IPNV modulation of pro and anti-inflammatory cytokine expression in Atlantic salmon might help the establishment of infection and persistence

Reyes-Cerpa, Sebastián; Reyes-López, Felipe E.; Toro-Ascuy, Daniela; Ibáñez, Jorge; Maisey, Kevin; Sandino, Ana María; Imarai, Mónica

doi:10.1016/j.fsi.2011.11.018

Cited by 45 publications

(36 citation statements)

References 59 publications

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“…In the present work, the increase in IL-4, IgG and IgM as well as the decrease in pro-inflammatory cytokines IL-1α and IL-2 implies the favorable effect of soy phytoestrogens on Immunity as they improved general humeral immune response and prevented autoimmunity as IL-4 stimulated signaling array in B cells [26]. Moreover, inhibition of IL-2 production led to the inhibition of Th1 and enhancement of Th2 cytokine synthesis in CD4+ T cells [27].…”

Section: Discussionsupporting

confidence: 51%

Influence of Dietary Soy Phytoestrogens on Cytokine Production and Immunoglobulin in Ovariectomized Rats

2016

IJACEBS

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Abstract-This study was designed to examine the relationship between soy phytoestrogens supplementation in ovariectomized rats and pro-inflammatory cytokines Interleukin 1 alpha (IL-1α), Interleukin 2 (IL-2), Interleukin 4 (IL-4) and immunoglobulin. Twenty four female rats were ovariectomized then divided equally into 3 groups; control group, was fed on casein based diet, low soy phytoestrogens group was fed a diet contained 7% soy and high soy phytoestrogens group received a diet of 26% soy for 28 days. At the end of experiment, rats were weighed then blood samples were collected on EDTA for total leukocyte count (TLC). Serum samples were taken for interleukins assay, lysozyme activity and immunoglobulin. Animals were sacrificed and thymus and spleen were weighed. The body weight gain was significantly declined in ovariectomized rats fed high soy diet at the first 3 weeks of the experiment. Rats fed high soy diet showed significant reduction in thymic weight compared to control ones. IL-1α and IL-2 were significantly suppressed in rats fed high soy diet compared to control rats. However, IL-4 was significantly stimulated in rats fed high soy diet compared to control rats. Both IgM and IgG levels were significantly increased in rats fed high soy diet compared to control rats. There was a significant decrease in lysozyme activity in rats fed high soy diet compared to rats fed low soy diet. TLC was significantly increased in rats fed high and low soy diets. In conclusion, soy supplementation altered selected immunological parameters of ovariectomized female rats.

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Section: Discussionsupporting

confidence: 51%

Influence of Dietary Soy Phytoestrogens on Cytokine Production and Immunoglobulin in Ovariectomized Rats

2016

IJACEBS

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“…IL-10 has been identified and characterized in various fish species such as zebrafish Danio rerio [49], rainbow trout [50], and sea bass [51]. In Atlantic salmon, up-regulation of IL-10 was observed after LPS and poly(I:C) injection at 24 hpi [45]. In this study, we observed the significant up-regulation of IL-10 in all species treated with FKC of V. anguillarum.…”

Section: Discussionsupporting

confidence: 58%

“…FKC of V. anguillarumtreated Atlantic cod up-regulated IL-1β at 24 hpi [26]. Upregulation of IL-1β was also observed in poly(I:C)-treated Atlantic salmon Salmo salar at 24 hpi [45].…”

Section: Discussionmentioning

confidence: 81%

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Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Jirapongpairoj

Kobayashi

Fukuda³

et al. 2015

Fish Sci

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“…The PCR products were visualized by electrophoresis in a 2% agarose gel. To demonstrate that RNA inputs had similar levels, salmon eF1a was used as a housekeeping gene (39). Modeling the HE protein of ISAV901_09 with GFP inserted within the HPR region.…”

Section: Methodsmentioning

confidence: 99%

Development of a Reverse Genetic System for Infectious Salmon Anemia Virus: Rescue of Recombinant Fluorescent Virus by Using Salmon Internal Transcribed Spacer Region 1 as a Novel Promoter

Toro-Ascuy

Tambley

Beltrán

et al. 2015

Appl Environ Microbiol

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Infectious salmon anemia (ISA) is a serious disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), belonging to the genus Isavirus, family Orthomyxoviridae. There is an urgent need to understand the virulence factors and pathogenic mechanisms of ISAV and to develop new vaccine approaches. Using a recombinant molecular biology approach, we report the development of a plasmid-based reverse genetic system for ISAV, which includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). Salmon cells cotransfected with pSS-URG-based vectors expressing the eight viral RNA segments and four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex allowed the generation of infectious recombinant ISAV (rISAV). We generated three recombinant viruses, wild-type rISAV 901_09 and rISAVr S6-NotI-HPR containing a NotI restriction site and rISAV S6/EGFP-HPR harboring the open reading frame of enhanced green fluorescent protein (EGFP), both within the highly polymorphic region (HPR) of segment 6. All rescued viruses showed replication activity and cytopathic effect in Atlantic salmon kidney-infected cells. The fluorescent recombinant viruses also showed a characteristic cytopathic effect in salmon cells, and the viruses replicated to a titer of 6.5 ؋ 10 5 PFU/ml, similar to that of the wild-type virus. This novel reverse genetics system offers a powerful tool to study the molecular biology of ISAV and to develop a new generation of ISAV vaccines to prevent and mitigate ISAV infection, which has had a profound effect on the salmon industry. Infectious salmon anemia (ISA) is a disease that principally affects Atlantic salmon (Salmo salar), which has caused enormous losses to salmon farming worldwide (1, 2). ISA was first reported in 1984 in Norway (3) and subsequently was identified in Canada (4), Scotland (5), the United States (6), the Faroe Islands (Denmark) (6), and Chile (7). The etiological agent of this disease is the infectious salmon anemia virus (ISAV), a pleomorphic enveloped virus with a diameter of 45 to 140 nm, which belongs to the family Orthomyxoviridae (8). Its genome has 8 negative-polarity singlestranded RNA (ssRNA) segments that encode 10 proteins. Sequence similarity analyses of the genomes of different ISAV isolates show significant variation in the coding regions. In contrast, the untranslated regions (UTR) at both ends of each segment are highly conserved (9-11), as has been described for other orthomyxoviruses as well (e.g., influenza A and B viruses) (12).There is little knowledge at present about the functions of the ISAV proteins. Bioinformatic analyses of the sequences predict that segments 1, 2, and 4 encode the subunits of the RNA-dependent RNA polymerase (RdRp), analogous to PB2, PB1, and PA, respectively, of the influenza A virus. Segment 3 encodes the nucleoprotein (NP), which has been reported to have the capacity to bind ssRNA (13). For influenza virus, it has been shown that t...

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IPNV modulation of pro and anti-inflammatory cytokine expression in Atlantic salmon might help the establishment of infection and persistence

Cited by 45 publications

References 59 publications

Influence of Dietary Soy Phytoestrogens on Cytokine Production and Immunoglobulin in Ovariectomized Rats

Influence of Dietary Soy Phytoestrogens on Cytokine Production and Immunoglobulin in Ovariectomized Rats

Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Development of a Reverse Genetic System for Infectious Salmon Anemia Virus: Rescue of Recombinant Fluorescent Virus by Using Salmon Internal Transcribed Spacer Region 1 as a Novel Promoter

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