Iron and outer membrane proteins in the susceptibility of Neisseria meningitidis to human serum

Criado, M.T.; Rio, Marie‐Christine; Ferreirós, C.M.; Pintor, M.; Sainz, V.; Carballo, Javier

doi:10.1111/j.1574-6968.1990.tb13968.x

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…As iron restriction seems to be related to the greater expression of proteins binding to transferrin 1 and 2 ( Pintor et al . 1993 ; Criado et al . 1990 ), we deduce that alterations in the cell surface hydrophobicity of N. meningitidis arising from high concentrations of melatonin and an excess of iron are not determined by these proteins.…”

Section: Discussionmentioning

confidence: 99%

The influence of different concentrations of melatonin on the cell surface hydrophobic characteristics of Neisseria meningitidis

Uberos

Molina

Liébana

et al. 2000

Lett Appl Microbiol

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The cell surface hydrophobicity of micro‐organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an expression of the virulence of Neisseria meningitidis, how its hydrophobic characteristics were affected by exposure to increasing concentrations of melatonin. An increase in the cell surface hydrophobicity of N. meningitidis was found at concentrations of 1 mmol l−1, while lower concentrations of melatonin did not significantly affect this particular cell surface characteristic of the micro‐organism. It may be concluded that melatonin clearly influences the cell surface hydrophobicity of N. meningitidis, a circumstance that should be taken into account in future studies to determine whether this hormone plays a role in the variable pathogenicity of the bacteria in different hosts.

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Section: Discussionmentioning

confidence: 99%

The influence of different concentrations of melatonin on the cell surface hydrophobic characteristics of Neisseria meningitidis

Uberos

Molina

Liébana

et al. 2000

Lett Appl Microbiol

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“…Therefore, the assay is not suited for largescale screening of complement resistance. We investigated whether the less laborious culture-and-spot assay (1,7,9) could replace the serum bactericidal assay to test large numbers of M. catarrhalis strains. In contrast to studies reviewed in reference 24, we did not encounter problems with the anticomplementary activity of 5% sheep blood agar.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the results were very easy to interpret. To date, the culture-and-spot test has been used to establish complement resistance in Escherichia coli (1) and Neisseria meningitidis strains (7). In addition, only limited data on the comparability of the culture-and-spot test and the serum bactericidal assay are available (1,9).…”

Section: Discussionmentioning

confidence: 99%

“…(ii) Culture-and-spot test. The culture-and-spot test is a modification of an assay previously described by Criado et al (7). M. catarrhalis was grown overnight in a shaking incubator at 37ЊC in Mueller-Hinton broth (Difco) containing 0.5% BSA to prevent clumping of the bacteria during growth.…”

Section: Methodsmentioning

confidence: 99%

“…The purpose of the present study was to investigate whether the serum bactericidal assay could be replaced with a less laborious ''culture-and-spot'' test (1,7,9). The latter is based on the survival of bacteria on a blood agar plate after the application of a drop of 50% serum.…”

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confidence: 99%

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Assessment of complement-mediated killing of Moraxella (Branhamella) catarrhalis isolates by a simple method

Verduin

Hol

Dijke

et al. 1995

Clin Diagn Lab Immunol

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Recently, we showed that complement resistance is an important virulence factor of Moraxella (Branhamella) catarrhalis. Our study used a serum bactericidal assay to determine complement resistance in M. catarrhalis. Although the serum bactericidal assay is considered the ''gold standard'' for determining complement resistance, it is laborious and time-consuming and therefore not well suited for large-scale studies. Using a large number (n ‫؍‬ 324) of M. catarrhalis isolates obtained from the sputa of patients with lower respiratory tract infections (n ‫؍‬ 200) and young carriers (n ‫؍‬ 124), we assessed the value of a simple ''culture-and-spot'' test as an alternative to the serum bactericidal assay. For both groups of isolates, the degree of concordance between the two tests used was very significant (P < 0.0001). The agreement between the two assays was estimated to be ''excellent beyond chance'' (as determined by Cohen's kappa test). The culture-and-spot assay is a valuable alternative to the serum bactericidal assay, not only for screening purposes as shown here but also for studying the mechanism of complement resistance in M. catarrhalis at the molecular level.

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Surface antigen analysis of group B Neisseria meningitidis outer membrane by monoclonal antibodies: Identification of bactericidal antibodies to class 5 protein

Danelli

Batoreu

Lacerda

et al. 1995

Current Microbiology

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Twenty-four monoclonal antibodies (mAbs) against group B Neisseria meningitidis surface antigens were analyzed by immunoenzymatic assays and by a bactericidal test. Two mAbs were specific to polysaccharide B and one to lipopolysaccharide. The others were specific to polysaccharide B and one to lipopolysaccharide. The others were directed against outer membrane proteins ranging in molecular mass from 25 to 200 kDa. The outer membrane protein epitopes recognized by the mAbs were not conformational and were located on the outer surface of the microorganism. Linear epitopes on the class 5 protein, exposed on the surface of the membrane, were able to induce bactericidal antibodies to the homologous strain. The susceptibility of Neisseria meningitidis to these antibodies was unchanged when this organism was cultivated under conditions of iron depletion. These results demonstrate that peptides derived from class 5 proteins are potentially important in synthetic peptide or in recombinant protein vaccines containing linear bactericidal epitopes.

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Iron and outer membrane proteins in the susceptibility of Neisseria meningitidis to human serum

Cited by 7 publications

References 30 publications

The influence of different concentrations of melatonin on the cell surface hydrophobic characteristics of Neisseria meningitidis

The influence of different concentrations of melatonin on the cell surface hydrophobic characteristics of Neisseria meningitidis

Assessment of complement-mediated killing of Moraxella (Branhamella) catarrhalis isolates by a simple method

Surface antigen analysis of group B Neisseria meningitidis outer membrane by monoclonal antibodies: Identification of bactericidal antibodies to class 5 protein

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