IR thermocycler for centrifugal microfluidic platform with direct on-disk wireless temperature measurement system

Burger, Jürgen; Gross, André; Mark, Daniel; Roth, G.; Stetten, Felix von; Zengerle, Roland

doi:10.1117/12.887178

Cited by 7 publications

(7 citation statements)

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“…Burger et al used an IR thermocycler with an integrated on-disc wireless temperature system to improve noncontact heating. 132 The group obtained a heating rate of 5 °C/s with a proportional-integral-derivative (PID) controller by optimizing the disc materials and depth of the sample-holding cavity.…”

Section: Thermocycling For Na Amplificationmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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Section: Thermocycling For Na Amplificationmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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“…22 However, the spinning step will be interrupted, thus imposing serious limitation on the functional capability of the device. Other ways such as infrared (IR) irradiation 23,24 or hot air chamber 25 also cannot realize localized heating. Clearly, the combination of centrifugal forces for fluid actuation with continuous supply of on-chip electricity can offer a multitude of application opportunities.…”

Section: Power To Disc For Localized Heatingmentioning

confidence: 99%

“…22 The spinning step will be interrupted, thus imposing serious limitations on the functional capability of the device. Other ways such as infrared (IR) 23,24 or hot air chamber 25 cannot realize localized heating, which is very important for miniaturized compact devices. In addition, in situ active valving and temperature measurement also require electricity.…”

Section: Introductionmentioning

confidence: 99%

A lab-in-a-droplet bioassay strategy for centrifugal microfluidics with density difference pumping, power to disc and bidirectional flow control

Wang

Yang

et al. 2013

Lab Chip

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In this paper, we present a lab-in-a-droplet bioassay strategy for a centrifugal microfluidics or lab-on-a-disc (LOAD) platform with three important advancements including density difference pumping, power to disc and bidirectional flow control. First, with the water based bioassay droplets trapped in a micro-channel filled with mineral oil, centrifugal force due to the density difference between the water and oil phases actuates droplet movement while the oil based medium remains stationary. Second, electricity is coupled to the rotating disc through a split-core transformer, thus enabling on-chip real-time heating in selected areas as desired and wireless programmable functionality. Third, an inertial mechanical structure is proposed to achieve bidirectional flow control within the spinning disc. The droplets can move back and forth between two heaters upon changing the rotational speed. Our platform is an essential and versatile solution for bioassays such as those involving DNA amplification, where localized temperature cycling is required. Finally, without the loss of generality, we demonstrate the functionality of our platform by performing real-time polymerase chain reaction (RT-PCR) in a linear microchannel made with PTFE (Teflon) micro-tubing.

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“…For such on-chip temperature measurements, thin-film techniques to apply Pt-based RTDs [11,49,12] or thermocouples [50][51][52] were presented; however, all these approaches use silicon or glass substrates and non-standard thermocouple materials. For polymer-based microfluidics, the integration of a commercial thermistor was presented [53]. Other approaches take an advantage of temperature-dependent properties of certain measurement fluids, e.g., measured by color change [54] or fluorescence [56].…”

Section: On-chip Thermocouplementioning

confidence: 99%

Laser micromachining as a metallization tool for microfluidic polymer stacks

Brettschneider

Dorrer

Czurratis

et al. 2013

J. Micromech. Microeng.

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A novel assembly approach for the integration of metal structures into polymeric microfluidic systems is described. The presented production process is completely based on a single solid-state laser source, which is used to incorporate metal foils into a polymeric multi-layer stack by laser bonding and ablation processes. Chemical reagents or glues are not required. The polymer stack contains a flexible membrane which can be used for realizing microfluidic valves and pumps. The metal-to-polymer bond was investigated for different metal foils and plasma treatments, yielding a maximum peel strength of R ps = 1.33 N mm −1 . A minimum structure size of 10 μm was determined by 3D microscopy of the laser cut line. As an example application, two different metal foils were used in combination to micromachine a standardized type-T thermocouple on a polymer substrate. An additional laser process was developed which allows metal-to-metal welding in close vicinity to the polymer substrate. With this process step, the reliability of the electrical contact could be increased to survive at least 400 PCR temperature cycles at very low contact resistances.

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IR thermocycler for centrifugal microfluidic platform with direct on-disk wireless temperature measurement system

Cited by 7 publications

References 0 publications

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

A lab-in-a-droplet bioassay strategy for centrifugal microfluidics with density difference pumping, power to disc and bidirectional flow control

Laser micromachining as a metallization tool for microfluidic polymer stacks

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