IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

Bokhart, Mark T.; Manni, Jeffrey G.; Garrard, Kenneth P.; Ekelöf, Måns; Nazari, Milad; Muddiman, David C.

doi:10.1007/s13361-017-1740-x

Cited by 41 publications

(48 citation statements)

References 31 publications

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“…The choice of the matrix is not as critical as in with MALDI since ice can be used universally for different tissue types and analytes without the drawback of matrix‐related peaks in the lower m / z region of the spectra (Robichaud et al, 2013; Nazari et al, 2018). IR‐MALDESI also offers optimal spatial resolution up to 50 µm (Bokhart et al, 2017)…”

Section: Msi Methodsmentioning

confidence: 99%

The Space Dimension at the Micro Level: Mass Spectrometry Imaging of Drugs in Tissues

Davoli

Zucchetti

Matteo

et al. 2020

Mass Spectrometry Reviews

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Mass spectrometry imaging (MSI) has seen remarkable development in recent years. The possibility of getting quantitative or semiquantitative data, while maintaining the spatial component in the tissues has opened up unique study possibilities. Now with a spatial window of few tens of microns, we can characterize the events occurring in tissue subcompartments in physiological and pathological conditions. For example, in oncology—especially in preclinical models—we can quantitatively measure drug distribution within tumors, correlating it with pharmacological treatments intended to modify it. We can also study the local effects of the drug in the tissue, and their effects in relation to histology. This review focuses on the main results in the field of drug MSI in clinical pharmacology, looking at the literature on the distribution of drugs in human tissues, and also the first preclinical evidence of drug intratissue effects. The main instrumental techniques are discussed, looking at the different instrumentation, sample preparation protocols, and raw data management employed to obtain the sensitivity required for these studies. Finally, we review the applications that describe in situ metabolic events and pathways induced by the drug, in animal models, showing that MSI makes it possible to study effects that go beyond the simple concentration of the drug, maintaining the space dimension. © 2020 John Wiley & Sons Ltd. Mass Spec Rev

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Section: Msi Methodsmentioning

confidence: 99%

The Space Dimension at the Micro Level: Mass Spectrometry Imaging of Drugs in Tissues

Davoli

Zucchetti

Matteo

et al. 2020

Mass Spectrometry Reviews

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“…Heatmaps and histogram of MMA of endogenous GSH in both tissues (Figure S1) were generated using the built-in MMA Heatmap function in MSiReader. 35…”

Section: Methodsmentioning

confidence: 99%

Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Nazari

Bokhart

Loziuk

et al. 2018

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A quantitative mass spectrometry imaging (QMSI) method for absolute quantification of glutathione (GSH) in healthy and cancerous hen ovarian tissues using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is presented. Using this technique, the ion abundance of GSH was normalized to that of a structural analogue, which was sprayed on the slide prior to mounting the tissue sections. This normalization strategy significantly improved the voxel-to-voxel variability; the variability is attributed to the overall ionization process. Subsequently, a series of calibration spots of stable isotope-labeled (SIL) GSH were pipetted on top of the tissue to construct a spatial calibration curve, and calculate the concentration of GSH in both tissue sections. The QMSI results were verified by LC-MS/MS quantification of GSH for the same tissues. GSH was extracted from tissue sections in a slightly acidic buffer and was then alkylated using N-ethylmaleimide to minimize autoxidation of GSH to glutathione disulfide. The alkylated GSH was separated from other contaminants using reversed phase liquid chromatography (RPLC) coupled to a triple quadrupole mass spectrometer, and the z-ion transition of NEM-GSH was used to quantify GSH in each tissue section. While the absolute values obtained using IR-MALDESI QMSI and LC-MS/MS were different, a ∼2-fold increase in the concentration of GSH in cancer tissue compared to the healthy tissue was observed using both techniques. Possible reasons for the difference between absolute concentration values obtained using IR-MALDESI QMSI and LC-MS/MS are also discussed.

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“… 163 Agtuca et al explored the metabolic asymmetry between infected and noninfected soybean nodules with LAESI where elevated fatty acid, purine, and lipid levels occurred as a result of Bradyrhizobium japonicum infection. 164 The Muddiman group used ice matrices to increase lipid yields, 165 explored dynamic neurotransmitter imaging, 166 used IR-MALDESI for imaging metabolomics in plant and mammalian samples, 167 , 168 and coupled a drift tube for ion mobility direct collision cross section (CCS) measurements from several tissue types. 169 Most recently, cell-to-cell lipid heterogeneity in a dispersed HeLa cell culture was demonstrated by Xing et al with IR-MALDESI.…”

Section: Advances In Single-cell Ms Analysesmentioning

confidence: 99%

Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics

Taylor

Lukowski

Anderton

2021

J. Am. Soc. Mass Spectrom.

215

153

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Biological systems are composed of heterogeneous populations of cells that intercommunicate to form a functional living tissue. Biological function varies greatly across populations of cells, as each single cell has a unique transcriptome, proteome, and metabolome that translates to functional differences within single species and across kingdoms. Over the past decade, substantial advancements in our ability to characterize omic profiles on a single cell level have occurred, including in multiple spectroscopic and mass spectrometry (MS)-based techniques. Of these technologies, spatially resolved mass spectrometry approaches, including mass spectrometry imaging (MSI), have shown the most progress for single cell proteomics and metabolomics. For example, reporter-based methods using heavy metal tags have allowed for targeted MS investigation of the proteome at the subcellular level, and development of technologies such as laser ablation electrospray ionization mass spectrometry (LAESI-MS) now mean that dynamic metabolomics can be performed in situ. In this Perspective, we showcase advancements in single cell spatial metabolomics and proteomics over the past decade and highlight important aspects related to high-throughput screening, data analysis, and more which are vital to the success of achieving proteomic and metabolomic profiling at the single cell scale. Finally, using this broad literature summary, we provide a perspective on how the next decade may unfold in the area of single cell MS-based proteomics and metabolomics.

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IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

Cited by 41 publications

References 31 publications

The Space Dimension at the Micro Level: Mass Spectrometry Imaging of Drugs in Tissues

The Space Dimension at the Micro Level: Mass Spectrometry Imaging of Drugs in Tissues

Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics

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