2017
DOI: 10.3389/fimmu.2017.01525
|View full text |Cite
|
Sign up to set email alerts
|

iRAGu: A Novel Inducible and Reversible Mouse Model for Ubiquitous Recombinase Activity

Abstract: Developing lymphocytes express the recombination activating genes (RAGs) 1 and 2 products that form a site specific recombinase complex (RAG), introducing double strand DNA breaks (DSBs) at recombination signal sequences (RSSs) flanking the V, D, and J gene segments in the antigen receptor loci. The subsequent steps in the reaction consist in the ligation of DSBs by ubiquitous enzymes of the non-homologous end joining DNA repair pathway. This mutagenesis process is responsible for the generation of the very la… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2020
2020
2020
2020

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 43 publications
(47 reference statements)
0
1
0
Order By: Relevance
“…Despite their apparent advantage for vertebrate adaptive immunity, self-DNA mutating enzymes are potentially harmful and hence their activities must be strictly controlled in order to avoid collateral damage to the host genome [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. Because of their inherent proliferative capacity, lymphocytes are particularly susceptible to such off-target effects.…”
Section: Introductionmentioning
confidence: 99%
“…Despite their apparent advantage for vertebrate adaptive immunity, self-DNA mutating enzymes are potentially harmful and hence their activities must be strictly controlled in order to avoid collateral damage to the host genome [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. Because of their inherent proliferative capacity, lymphocytes are particularly susceptible to such off-target effects.…”
Section: Introductionmentioning
confidence: 99%