IPAP: Image Processor for Analytical Pathology.

Watanabe, Tomoyuki; Katsura, Yasunori; Yoshitake, Akira; Masataki, Hirokazu; Mori, Takeshi

doi:10.1293/tox.7.353

Cited by 58 publications

(27 citation statements)

References 8 publications

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“…Image Analysis (Experiments 2A,2B,and 2C) Each section stained immunohistochemically was examined with the assistance of an image analyzer (IPAP; Image Processor for Analytical Pathology, Sumika Technoservice Co., Hyogo, Japan) (Watanabe et al 1994). More than twenty microscope images (400X) from all lung lobes of the rats were assessed for numbers and areas of immunohistochemically positive cells.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Lung Toxicity of 16 Fine Particles on Intratracheal Instillation in a Bioassay Model Using F344 Male Rats

et al. 2008

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We have developed a bioassay model to estimate toxicity of fine particles in the lungs at an early stage after intratracheal instillation (Yokohira et al. 2005;Yokohira et al. 2007). The present experiment was conducted to improve the model by estimating appropriate doses based on dose-dependent toxicity of instilled quartz (4 mg to 0 mg) as a positive control and assessing the impact of powdered particles without suspension (Experiment 1). In addition, examination of the toxicity of a series of particles was performed with the developed bioassay (Experiments 2A, 2B, and 2C). The materials chosen were sixteen particles, including nanoparticles and diesel powder. Histopathological and immunohistochemical analysis of bromodeoxyuridine (BrdU) incorporation and inducible nitric oxide synthase (iNOS) were performed after exposure of the lungs.A dose of 2 mg quartz suspended in 0.2 mL saline was suggested to be most appropriate for sensitive detection of acute and subchronic inflammatory changes. Although some materials, including nanoparticles, demonstrated toxicity that was too strong for sensitive assessment, the ranking order could be given as follows: CuO > quartz > neutralized Na 2 PdCl 4 > NiO > hydrotalcite > MnO 2 > diesel > titanium dioxide (in Experiment 2B) > β-cyclodextrin > diesel standard > titanium dioxide (in Experiment 2A) > CaCO 3 .

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Section: Immunohistochemical Analysismentioning

confidence: 99%

Lung Toxicity of 16 Fine Particles on Intratracheal Instillation in a Bioassay Model Using F344 Male Rats

et al. 2008

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“…For quantitative analysis, immunostained sections were examined under a light microscope connected to an image analysis system, Image Processor for Analytical Pathology (IPAP; Sumika Technos Corp., Osaka). 8) Binary digitized images of liver lesions were obtained automatically by the programmed segmentation procedure. The length of positive cell surface for pan-cadherin staining was measured at a magnification of 600 (at least 10 fields) for each lesion.…”

Section: Quantitative Analysismentioning

confidence: 99%

Establishment of an in vivo Highly Metastatic Rat Hepatocellular Carcinoma Model

Futakuchi

Hirose

Ogiso

et al. 1999

Japanese Journal of Cancer Research

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We previously found by chance that N-nitrosomorpholine (NMOR) given after a multi-carcinogenic treatment induces liver carcinomas with 56% lung metastasis, and it was confirmed that hepatocellular carcinoma (HCC) with 100% lung metastasis was produced by 24-week treatment with NMOR and additional treatment with diethylnitrosamine (DEN). In the present study, we modified the duration of NMOR to establish an animal model with a simple experimental protocol and an appropriate experimental duration which would facilitate further study of the mechanisms of metastasis and antimetastatic agents. The results revealed DEN exposure followed by a 16-week treatment with NMOR to be a most efficient method for the induction of HCC metastasizing to the lung. Loss of cadherin, demonstrated immunohistochemically, occurred in an early stage of carcinogenesis, and this was reflected in malignant conversion of primary lesions. This model, with its essential similarities to malignant tumor behavior in man, should find application not only for elucidation of the mechanisms underlying metastasis, but also in the development of anti-metastatic agents. Key words: Rat hepatocarcinogenesis model -Lung metastasis -N-Nitrosomorpholine -CadherinTo form distant metastases, a sequence of many steps, including invasion, transport, arrest, adherence, extravasation, and tumor cell proliferation is required. 1) To study the mechanisms underlying metastasis, many tools and models have been developed. Most of them use cancer cell lines or transplantable tumors, injected into blood vessels or the intraperitoneal cavity, or transplanted into the cecum, spleen or subcutis.2-6) These models have provided very useful tools for analysis of individual steps in the metastatic process. However, in order to analyze the natural course of metastasis, it is necessary to develop animal cancer models which feature frequent metastasis of primary tumors to distant organs. They are also required to assess the efficacy of therapeutic treatments for advanced cancers.We previously found by chance that N-nitrosomorpholine (NMOR) given after a multi-carcinogenic treatment with N-diethylnitrosamine (DEN), N-methylnitrosourea (MNU), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN), 1,2-dimethylhydrazine (DMH), and 2,2′-dihydroxy-di-Npropylnitrosamine (DHPN) induces liver carcinomas with frequent lung metastasis. 7)The purpose of the present study was to establish an animal model with a simple experimental protocol and an appropriate experimental duration which would facilitate further study of the mechanisms of metastasis and antimetastatic agents. We also examined cadherin expression in primary lesions by immunohistochemistry.

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“…Five microscopic fields at 250ϫ magnification were randomly chosen in Picrosirius red-stained sections, and the fibrotic area per unit square was measured using a color image processor (IPAP; Sumica Technos, Osaka, Japan). 45 Immunohistochemistry. Another part of PLP-fixed materials was embedded in OCT compound (Sakura Fine Technical Co., Tokyo, Japan) after successive transfer in 8% to 20% sucrose in 0.1 mol/L phosphate buffer (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Hepatic Stellate Cell Proliferation and Activation by the Semisynthetic Analogue of Fumagillin Tnp–470 in Rats

et al. 2000

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IPAP: Image Processor for Analytical Pathology.

Cited by 58 publications

References 8 publications

Lung Toxicity of 16 Fine Particles on Intratracheal Instillation in a Bioassay Model Using F344 Male Rats

Lung Toxicity of 16 Fine Particles on Intratracheal Instillation in a Bioassay Model Using F344 Male Rats

Establishment of an in vivo Highly Metastatic Rat Hepatocellular Carcinoma Model

Inhibition of Hepatic Stellate Cell Proliferation and Activation by the Semisynthetic Analogue of Fumagillin Tnp–470 in Rats

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