IR-780-loaded polymeric micelles enhance the efficacy of photothermal therapy in treating breast cancer lymphatic metastasis in mice

He, Bin; Hu, Haiyan; Tan, Tao; Wang, Hong; Sun, Kaoxiang; Li, Yaping; Zhang, Zhiwen

doi:10.1038/aps.2017.109

Cited by 32 publications

(15 citation statements)

References 34 publications

(43 reference statements)

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“…Additionally, the critical micellar concentration (CMC) of the PCB-lipid was 127 µg/mL, suggesting that PCB-lipid micelles were formed at low concentration and could load hydrophobic drugs more efficiently ( Figure S3 ). The IR-780–PCB-lipid feed ratio was set to a 1:10 weight ratio, and therefore, the LC (%) and EE (%) values were obtained as 5.1 and 51.1, respectively, and they are comparatively higher than other micellar nanoparticle for IR-780 delivery [ 12 , 13 ]. The absorbance spectrum of the IR-780 dye loaded in the PCB-lipid–IR-780 NPs showed a 15-nm redshift to 803 nm, indicating that hydrophobic dye inside the micelles experienced a change in polarity and hydrophobic interactions inside the micelles; and such a redshift also demonstrated that IR-780 was efficiently loaded into the PCB-lipid micellar NPs rather than physically adsorbed onto the surface of the nanoparticles [ 7 , 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

A Lipophilic IR-780 Dye-Encapsulated Zwitterionic Polymer-Lipid Micellar Nanoparticle for Enhanced Photothermal Therapy and NIR-Based Fluorescence Imaging in a Cervical Tumor Mouse Model

Rajendrakumar

Chang

Mohapatra

et al. 2018

IJMS

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To prolong blood circulation and avoid the triggering of immune responses, nanoparticles in the bloodstream require conjugation with polyethylene glycol (PEG). However, PEGylation hinders the interaction between the nanoparticles and the tumor cells and therefore limits the applications of PEGylated nanoparticles for therapeutic drug delivery. To overcome this limitation, zwitterionic materials can be used to enhance the systemic blood circulation and tumor-specific delivery of hydrophobic agents such as IR-780 iodide dye for photothermal therapy. Herein, we developed micellar nanoparticles using the amphiphilic homopolymer poly(12-(methacryloyloxy)dodecyl phosphorylcholine) (PCB-lipid) synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerization. The PCB-lipid can self-assemble into micelles and encapsulate IR-780 dye (PCB-lipid–IR-780). Our results demonstrated that PCB-lipid–IR-780 nanoparticle (NP) exhibited low cytotoxicity and remarkable photothermal cytotoxicity to cervical cancer cells (TC-1) upon near-infrared (NIR) laser irradiation. The biodistribution of PCB-lipid–IR-780 showed higher accumulation of PCB-lipid–IR-780 than that of free IR-780 in the TC-1 tumor. Furthermore, following NIR laser irradiation of the tumor region, the PCB-lipid–IR-780 accumulated in the tumor facilitated enhanced tumor ablation and subsequent tumor regression in the TC-1 xenograft model. Hence, these zwitterionic polymer-lipid hybrid micellar nanoparticles show great potential for cancer theranostics and might be beneficial for clinical applications.

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Section: Discussionmentioning

confidence: 99%

A Lipophilic IR-780 Dye-Encapsulated Zwitterionic Polymer-Lipid Micellar Nanoparticle for Enhanced Photothermal Therapy and NIR-Based Fluorescence Imaging in a Cervical Tumor Mouse Model

Rajendrakumar

Chang

Mohapatra

et al. 2018

IJMS

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“…However, the characteristics of IR-780 iodide, such as poor water solubility, rapid clearance, and acute toxicity, limit its direct clinical use 19 , 20 . A good approach is to encapsulate IR-780 iodide in nanostructures and deliver it to tumor cells 21 – 23 . Notably, carrying IR-780 iodide within nanostructures does not require chemical bond modification, and the combination is convenient and reliable.…”

Section: Introductionmentioning

confidence: 99%

Preparation of multifunctional nanobubbles and their application in bimodal imaging and targeted combination therapy of early pancreatic cancer

Yang

Zhao

Zhou

et al. 2021

Sci Rep

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Pancreatic cancer will gradually become the second leading cause of cancer death due to its poor suitability for surgical treatment, frequent recurrence and metastasis, and insensitivity to radiotherapy and chemotherapy. Strategies for precise early detection and effective targeted treatment of pancreatic cancer are urgently needed. Because of its unique advantages, molecular targeted contrast-enhanced ultrasound imaging (CEUI) has generated new opportunities to overcome this challenge. The aim of this study was to explore multifunctional nanobubbles named IR780-NBs-DTX as novel ultrasound contrast agents (UCAs) for dual-mode targeted imaging and photothermal ablation combined with chemotherapy for pancreatic cancer. An optimized “film hydration method” was used to prepare IR780-NBs-DTX in this research. The characteristics and ability of the new UCAs were detected via in vitro, in vivo and ex vivo experiments. The initial dose of 0.15 mg IR-780 iodide/1.0 mg DTX was considered to be the best formula for IR780-NBs-DTX, and the concentration of 6 ×106 bubbles/mL was best for CEUI. The excellent characteristics of IR780-NBs-DTX, including a uniform nanoscale particle size (349.8± 159.1 nm, n= 3), good performance in dual-mode imaging, high stability and reliable biocompatibility, were also proven. In the in vitro cell experiments, IR780-NBs-DTX targeted more pancreatic cancer cells than the control treatments, and the targeting rate was approximately 95.6± 1.7%. Under irradiation with an 808 nm laser, most cells died. Furthermore, the in vivo study demonstrated that IR780-NBs-DTX could precisely detect pancreatic cancer through near infrared fluorescence (NIRF) imaging and CEUI, and the tumor almost disappeared at 18 days after combined treatment. In ex vivo experiments, immunohistochemistry (IHC) and immunofluorescence (IF) showed that the expression of HSP70 increased and that of PCNA decreased, and many apoptotic tumor cells were observed by TUNEL staining in the IR780-NBs-DTX group. The newly prepared IR780-NBs-DTX are novel nanosized UCAs with high efficiency for dual-mode molecular targeted imaging and combined therapy, and they may have future potential applications in the precise detection and effective targeted therapy of small and metastatic lesions in the early stage of pancreatic cancer.

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“…Even though there have been many nanocarriers like polymers, liposomes, and inorganic molecules for encapsulating PTT agents, most of them were not efficient in co‐loading with other chemo agents. To exemplify, Caixia Yue et al., (2013) reported that the IR‐780 dye loaded tumor targeting theranostic nanoparticles for NIR imaging and PTT in which loading therapeutics agents would be difficult as the carrier is made of hydrophilic heparin .…”

Section: Resultsmentioning

confidence: 99%

Olive Oil‐Based Ultrafine Theranostic Photo Nanoemulsions: A Versatile Tumor Maneuvering Nanoplatform for Precise Controlled Drug Release in Tumor and Complete Tumor Eradication Mediated by Photo‐Chemotherapy

Rejinold

Cherukula

et al. 2019

Advanced Therapeutics

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Photothermal therapy (PTT) is an efficient approach to treat tumors. However, heterogeneous distribution of heat by PTT in the tumor region often results in tumor regrowth and metastasis. Integrating PTT with chemotherapy in a precisely controlled tumor maneuvering and drug-releasing nanoconstruct will be an ideal strategy for higher therapeutic index. Herein, nanoemulsions (NEMSn) based on a highly biocompatible extra virgin olive oil are developed to encapsulate IR-780 for PTT and near infrared fluorescence imaging as well as doxorubicin (DOX) for chemotherapy. NEMSn are further stabilized with Tween-20 and hyaluronic acid to form an ultrafine theranostic photo nanoemulsions (UTPNEMSn) for combinatorial photo-chemotherapy. Once injected into the system, UTPNEMS homes to the tumor region owing to passive targeting. Tumor accumulated UTPNEMSn readily destabilizes upon laser irradiation to release the DOX, thereby synergizing the therapeutic effects of DOX and PTT. In vivo studies demonstrate a complete tumor eradication without tumor recurrence or systemic toxicity, owing to their biocompatible formulation. By virtue of the olive oil based NEMSNs and precise controlled drug releasing properties collaborating with the synergistic therapeutic modalities, UTPNEMSn are able to provide a promising method for controlled drug delivery systems.

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IR-780-loaded polymeric micelles enhance the efficacy of photothermal therapy in treating breast cancer lymphatic metastasis in mice

Cited by 32 publications

References 34 publications

A Lipophilic IR-780 Dye-Encapsulated Zwitterionic Polymer-Lipid Micellar Nanoparticle for Enhanced Photothermal Therapy and NIR-Based Fluorescence Imaging in a Cervical Tumor Mouse Model

A Lipophilic IR-780 Dye-Encapsulated Zwitterionic Polymer-Lipid Micellar Nanoparticle for Enhanced Photothermal Therapy and NIR-Based Fluorescence Imaging in a Cervical Tumor Mouse Model

Preparation of multifunctional nanobubbles and their application in bimodal imaging and targeted combination therapy of early pancreatic cancer

Olive Oil‐Based Ultrafine Theranostic Photo Nanoemulsions: A Versatile Tumor Maneuvering Nanoplatform for Precise Controlled Drug Release in Tumor and Complete Tumor Eradication Mediated by Photo‐Chemotherapy

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