Iridium‐dispersed carbon paste enzyme electrodes

Wang, Joseph; Rivas, Gustavo A.; Chicharro, Manuel

doi:10.1002/elan.1140080506

Cited by 48 publications

(34 citation statements)

References 10 publications

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“…To obtain an effective detection of hydrogen peroxide without the interference of easily oxidizable compounds it would be necessary to work at potentials not higher than ¹0.1 V. That is, it would be necessary to measure the hydrogen peroxide reduction instead of the oxidation, as in the case of other metallized carbon electrodes [11][12][13]. However, at potentials more negative than ¹0.1 V, for high glucose concentrations, there is an 'anodic' signal (not shown) similar to the results obtained with CPE containing Rh and lactate oxidase [18].…”

Section: Influence Of Gold On the Electrochemical Behavior Ofmentioning

confidence: 99%

“…Metallized carbon paste electrodes have been shown to couple the high electrocatalytic efficiency of metal microparticles dispersed in carbon [14] to the known advantages of carbon paste surfaces [15]. Highly selective glucose biosensors have been obtained in the case of Rh-, Ru-, and Ir-GOx-CPEs [11][12][13].…”

Section: Introductionmentioning

confidence: 98%

“…They consist of carbon transducers containing platinum [9], palladium [10], rhodium [11], ruthenium [12] or iridium [13] electrodeposited on carbon electrodes or incorporated into the carbon paste. Such electrodes rely on the use of metallized graphite powder with a strong adherence of metal centers to the graphite particles, as well as on the electrodeposition of metals and GOx at carbon fiber or glassy carbon.…”

Section: Introductionmentioning

confidence: 99%

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Amperometric Glucose Biosensor Based on Gold-Dispersed Carbon Paste

Celej

Rivas

1998

Electroanalysis

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Metallized carbon paste electrodes containing glucose oxidase have demonstrated to be very useful for the rapid, selective, and sensitive glucose quantification. This article focuses on the development of gold-dispersed carbon paste electrodes containing glucose oxidase for glucose quantification. The catalytic action of the gold microparticles towards the electrooxidation and electroreduction of the enzymatically generated hydrogen peroxide offers a sensitive and selective monitoring of glucose. The influence of the working potential and paste composition on the analytical response and on the kinetic parameters of the bioelectrode is evaluated. The usefulness of the biosensor in flow systems is also demonstrated.

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Section: Influence Of Gold On the Electrochemical Behavior Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Amperometric Glucose Biosensor Based on Gold-Dispersed Carbon Paste

Celej

Rivas

1998

Electroanalysis

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“…The latter remains the most common form of biosensor despite requiring a large overpotential at which reducing agents present in the sample can contribute to the faradaic current. Although some of the problems associated with anodic H 2 O 2 detection have been minimized by using electrocatalytic H 2 O 2 reduction at low potentials (Gao et al, 1992;Vreeke et al, 1992;Wang et al, 1994Wang et al, , 1996, the question of sensitivity to pO 2 appears in an additional form */the possibility of direct faradaic reduction of O 2 .…”

Section: Introductionmentioning

confidence: 99%

Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose

Dixon

Lowry

O’Neill

2002

Journal of Neuroscience Methods

108

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The oxygen dependence of a first generation amperometric biosensor was investigated in vitro and in vivo by monitoring its glucose response as a function of solution pO 2 . The biosensor was a glucose oxidase (GOx) modified poly(o -phenylenediamine) coated Pt cylinder electrode (Pt/PPD/GOx) that has been designed for neurochemical analysis in vivo. Two types of oxygen probes were used: a self-calibrating commercial macroelectrode in vitro; and a carbon paste microelectrode in vivo. Calibrations in vitro showed that oxygen interference in the operation of Pt/PPD/GOx electrodes was minimal for concentrations of glucose ( Â/0.5 mM) and oxygen ( Â/50 mM) found in brain ECF. This observation was confirmed by simultaneous monitoring in vivo of brain glucose and oxygen in the awake rat. However, at levels of glucose normally found in peripheral tissues ( Â/5 mM), the oxygen dependence was severe. We conclude that the oxygen sensitivity of Pt/PPD/GOx biosensors does not preclude their reliable use in media containing low glucose levels, such as brain ECF. #

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“…However, this overpotential can be lowered by using metals such as platinum [5,6], rhodium [7,8], palladium [9±11], ruthenium [12], gold [13] and iridium [14,15]. In some cases, enzyme electrodes were fabricated by codeposition of the metal and GOx onto carbon electrodes.…”

Section: Introductionmentioning

confidence: 99%

Glucose Biosensor Prepared by the Deposition of Iridium and Glucose Oxidase on Glassy Carbon Transducer

Rodrı́guez

Rivas

1999

Electroanalysis

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A ®rst generation glucose biosensor based on the electrodeposition of iridium and glucose oxidase (GOx) on pretreated glassy carbon is described. The strong electrocatalytic action of iridium towards hydrogen peroxide allows fast glucose quanti®cation at very low potentials where the interference of easily oxidizable compounds such as ascorbic and uric acids is minima. The in¯uence of electrode pretreatment, plating solution composition and deposition time on the analytical performance and kinetic behavior of the bioelectrodes is studied. In all the cases the bioelectrodes response was measured using hydrogen peroxide and glucose in order to evaluate how the experimental conditions affect both iridium and GOx deposition. A long-term stability study showed that the sensitivity remains around 60 % of its original value up to forty-®ve days. The results demonstrated that the diminution in the activity of the bioelectrode is due to the decrease in the biocatalytic activity and not to iridium leaching. The bioelectrodes were used for measuring the glucose content in a commercial physiological solution and the results showed a relative error of 6.0 % with the label claim.

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Iridium‐dispersed carbon paste enzyme electrodes

Cited by 48 publications

References 10 publications

Amperometric Glucose Biosensor Based on Gold-Dispersed Carbon Paste

Amperometric Glucose Biosensor Based on Gold-Dispersed Carbon Paste

Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose

Glucose Biosensor Prepared by the Deposition of Iridium and Glucose Oxidase on Glassy Carbon Transducer

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