Iridovirus‐like infection associated with high mortalities of striped beakperch, <i>Oplegnathus fasciatus</i> (Temminck et Schlegel), in southern coastal areas of the Korean peninsula

Jung, Sung‐Ju; Oh, Myung‐Joo

doi:10.1046/j.1365-2761.2000.00212.x

Cited by 183 publications

(115 citation statements)

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“…Hyatt et al (2000) regarded of uncertain taxonomic position the iridovirus-like particles recovered from South East Asian freshwater and marine fish that produce hypertrophic cells in the lamina propria and epidermis, and within organs such as kidney and spleen (Armstrong & Ferguson 1989, He et al 2000, Jung & Oh 2000. The systemic iridoviral infection reported in our study found in fish, originally imported from South East Asia, similarly induces hypertrophy of the infected host cells.…”

Section: Discussionsupporting

confidence: 56%

Iridovirus infections in farm-reared tropical ornamental fish

Paperna¹,

Vilenkin²,

Matos³

2001

Dis. Aquat. Org.

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A systemic viral infection in both gourami Trichogaster spp. and swordtail Xiphophorus hellerii and an outbreak of lymphocystis in scalare Pterophyllum scalarae and gourami are reported to have occurred in fish reared in ornamental fish farms in Israel. The systemic infection developed in endothelial cells that became hypertrophic and their contents were modified. The presence of such cells in light-microscopically examined stained smears and sections provides an initial indication for this systemic viral infection. Infection in gourami caused hemorrhagic dropsy. Transmission electron microscopic (TEM) images of iridovirus-like particles recovered from gouramies showed them to be 138 to 201 nm from vertex to vertex (v-v); those from swordtails were 170 to 188 nm v-v. TEM images of lymphocystis virions from scalare were 312 to 342 nm v-v and from gourami 292 to 341 nm v-v. Lymphocystis cells from the gourami were joined by a solid hyaline plate, which was lacking in the infection in scalare where the intercellular spaces between the lymphocystis cells consisted of loose connective tissue.KEY WORDS: Trichogaster spp. · Xiphophorus spp. · Pterophyllum scalarae · Iridovirus-like particles · Systemic infection · Endothelium · Hypertrophy · Lymphocystis Resale or republication not permitted without written consent of the publisherDis Aquat Org 48: [17][18][19][20][21][22][23][24][25] 2001 findings together with a demonstration of viral particles by electron microscopy prove to be useful in the differential diagnosis of causes of mortality in the farmed fish. MATERIALS AND METHODSSmears and touch preparations were air-dried, fixed with absolute methanol and stained with Giemsa (in pH 7.4 buffer, for 1 h). For light microscopic histology, tissues were fixed in neutral-buffered formalin (exceptionally with aqueous Bouin's), and after dehydration in graded ethanols were embedded in glycol-methacrylate medium (Agar Scientific, Ltd, Stansted, UK). Sections, 2 to 3 µm, were cut with a glass knife on a Sorval JB4 microtome, and were stained with either Meyer's hemalum-eosin or, after a 20 min incubation in Bouin's solution and a water and 70% ethanol rinse, with 10% Giemsa in phosphate buffer, pH 7.4.For transmission electron microscopy (TEM), fragments from tissue with conspicuous cytopathological changes were fixed in 2.5% glutaraldehyde in cacodylate buffer (0.1 M, pH 7.4) for 24 h at 4°C, rinsed repeatedly in the same buffer, post-fixed in 1.0% osmium tetroxide in the same buffer for 1 h and, after rinsing in the buffer, dehydrated in graded ethyl alcohols and embedded in Agar 100 medium (Agar Scientific, Ltd). Thin sections, cut on a Reichert Ultracut Microtome with a diamond knife, were stained on grid with uranyl acetate and lead citrate, and examined with a Jeol 100CX TEM. Semi-thin sections cut with a glass knife were stained with Toluidine blue. RESULTS Viral hemorrhagic dropsy (VHD) in gouramiAffected fish, observed on several occasions in stocks of farmed gourami of the species Trichogaster trichopter...

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Section: Discussionsupporting

confidence: 56%

Iridovirus infections in farm-reared tropical ornamental fish

Paperna¹,

Vilenkin²,

Matos³

2001

Dis. Aquat. Org.

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“…From the morphology and localization of these cells, they were considered leukocytes. Similar viruses have been described for a number of fishes including ALIV from African lampeye, GIV from grouper Epinephelus malabaricus, SBIV from sea bass , an RSIV-like iridovirus from striped beakperch Oplegnathus fasciatus (Jung & Oh 2000, Kim et al 2002, and ISKNV from the mandarinfish Siniperca chuatsi (He et al 2001). These viruses were proposed to form a separate taxonomic unit, and were collectively termed 'cell hypertrophy iridoviruses' (He et al 2001).…”

Section: Discussionmentioning

confidence: 77%

Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)

Chao¹,

Chen²,

Lai³

et al. 2004

Dis. Aquat. Org.

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“…Among the infectious diseases, viral diseases are the most serious since they cause severe losses to fish aquaculture production. Many viral diseases of fish have been reported worldwide [66,92,108] of which infection caused by betanodavirus is a major concern. The virus has emerged as a major constraint to fish culture and is responsible for catastrophic losses reported worldwide.…”

Section: Introductionmentioning

confidence: 99%