfaces with nanometer resolution

Abstract: iPAINT enables three-dimensional super-resolution imaging of soft and deformable interfaces in nanomaterials without the need of covalent labelling.

Cited by 49 publications

References 28 publications

Surface Nanobubbles Studied by Time-Resolved Fluorescence Microscopy Methods Combined with AFM: The Impact of Surface Treatment on Nanobubble Nucleation

Surface Nanobubbles Studied by Time-Resolved Fluorescence Microscopy Methods Combined with AFM: The Impact of Surface Treatment on Nanobubble Nucleation

Super‐resolution Fluorescence Imaging for Materials Science

NanoPaint: A Tool for Rapid and Dynamic Imaging of Membrane Structural Plasticity at the Nanoscale

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